Thermo Scientific™ Pierce™ Rapid Gold BCA Protein Assay Kit
The Pierce Rapid Gold BCA Protein Assay Kit is a two-component protein assay to measure total protein concentration that offers high-precision, detergent-compatibility assay. Using the same copper-chelating technology as the well-known Pierce BCA Protein Assay, the Pierce Rapid Gold BCA Protein Assay provides comparable accuracy but with a 5 minute, room temperature incubation, eliminating the need to wait or expose the sample…
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Effective and reliable tool for protein quantification!
Measure total proteins concentration
I have been using the Thermo Scientific™ Pierce™ Rapid Gold BCA Protein Assay Kit for protein quantification in my research, and I must say, it has proven to be a valuable addition to my lab work: - It provides accurate and reproducible results. The variation in measurements is minimal. - The kit is relatively easy to use and the provided instructions are clear. - For those considering using this kit, it's helpful to know that thorough mixing and proper pipetting technique are crucial to obtaining accurate results. - The Thermo Scientific™ Pierce™ Rapid Gold BCA Protein Assay Kit offers good value for money. The accuracy, reproducibility, and sensitivity it provides justify the cost, especially for researchers who regularly perform protein quantification.
Review Date: 30 Oct 2023 | Thermo Fisher Scientific
The Pierce Rapid Gold BCA Protein Assay Kit is a two-component protein assay to measure total protein concentration that offers high-precision, detergent-compatibility assay. Using the same copper-chelating technology as the well-known Pierce BCA Protein Assay, the Pierce Rapid Gold BCA Protein Assay provides comparable accuracy but with a 5 minute, room temperature incubation, eliminating the need to wait or expose the samples to elevated temperatures quicker results.
Features
- Colorimetric—estimate visually or measure with a standard spectrophotometer or plate reader; produces intense orange color at 480 nm
- Excellent uniformity—exhibits similar protein-to-protein variation as traditional BCA Protein Assay, and less protein-to-protein variation than dye-binding protein assay methods
- Compatible—unaffected by typical concentrations of most ionic and nonionic detergents
- Fast—5 minute assay a, room temperature
- High linearity—linear working range for BSA equals 20 to 2000 µg/mL
The Pierce Rapid Gold BCA assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the Pierce Rapid Gold BCA assay is less affected by protein compositional differences, providing low protein-to-protein variation.
