VIA Extractor™ tissue disaggregator

The VIA Extractor™* tissue disaggregator disaggregates solid tissue into viable single cells. For use in high-throughput omics and flow cytometry research, the closed system uses mild processing to optimize yield and preserve content relative to the parent sample.

• Mild processing optimizes cell viability and yield and preserves content relative to the parent sample.
• Standardized, automated process reduces sample-to-sample variation.
• Disaggregates solid tissue to single cell suspension in as little as ten minutes.
• Adjust the processing speed, temperature and time settings using the VIA Freeze™ Uno controlled-rate freezer to optimize results dependent on sample type and size.
• Process up to three samples in parallel in single-use pouches, minimizing the risk of contamination.

Recent technological advancements have made single-cell omics (genomics, proteomics, metabolomics) an increasingly powerful tool for diagnosis, treatment response prediction and treatment selection for cancer. The process begins with disaggregation of a fresh or frozen sample into a single-cell suspension, followed by preparation of a single-cell next-generation sequencing (NGS) library, then data analysis. The current techniques for sample disaggregation into a single cell suspension commonly involve manual mechanical or enzymatic disruption and can result in poor yields and viability, making them unsuitable for library preparation. The VIA Extractor™ tissue disaggregator overcomes these challenges with semi-automated, gentle processing that optimizes cell viability and preserves the cell content relative to the parent sample.

For omics applications, you will require the following items:

• VIA Extractor™ tissue disaggregator: Device for disaggregation of human and animal solid tissue or tumors.
• VIA Freeze™ Uno controlled-rate freezer: A liquid nitrogen-free controlled-rate freezer combining customizable freezing profiles, electronic control systems, and a conduction cooling method to optimize GMP-compliant cryopreservation. Further details can be found here.
• Omics clamp: Provides a secure seal after sample insertion and supports the bag during tissue dissociation.
• Omics pouch: Single-use, multi-compartment pouch with tubing to allow the addition of digestive enzyme solutions.
• Omics applicator: Open-bore sample insertion syringe to aid insertion of tissue sample into Omics pouch.

To get started, you will also need:

• Forceps or tweezers
• Luer-lock 5 mL syringe
• Heat sealer

Please note that the VIA Extractor™ tissue disaggregator needs to be placed into the VIA Freeze™ Uno controlled-rate freezer to operate. If you are not in possession of a VIA Freeze™ Uno controlled-rate freezer, the Omics bundle should be ordered. The Omics bundle includes the VIA Extractor™ tissue disaggregator, the VIA Freeze™ Uno controlled-rate freezer and the Omics clamp.

* For research use only (RUO). Not for diagnostic use.