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Swift Accel-NGS® 2S PCR-Free DNA Library Kit (96 reactions)
PCR-free NGS prep with highest library diversity, lowest inputs
Accel-NGS® 2S PCR-Free DNA Library Kit
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Eliminate sequencing bias introduced by polymerase with PCR-free preps. Enables PCR-free preparation of high complexity NGS libraries from double-stranded DNA. The kit is designed for those who have sufficient starting material to produce libraries free of bias from polymerase and for those wishing to use a polymerase of choice for amplification. PCR primers are included for users with low inputs (10 pg to 100 ng) who wish to use a polymerase of choice.
Benefits include:
- PCR-free libraries from inputs as low as 10 ng (cfDNA)
- PCR-free libraries from inputs as low as 100 ng (physically sheared DNA)
- Excellent efficiency, data quality, and evenness of coverage
- No base composition bias from AT-/GC-rich genomes
- Optional library amplification with self-selected polymerase
- Right choice for users seeking unbiased coverage from relatively low input samples, like circulating, cell-free DNA or FFPE samples
