Thermo Scientific™ Pierce™ Silver Stain Kit

The Pierce Silver Stain Kit is a metallic silver protein stain that yields a remarkably clear and uniform gel background while delivering consistent, high-sensitivity staining results at each and every use. In standard mini gels, proteins are detectable at greater than 0.25ng per band or spot. The protocol has been optimized for flexibility by allowing short or overnight gel fixation and staining steps without affecting staining performance (sensitivity or clarity). A short (one-minute) sensitization step, performed after gel fixation, yields results that are free of the characteristically dark or blotchy backgrounds often seen with homemade or other commercially available silver stains. This feature is beneficial when performing densitometric analysis of silver stained gels.

Features:

• Sensitive—detect proteins at 0.25 ng; equal or better sensitivity than monochromatic metallic silver stains from other suppliers
• Clear background—method produces intensely-stained protein gels with very low background color, shading or anomalies
• Robust performance—reagents and protocol are formulated to yield excellent staining results amidst even wide-ranging differences in user technique
• Rapid, flexible protocol—approx. 1.5 hours are required to complete the default procedure
• Gel-compatible—staining performance is very good with a variety of commercial precast mini-gels and SDS-PAGE buffer-systems
• Workflow-compatible—mild chemical formulation ensures compatibility with mass spectrometry, sequencing and other downstream procedures that depend on destaining and protein recovery