StarBright™ Blue 520 Fluorescent Secondary Antibodies
The StarBright Blue 520 Fluorophore is an ultra-sensitive polymer-based fluorophore with an excitation maximum at 488 nm and emission maximum at 520 nm. The StarBright Fluorophore is made up of multiple light-absorbing and -emitting monomers which provide an exceptionally bright signal compared to most traditional fluorophores.
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It is a good advancement and needs to be used more.
Stem cell regenerative medicine
The product is good and can be used for multiple uses defining antigenic profiles.
Review Date: 14 Mar 2019 | Bio-Rad
Great reagent.
Biochemistry
Increase sensitivity, reduce background signal and compatible with other fluorescent Abs.
Review Date: 18 Feb 2019 | Bio-Rad
It is a good asset for the lab.
Antibody purification
Effective product for protein separation.This was used for purification of desired antibodies raised against a specific antigen and gave me good yield.
Review Date: 18 Feb 2019 | Bio-Rad
Great product.
Immunoblotting
It enables high-quality immunoblotting results.
Review Date: 18 Feb 2019 | Bio-Rad
Helps me in my area of work.
Western blotting
I recommend, easy to use with protocol. Less time consuming than others.
Review Date: 18 Feb 2019 | Bio-Rad
StarBright Blue 520 Conjugated Goat Anti-Mouse and Goat Anti-Rabbit Fluorescent Secondary Antibodies are ideal for fluorescent western blotting — either for the detection of a single-target protein or for multiplex detection of several proteins on one blot, without stripping and reprobing. StarBright Blue 520 Fluorescent Secondary Antibodies can be used with far-red emission StarBright Blue 700 Antibodies as well as traditional fluorescent detection reagents like Alexa Fluor and DyLight Conjugated Antibodies. In addition, StarBright Antibodies can be used with Bio-Rad's stain-free technology and/or with hFAB™ Rhodamine Anti-Housekeeping Primary Antibodies for protein normalization. These antibodies are optimized for use with the ChemiDoc™ MP Imaging System, permitting detection of up to three proteins in a single blot.
Features and Benefits
- Easy multiplexing — can be used with traditional fluorescent antibodies or with Bio-Rad's StarBright Blue 700 Fluorescent Secondary Antibodies to detect multiple proteins on the same blot
- High sensitivity — StarBright Blue 520 Fluorescent Secondary Antibodies (Exmax/Emmax = 488 nm/520 nm) have 2–4 fold higher sensitivity compared to traditional green emitters like Alexa Fluor 488 or DyLight 488 Antibodies
- Short exposure time — the exceptional brightness of the StarBright Fluorophore leads to shorter exposure times compared to traditional fluorescent dyes: seconds vs. minutes
- Low nonspecific binding — the StarBright Fluorophore is conjugated to highly cross-adsorbed antibodies
Applications and Uses
StarBright Blue 520 Fluorescent Secondary Antibodies can be used for multiplex fluorescent western blotting with Bio-Rad's ultra-sensitive near IR StarBright Blue 700 Antibodies and the hFAB Rhodamine Anti-Housekeeping Antibodies as described in the table below.
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Products Model Information
StarBright™ Blue 520 Goat Anti-Mouse IgG, 400 µl
Bio-RadPkg of 1, lyophilized goat anti-mouse IgG conjugated with StarBright Blue 520 Fluorophore; reconstitute to 400 µl
StarBright™ Blue 520 Goat Anti-Mouse IgG, 80 µl
Bio-RadPkg of 1, lyophilized goat anti-mouse IgG conjugated with StarBright Blue 520 Fluorophore; reconstitute to 80 µl
StarBright™ Blue 520 Goat Anti-Rabbit IgG, 400 µl
Bio-RadPkg of 1, lyophilized goat anti-rabbit IgG conjugated with StarBright Blue 520 Fluorophore; reconstitute to 400 µl
StarBright™ Blue 520 Goat Anti-Rabbit IgG, 80 µl
Bio-RadPkg of 1, lyophilized goat anti-rabbit IgG conjugated with StarBright Blue 520 Fluorophore; reconstitute to 80 µl