Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin and Labeling Kits
The most popular amine-reactive biotinylation reagent for antibody labeling Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin and Labeling Kits Features: Water-soluble analog of NHS-LC-Biotin Reacts with primary amines at pH 7-9 in non-amine-containing buffers such as borate, carbonate or phosphate buffers Negative charge of -SO3 group keeps biotinylation localized at the cell surface Creates an amide bond Applicatio…
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Great reliable and reproducable results!
Protein
First time using this kit and it was quick, simple and reliable. I recieved an expected yield/level of biotinylation and can highly reccomend this kit.
Review Date: 27 Mar 2023 | Thermo Fisher Scientific
Surface Protein Labelling
This product is great, reliable, economical and very easy to use, especially for the application of cell surface protein labelling. I have used it to test the membrane bound EGFR, and gp130 protein levels, and it has worked every time. The only drawback is this product has is that the company doesn't provide a small aliquoted packages, so it is a pain in the neck to measure up the small amounts every time, a bit annoying. But overall it is all good.
Review Date: 8 Jan 2014 | Thermo Fisher Scientific
The most popular amine-reactive biotinylation reagent for antibody labeling
Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin and Labeling Kits Features:
- Water-soluble analog of NHS-LC-Biotin
- Reacts with primary amines at pH 7-9 in non-amine-containing buffers such as borate, carbonate or phosphate buffers
- Negative charge of -SO3 group keeps biotinylation localized at the cell surface
- Creates an amide bond
Applications:
- Enhanced detection of DNA on nitrocellulose
- eliminating the need for radioactive detection in Southern
- Northern, and dot blotting
- Biotinylation of cell surface proteins
- in vivo imaging and therapy studies
