Recombinant Human IL-1 beta/IL-1F2
R&D Systems Recombinant Human IL-1 beta/IL-1F2 IL-1 is a name that designates two pleiotropic cytokines, IL-1α (IL-1F1) and IL-1β (IL-1F2), which are the products of distinct genes. IL-1α and IL-1β are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. S…
The supplier does not provide quotations for this product through SelectScience. You can search for similar products in our Product Directory.
R&D Systems Recombinant Human IL-1 beta/IL-1F2
IL-1 is a name that designates two pleiotropic cytokines, IL-1α (IL-1F1) and IL-1β (IL-1F2), which are the products of distinct genes. IL-1α and IL-1β are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins.
Specifications and Use of R&D IL-1 beta / IL-1F2
Source
• A DNA sequence encoding the mature human IL-1β protein (Ala 117 - Thr 269; Accession # NP_000567) was expressed in E. coli.
Molecular Mass
• The 153 amino acid residue recombinant protein has a predicted molecular mass of approximately 17 kDa. The 154 amino acid residue methionyl form may also be present in the preparation.
Purity of R&D IL-1 beta / IL-1F2
• > 97%, as determined by SDS-PAGE and visualized by silver stain.
Endotoxin Level
• < 1.0 EU per 1 μg of the cytokine as determined by the LAL method.
Activity
• Measured in a cell proliferation assay using the murine helper T cell line, D10.G4.1 (Symons, J.A. et al., Lymphokines and Interferons, a Practical Approach, 1987, M.J. Clemens, A.G. Morris, and A.J.H.
Gearing, eds., IRL Press, p. 272).
• The ED50 for this effect is typically < 12 pg/mL.
Formulation
• Lyophilized from a 0.2 μm filtered solution in PBS containing 50 μg of bovine serum albumin per 1 μg of cytokine.
Reconstitution of R&D IL-1 beta / IL-1F2
• It is recommended that sterile phosphate-buffered saline containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 10 μg/mL of the cytokine.
Storage of R&D IL-1 beta / IL-1F2
• Lyophilized samples are stable for up to twelve months from date of receipt at -20° C to -70° C.
• Upon reconstitution, this cytokine can be stored under sterile conditions at 2° - 8° C for one month or at -20° C to -70° C in a manual defrost freezer for three months without detectable loss of activity.
• Avoid repeated freeze-thaw cycles.
