SaltRx
SaltRx was developed by Hampton Research as a primary and secondary, salt and pH based crystallization screen for biological macromolecules. Salt is the only primary crystallization reagent (precipitant) utilized. Based on a design of 96 conditions, the screen evaluates a broad portfolio of crystallization salts of varying concentration and pH.SaltRx can be used as a primary crystallization screen when salt, ionic strength an…
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Proteins Crystallization And Solubility
The Salt Rx formulated by Hampton Research is good for screening the initial crystallization. It is also useful for finding a good solubility solution for the protein of interest. This screen together contains 96 solutions with different concentration of salts and also with varying pHs. Besides crystallization these screen provide an excellent opportunity to find a good buffer for the protein of interest. I recommend these screen to be used for both crystallization and checking solubility of the protein of interest.
Review Date: 8 Jan 2014 | Hampton Research
SaltRx was developed by Hampton Research as a primary and secondary, salt and pH based crystallization screen for biological macromolecules.
Salt is the only primary crystallization reagent (precipitant) utilized. Based on a design of 96 conditions, the screen evaluates a broad portfolio of crystallization salts of varying concentration and pH.
SaltRx can be used as a primary crystallization screen when salt, ionic strength and pH are desired or suspected as appropriate crystallization variables. It is also useful as a secondary screen when salt only reagents/conditions from screens such as Index™, Crystal Screen™, and Grid Screen™ produce crystals and when further screening for additional salt conditions or optimization is desired.
Since this screen does not contain volatile organics, it is compatible with microbatch, vapor diffusion, liquid and gel diffusion crystallization methods.
SaltRx Features:
- Salt versus pH matrix crystallization screen
- Samples pH 4 to 9
- 22 unique salts versus salt concentration and pH
- Compatible with microbatch, vapor diffusion, liquid & gel diffusion methods
- Tube or Deep Well block format
- Preformulated, ready to screen
- Developed at Hampton Research
- All salts and buffers in screen readily available as Optimize™ reagents for reproducing and optimizing crystals
Applications:
- Primary or secondary, salt and pH matrix crystallization screen for biological macromolecules
