SMARTer Stranded Total RNA Sample Prep Kit

SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian—Strand-Specific RNA-Seq Libraries for Illumina Platforms from High-Input Total RNA.

The SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian provides a rapid solution for preparing indexed Illumina® sequencing libraries from 100 ng–1 µg of mammalian total RNA. This kit combines key RiboGone and SMART technologies, seamlessly blending efficient rRNA removal and strand-specific library generation in around 5 hours. Because rRNA comprises ~90% of total RNA, effective depletion of these abundant transcripts prior to library generation can lower sequencing costs and improve mapping statistics.

The core SMART technology completes a streamlined process that maintains strand information, and eliminates downstream library prep by incorporating indexes and adapters during the reverse transcription and PCR amplification steps.


Features:

• Strand information—Identify each transcript’s strand of origin with >99% accuracy.
• Fast, streamlined protocol—Go from start to finish in ~5 hours.
• Large input size—Use 100 ng–1 µg total RNA from human, mouse, or rat.
• Versatility—Get reliable data across replicates and RNA quality. See high-quality, reproducible data from total RNA with a RIN (RNA Integrity Number) from 3–10.
• Integrated with Illumina sequencing—Generate Illumina-specific libraries with up to 96 indexes, and retain strand information.

Applications:

• Illumina-specific NGS library generation that retains strand information
• RNA-Seq on Illumina platforms for mammalian samples
• Coding and non-coding transcript information