Thermo Scientific™ Pierce™ Lys-C Protease, MS Grade
Lys-C Protease, MS Grade is a highly purified, mass-spectrometry-grade, native endoproteinase validated for maximum activity and stability in proteomic applications.
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This Lys-C is a mass spectrometry (MS)-grade serine protease isolated from Lysobacter enzymogenes. Lys-C has high activity and specificity for lysine residues resulting in larger peptides and less sample complexity than trypsin. Unlike trypsin, Lys-C can cleave lysines followed by prolines, making it ideal for sequential protein digestion followed by trypsin to decrease missed cleavages. Additionally, Lys-C prototypic peptides typically have higher charge states, making it an enzyme of choice for use with ETD fragmentation. Lys-C is commonly in phosphopeptide enrichment workflows because it generates peptides with primary amines at both the N-and C-terminus allowing the fragments to be double-labeled with amine-reactive isobaric tags. This Lys-C enzyme is packaged lyophilized (20 µg or 100 µg quantities).
Features:
- Enhanced digestion—use in tandem with trypsin to decrease tryptic missed cleavages
- Increased sequence coverage—better protein characterization results from overlapping peptides with complementary chromatographic, ionization and fragmentation properties
- Versatile—effective enzyme activity under highly denaturing conditions (e.g., 8M urea)
- C-terminal lysine cleavage specificity—at least 90% for a complex protein sample
- Stable—provided in a lyophilized format
