StepOnePlus™ Real-Time PCR System
The StepOnePlus™ Real-Time PCR System is a 96-well Real-Time PCR instrument perfect for both first-time and experienced users. Applied Biosystems’ latest introduction to real-time PCR, the StepOnePlus™ Real-Time PCR System, makes it simple and easy to get publication-ready high-quality real-time PCR results on day one. This remarkably simple 96 well, 4 color, real-time PCR system is designed with a user-friendly, yet powerful…
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Thermocycles for good
Diagnosis of plant pathogens
StepOnePlus thermocyclers aren't new products, but I’d like to highlight how we’ve been using two units from this brand for about 15 years, and they continue to perform as expected. Despite being used heavily—an average of three to five times a day, Monday through Friday, for 15 to 16 years—maintenance has been needed only rarely. While we've encountered some issues over the years, the machines still generate reliable data. They’re user-friendly, though the software could benefit from updates over time. Overall, they've more than paid off in terms of value and performance.
Review Date: 21 Oct 2024 | Thermo Fisher Scientific
The equipment is not very intuitive. It is outdated and has an average cost
Molecular Biology
The equipment is difficult to handle, not very intuitive. It is outdated and has an average cost. It has few reading channels and the company usually refuses to carry out updates, so it is difficult for the results obtained to be reliable.
Review Date: 23 Jun 2022 | Thermo Fisher Scientific
Great results! Great investment!
Analyze possible mutations in samples
The StepOnePlus RT PCR system is easy to learn how to use. There are multiple ways it can present the data for you. I like that I can just pop it in the machine and walk away, 2 hours later you've got your results! Fantastic. Worth the investment as it saves time and lets me get more work done!
Review Date: 20 May 2022 | Thermo Fisher Scientific
Easy to use! Great results. I just love it!
Gene expression in animal and cellular samples
Very easy to use and you obtain your results very quickly. The software is also very friendly and ease to use. We analyze gene expression on a daily basis and honestly the step one plus is one of my best friends in the lab!
Review Date: 27 Mar 2022 | Thermo Fisher Scientific
Great feeling with this instrument
Cancer immunology
I like this instrument a lot because it is very sensitive and reproducible. We are using it continuously for a long time. The services from Thermo are very nice. We are also planning to purchase a another instrument like this.
Review Date: 4 Feb 2022 | Thermo Fisher Scientific
Excellent value; very useful instrument; bread and butter of our lab
qPCR ; differential expression; KO studies
The step-one plus qPCR machine is the most used equipment shared by different teams within our research program. Although it is heavily used, we never faced any technical issues and the results were reproducible run after run.
Review Date: 25 Jun 2021 | Thermo Fisher Scientific
Consistently good results.
Molecular biology
The equipment is very good and easy to operate. After sale service is also good.
Review Date: 6 Jun 2021 | Thermo Fisher Scientific
Great instrument. good customer service.
Gene expression studies
Very reliable system and results are consistent. User friendly machine, easy to setup and analyze the data.
Review Date: 19 Jun 2019 | Thermo Fisher Scientific
Easy to use and setup
PCR applications
Technical support where technical specialist is knowledgeable
Review Date: 26 Mar 2019 | Thermo Fisher Scientific
Think about another one
molecular research
There are is no difference between ABI StepOne and StepOne plus Real-Time PCR machine, apart from the second one being being more expensive. Also you need to use ROX as a reference dye with your master mixes and you can set the tests up for just 2 targets in both machines.
Review Date: 17 Jul 2018 | Thermo Fisher Scientific
The StepOnePlus™ Real-Time PCR System is a 96-well Real-Time PCR instrument perfect for both first-time and experienced users.
Applied Biosystems’ latest introduction to real-time PCR, the StepOnePlus™ Real-Time PCR System, makes it simple and easy to get publication-ready high-quality real-time PCR results on day one. This remarkably simple 96 well, 4 color, real-time PCR system is designed with a user-friendly, yet powerful, interface for researchers of all experience levels.
Instrument software for the Applied Biosystems StepOne Real-Time PCR System runs on the Windows XP® operating system and provides instrument control, data collection, and data analysis.
The StepOnePlus™ system brings advanced real-time PCR technology to a new level of accessibility. Beginning at the StepOne software homepage, you can seamlessly navigate through all aspects of the real-time PCR method including sample and reaction set-up, thermal cycling, and fluorescent detection.
The software is even pre-populated with the right run protocol for each application when using reagents from Applied Biosystems. Just enter your plate set up and go. Depending on the experimental design, the system can even help you select and order real-time PCR reagents online by means of convenient links in the Design Wizard. The StepOnePlus System is a long life LED based optical system that records fluorescence from FAM/SYBR Green, VIC/JOE, NED/TAMRA and ROX dyes for gene expression analysis, pathogen detection, SNP genotyping and presence/absence assays. This 4-color/96 well system delivers precise, quantitative real-time PCR results that you can have total confidence in.
StepOnePlus™ Real-Time PCR System Features:
- Advanced software and instrumentation for performing a wide array of genomic assays.
- Sensitive 4-color optical LED recording system.
- Intuitive and robust software perfect for first-time and advanced users.
- Simple and flexible instrument set-up and usage.
- Rapid and simple assay set-up.
- Versatile instrument configuration options.
- Energy efficiency and space saving.
- Real-time data monitoring, dissemination and storage.
- Advanced High Resolution Melting Capability
