RNase R
Digests linear RNA and Y-structure RNA, leaving circRNA, lariat intron RNA, and dsRNA intact. Ideal for isolating circRNA from total RNA preparations. 1 µg of RNase R digests 5 µg of linear RNA in 30 minutes.
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Ribonuclease R (RNase R) from E. coli, is a 3’→5’ exoribonuclease that can digest linear RNA and Y-structure RNA, but does not digest circular RNA (circRNA), lariat intron RNA, blunt-ended double-stranded RNA (dsRNA), dsRNA with 3’-overhangs <7 nt in length or single-stranded DNA. Highly structured linear cellular RNAs such as prokaryotic 23S and 16S ribosomal RNAs (rRNAs), small nuclear RNAs (snRNAs), transfer RNAs (tRNAs) and histone mRNAs are also not efficiently degraded by RNase R. This functionality makes RNase R ideal for isolating circular RNA structures from total cellular RNA preparations for use in intron and splicing studies and natural cellular circRNA identification. Additionally, RNase R is used as a crucial component in in vitro circRNA preparation cleanup and enrichment with both RNA Ligase-based or self-splicing permutated intron–exon sequence (PIE method)-based methodologies. Product is for research use only (RUO).
