Single Cellome™ Unit SU10
SU10 is a nanopipette-based tool for efficient and controlled delivery of material (such as CRISPR Cas9 complex, RNA, proteins) into the nucleus or cytoplasm of single cells in a highly automated manner. The CRISPR Cas9 complex can be delivered directly into the nucleus of a specific target cell in a minimally invasive manner through the utilized glass pipette in nanometer range (approx.100 nm).
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SU10 is a nanopipette-based tool for efficient and controlled delivery of material (such as CRISPR Cas9 complex, RNA, proteins) into the nucleus or cytoplasm of single cells in a highly automated manner. The CRISPR Cas9 complex can be delivered directly into the nucleus of a specific target cell in a minimally invasive manner through the utilized glass pipette in nanometer range (approx.100 nm). Especially the delivery of material into cells that are hard-to-transfect can be a benefit by using SU10.
Key features
- Low Invasiveness: Glass pipette with tip size of under 100 nm
- Automated Penetration: Automated cell surface detection and penetration (Z direction movement)
- Automated Injection: Automated, controller volume injection using electro-osmotic flow
- High Success Rate: Approx. 95% success rate of injection*3
- Single-Cell Targeting: Enabled injection of selected cells under microscope observation
- Rapid Injection: Capable of injecting one cell every 10 seconds*3
*1 Function to aspirate intracellular substances is under development.
*2 Microscope sold separately.
*3 Experiment by Yokogawa.
Applications
- Direct injection of substances such as vector and genome editing tools (CRISPR/Cas9) into the nucleus
- Efficacy/toxicity evaluation of drug candidate molecules
- Other physical injection of reagents and proteins
- Inject into cells that cannot be introduced by conventional methods
- Analysis of cell-cell communication starting from the injected cell
