TB Green Premix Ex Taq (Tli RNase H Plus)
The Takara Bio TB Green Premix Ex Taq (Tli RNase H Plus), an intercalating dye-based qPCR master mix for real-time RT-PCR (qPCR), is recommended for most real-time PCR applications including gene expression analyses, genotyping, and high-throughput studies.
The supplier does not provide quotations for this product through SelectScience. You can search for similar products in our Product Directory.
Run many reactions in a day so a great product. I love it.
Insect gene expression, RNAi studies
This is a wonderful product The advantage of this product over others is that it takes 40-50 minutes for a reaction with this mix.
Review Date: 15 Feb 2018 | Takara Bio
Robust enzyme and reproducible and fast reaction settings.
Gene expression analysis by qRT-PCR
Very good product with high efficiency and accuracy. Partly due to the RNase. No worries about residual RNA present and due to the fast amplification ability of the polymerase, cycle times are less and the reaction finishes faster.
Review Date: 28 Jul 2016 | Takara Bio
The Takara Bio TB Green Premix Ex Taq (Tli RNase H Plus), an intercalating dye-based qPCR master mix for real-time RT-PCR (qPCR), is recommended for most real-time PCR applications including gene expression analyses, genotyping, and high-throughput studies. TB Green Premix Ex Taq (Tli RNase H Plus) includes TB Green, a qPCR reagent for intercalator-based RT-PCR (qPCR). In addition to being ideal for most standard real-time PCR reactions, this kit is recommended when a higher yield of product is needed or when amplifying longer products (up to 570 bp). This TB Green qPCR premix is supplied at a 2X concentration and allows easy reaction assembly; by simply adding primers, template and sterile distilled water, intercalator-based qPCR can be performed.
TB Green Premix Ex Taq (Tli RNase H Plus) contains TaKaRa Ex Taq HS, a hot start PCR enzyme that includes an anti-Taq antibody. The TaKaRa Ex Taq HS enzyme, in combination with its qPCR optimized buffer, allows for high amplification efficiency and detection sensitivity in qPCR. The 2X TB Green qPCR premix also contains Tli RNase H, a heat-resistant RNase H that minimizes qPCR inhibition as a consequence of residual mRNA in the input template cDNA. This product enables accurate detection of targets even during high-speed reactions, making it possible to conduct fast RT-PCR (qPCR) analyses and achieve excellent reproducibility and reliability.
Features:
- Lower Cq values as a result of fast extension rates
- Use with broad range of amplicon sizes, even over 500 bp
- Convenient 2X master mix is excellent for high-speed qPCR
- High sensitivity: detects fewer than 100 copies
- Hot start PCR enzyme enables high efficiency and sensitivity in real-time PCR (qPCR)
- Compatible with all commonly used real-time PCR (qPCR) instruments
- Minimizes qPCR inhibition due to residual mRNA in input cDNA
- Includes SYBR Green I for intercalator-based qPCR
Applications:
- SYBR qPCR using SYBR Green I and ROX Reference Dye
- Gene expression analysis
- Genotyping by real-time PCR
- High-throughput real-time PCR
- Amplification of long products
- When high yield is critical (e.g., during gene expression analysis of rare cDNAs)
