Thermo Scientific Verso 1-step qRT-PCR SYBR Green Kits
Thermo Scientific Verso 1-step RT-qPCR Kits for SYBR Green chemistry offer a quick and simple way to detect mRNA. The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures. An optional RT Enhancer is also provided and can be used during the RT step to remove any DNA contamination, eliminating the need for DNase I trea…
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Thermo Scientific Verso 1-step RT-qPCR Kits for SYBR Green chemistry offer a quick and simple way to detect mRNA. The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures. An optional RT Enhancer is also provided and can be used during the RT step to remove any DNA contamination, eliminating the need for DNase I treatment. All Verso 1-step RT-qPCR kits incorporate an inert dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.
Highlights
• Eliminate the need for DNase treatments – RT Enhancer (optional) prevents gDNA carry-over
• RT and qPCR steps performed in single tube – convenience and reduced contamination risk
• Use of the entire RT reaction as template for qPCR – improved sensitivity
• Verso RT enzyme is highly sensitive with broad dynamic range – accurate detection of RNA from 1 pg-1 ug
• Verso RT enzyme has a wide working temperature range (42 to 57°C) – improved success with GC-rich and other difficult templates
• Contains an inert blue dye for ease of pipetting during reaction setup
In a 1-step approach to RT-qPCR, both the RT step and qPCR steps are performed in a single tube (1-step RT-qPCR). Adopting a 1-step approach is ideal for primer sequence optimization and collecting comparative data in experiments that do not require subsequent cDNA storage. The approach minimizes the risk of contamination, and improvements in reproducibility may be observed as a result of reduced sample handling.
Verso 1-Step RT-qPCR Kits for SYBR Green chemistry are compatible with all major real-time thermal cyclers.
Applications
• Gene expression analysis
• RNAi validation
• Microarray validation
• Pathogen detection
• Genetic testing
• Disease research
Includes
• Verso Enzyme Mix
• 1-step qPCR SYBR Mix (includes Thermo-Start Taq DNA Polymerase, RT buffer, PCR buffer containing SYBR Green, dNTPs, MgCl2, inert blue dye, and ROX reference dye where not provided as separate vial)
• RT Enhancer
• MgCl2
The product is available supplemented with high ROX reference dye concentration (AB-4105), low ROX concentration (4106), Fluorescein (AB-4107), or with a separate vial of ROX (AB-4104).
