scPicking Platform – Single Cell Visualisation and Isolation

The scPicking Platform – the easiest way to isolate the single cells that matter to you

The single-cell Picking Platform consists of two core instruments – the isoPick and isoHub, with optional imaging and fluorescence capabilities available.

isoPick - gently isolate and pick your single cells of interest
The isoPick automates all fluid handling for the dispensing, isolation and transfer of single cells. Using our GRID technology, this instrument gently dispenses cells into nanolitre chambers for easy visualisation without optical edge effects, then flexibly transfers selected single cells into a range of different formats. Compatible with a range of downstream applications, the isoPick is a flexible instrument that enables the isolation of the single cells that matter to you. Optional sample cooling and ultra-gentle handling of sensitive cells ensures maximum cell viability during the isolation and transfer process.

isoHub - easily visualise and verify single cells
Chambers can be easily viewed without any of the optical edge effects typically associated with conventional culture plasticware e.g. 96-well plates. Using our GRID technology, you have absolute confidence in identifying chambers that contain a single cell immediately after plating. The isoHub automates navigation through chambers and allows visualisation of cells using either using brightfield or fluorescent mode (if available). Data is wirelessly transferred between the isoPick and isoHub, guaranteeing a seamless and efficient workflow without the need to manually note single-cell chamber coordinates prior to transfer.

Image Capture System - document single cells with whole-chamber images
The optional Image Capture System lets you document single cells with just a click using whole-chamber imaging. Simply select GRID chambers that contain a single cell, and the imaging system records and documents your selection with a whole-chamber image, enabling you to link future results from your downstream single-cell analyses back to your single-cell images.

isoHub Fluorescence - 4 channels and 16 LED wavelengths to detect any fluorophore
The scPicking Platform optionally features a CoolLED pE-4000 illumination system for fluorescence microscopy. Easily detect commonly used fluorescent molecules (GFP, RFP, DAPI, and more) thanks to the system’s 4 channels, 16 LED wavelengths, and a user-friendly control pad. With simple one button on/off operation, it couldn’t be easier.

Feature highlights:

• Reproducible and consistent results with powerful automation
• Highest assurance of single cell isolation and transfer – up to 100%
• Low pressure of <0.5 psi ensures cell handling is incredibly gentle and maintains exceptional cell viability
• Single cells are easily identified with a simple click of a button immediately after plating, in a single field of view without any optical edge effects
• Easily navigate the entire single-cell isolation workflow with an intuitive touchscreen
• Flexible cell transfer into different formats - 96-well TC plates, 96-well PCR plates, 8-tube PCR strips, or LC-MS vials
• Optional sample cooling
• Image cells as desired via imaging (optional)
• Select cells using fluorescence (optional)

Suitable for downstream applications such as:

• Single-cell cloning
• Single-cell genomics
• Single-cell lipidomics
• Microalgae isolation
• And many more – get in touch to discuss your requirements