T4 DNA Ligase
T4 DNA Ligase is a ligation enzyme that can be used to join DNA fragments by catalyzing the formation of phosphodiester bonds between juxtaposed 5' phosphate and 3' hydroxyl termini in double-stranded DNA using ATP as a coenzyme. Both blunt and cohesive end DNA ligation, as well as single-stranded nick repair of DNA, RNA, and DNA/RNA, are possible using the T4 DNA ligase.
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Molecular Cloning
The T4 DNA Ligase from Takara Bio is very effective in my research. With Takara DNA Ligase my gene cloning was successful with higher rate. Easy to use and the technical support is really helpful after sales. The team is willing to address all my inquiries through email. The price is worth for this DNA Ligase.
Review Date: 12 Feb 2022 | Takara Bio
T4 DNA Ligase is a ligation enzyme that can be used to join DNA fragments by catalyzing the formation of phosphodiester bonds between juxtaposed 5' phosphate and 3' hydroxyl termini in double-stranded DNA using ATP as a coenzyme. Both blunt and cohesive end DNA ligation, as well as single-stranded nick repair of DNA, RNA, and DNA/RNA, are possible using the T4 DNA ligase.