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Thermo Scientific™ SuperSignal™ West Dura Extended Duration Substrate
SuperSignal West Dura Extended Duration Substrate is a luminol-based enhanced chemiluminescence (ECL) HRP substrate with stable light output for mid-femtogram-level detection with CCD-based imagers.
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A reliable product.
Application Area:
Western blotting
When ECL doesn't work, I develop with dura. I don't have background problems ever using dura. If transfer and antibody are good I get a nice signal.
Review Date: 28 Jun 2018 | Thermo Fisher Scientific
Features:
- ECL—enhanced chemiluminescent substrate with high sensitivity and long signal duration for horseradish peroxidase (HRP)
- 24-hour signal duration—light emission is stable for 10 times longer than with typical ECL substrates; acquire multiple exposures to obtain publication-quality blot images
- Great sensitivity—provides detection of mid-femtogram levels of target proteins
- High intensity—immediate, stable signal generation provides easy detection via film or cooled CCD imaging systems
- Stable reagent—24-hour working solution stability; 1-year kit stability at room temperature
- Less antibody usage—works best with dilute antibody concentrations
