Transcreener® AMP²/GMP² Phosphodiesterase Assays
The Transcreener® AMP²/GMP² Phosphodiesterase Assay Kit directly detects AMP or GMP produced by PDEs, ligases, synthetases, ENPP1, and related enzymes using a mix‑and‑read FP or TR‑FRET format—no coupling steps needed. Compatible with 1–1000 µM native substrates, it delivers excellent HTS performance (Z’ ≥ 0.7, ≥100 mP shift) in a one-step, far‑red fluorescence assay.
FP AMP/GMP Phosphodiesterase
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Environmental Assesment
The Transcreener AMP/GMP is very easy to use and is very sensitive. In the practical lab, it just take about 1 uM in a UV-based enzyme assay. This product is very useful for environmental assessment.
Review Date: 10 Mar 2014 | BellBrook Labs
Drug Development
The Transcreener AMP/GMP is extremely straightforward to use and is very sensitive. In a UV-based enzyme assay, I needed 1 uM enzyme to see a decent signal; however using the Transcreener AMP assay I only need 10 nM. Also, BellBrook Labs have been very helpful with assay development. Their representative (Meera) has been very willing to quickly help me get my assay up and running. Thank you Meera!
Review Date: 25 Feb 2014 | BellBrook Labs
The Transcreener® AMP²/GMP² Assay provides a direct, mix-and-read method to measure AMP or GMP, the universal products of phosphodiesterase (PDE) and other nucleotide-processing enzymes. Designed for high-throughput screening (HTS), it eliminates coupling steps and reduces assay interference, delivering robust, reproducible results.
Key Features & Benefits
- Direct Detection – Measures AMP or GMP directly with highly selective antibodies, avoiding coupling enzymes or secondary reactions.
- Universal Utility – Works with PDEs as well as ligases, synthetases, ubiquitin-related enzymes, and other nucleotide-dependent enzymes.
- Flexible Detection Modes – Available in Fluorescence Polarization (FP) and TR-FRET formats.
- Far-Red Tracers – Reduce compound interference and improve signal clarity in screening libraries.
- Wide Substrate Range – Compatible with native substrate concentrations from 1–1000 µM.
- HTS Proven – Validated in 96-, 384-, and 1536-well plate formats with excellent assay performance (Z’ ≥ 0.7 and ≥100 mP shifts).
- Automation Ready – Homogeneous mix-and-read format and stable signal (≥8 hours) streamline automated workflows.
Applications
- High-throughput screening of PDE inhibitors
- Mechanism of action and selectivity profiling
- Hit-to-lead optimization with reduced false positives
- Enzyme activity studies across diverse AMP/GMP-generating enzymes
