RNase R
Enzyme supplied with 10X Reaction Buffer.
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Great results and easy to use.
Remove linear RNA
To amplify circular RNAs, it needs to remove the linear RNAs. To do this, I used RNase R from Applied Biological Materials Inc. It is easy to use and provides high quality and reproducible results. I recommend this to remove the linear RNAs.
Review Date: 5 Nov 2018 | Applied Biological Materials Inc.
RNase R is an E. coli exoribonuclease which exhibits 3'-to-5' exonuclease activity, efficiently digesting nearly all linear RNA species. This enzyme does not digest circular, lariat, or double stranded RNA with short 3’ overhangs (less than seven nucleotides). As such, this enzyme is ideally suited to the study of lariat RNA produced by traditional splicing, as well as circRNAs which arise through back-splicing. By removing linear RNAs from cellular or RNA extracts, RNase R greatly facilitates the identification of circular species through RNA-sequencing. This enables researchers to probe the landscape of splicing events with greater depth.
Applications:
- Enriching circRNAs in biological samples
- Identification of intronic lariat sequences
- Identification of exonic circRNAs
- Studying alternative splicing
- Production of artificial circular RNAs
