SapphireAmp® Fast PCR Master Mix
SapphireAmp Fast PCR Master Mix is a hot start PCR master mix that contains a hot start PCR enzyme, optimized buffer, dNTP mixture, gel loading dye (blue), and a density reagent in a convenient 2X premix. SapphireAmp PCR Master Mix is especially designed for fast PCR reactions that can be completed in half the time required when using conventional Taq DNA polymerase. For human genomic DNA, amplification of 2 kb targets can be…
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Has transformed my colony PCR workflow.
Fast colony PCR
I have used this product weekly for around 2.5 years. It is very good for amplifying dirty samples e.g. bacteria in a colony PCR. The PCR program I use takes only 28 minutes, and I get bright bands every time, even with difficult templates.
Review Date: 30 Oct 2020 | Takara Bio
Best for colony PCR.
Colony PCR
SapphireAmp is the best for regular colony PCR to identify positive colonies after transformation. It is (1) Fast (10 sec extention/kb), (2) Robust (up to 6 kb), and (3) Cost effective. In my lifetime, the success rate is >99% with >1000 samples!
Review Date: 29 Apr 2020 | Takara Bio
SapphireAmp Fast PCR Master Mix is a hot start PCR master mix that contains a hot start PCR enzyme, optimized buffer, dNTP mixture, gel loading dye (blue), and a density reagent in a convenient 2X premix.
SapphireAmp PCR Master Mix is especially designed for fast PCR reactions that can be completed in half the time required when using conventional Taq DNA polymerase. For human genomic DNA, amplification of 2 kb targets can be completed in 1 hour, and amplifications of up to 6 kb are possible.
This hot start PCR master mix product is especially well-suited for E. coli-based colony PCR to screen inserts up to ~5 kb in length. Colony PCR with SapphireAmp Master Mix can be completed in about 1 hour.
SapphireAmp reactions can be assembled by simply adding primers and DNA template to the hot start PCR master mix. Reactions can be analyzed directly without purification; PCR products can be loaded directly onto a gel for electrophoresis or digested with restriction enzymes in the SapphireAmp reaction buffer.
Features:
- Hot start PCR master mix is optimized for fast PCR
- Colony PCR can be completed in half the time required for conventional Taq DNA polymerase
- Convenient- eliminates purification steps with no reduction in yield
- Restriction enzyme digestion of the PCR products can be performed directly in the PCR buffer
- Reactions can be directly loaded onto a gel without purification or the addition of other reagents
Applications:
- Hot start PCR
- Colony PCR
- Fast PCR
