Thermo Scientific™ 1-Step Human Coupled IVT Kit - DNA
Thermo Scientific 1-Step Human In Vitro Protein Expression Kits enable the translation and post-transcriptional modification of full-length proteins from mRNA or plasmid templates with yields of up to 100 µg/mL per reaction. The Human IVT Kits are unique HeLa cell lysate-based protein expression systems for in vitrotranslation or coupled transcription/translation reactions. Protein expression is performed in a single 90-min…
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In vitro translation or coupled transcription/translation reactions
It is very easy to use, robust and high efficiency system for in vitro protein expression. The resulted products could be conveniently applied in down stream protein analysis such as ELISA, Western blotting, etc.
Review Date: 27 Jul 2018 | Thermo Fisher Scientific
Thermo Scientific 1-Step Human In Vitro Protein Expression Kits enable the translation and post-transcriptional modification of full-length proteins from mRNA or plasmid templates with yields of up to 100 µg/mL per reaction.
The Human IVT Kits are unique HeLa cell lysate-based protein expression systems for in vitrotranslation or coupled transcription/translation reactions. Protein expression is performed in a single 90-minute reaction that can be extended for up to 6 hours with continued protein production up to 100 µg/mL when combined with the optimized pT7CFE1 Expression Vector. The Human IVT Kits can express functional enzymes, phosphoproteins, glycoproteins and membrane proteins for immediate use in studying protein interactions, performing rapid mutational analysis and measuring activity.