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ZymoBIOMICS Fecal Reference with TruMatrix™ Technology

The ZymoBIOMICS Fecal Reference with TruMatrix™ Technology is a microbial reference material composed of stool from healthy donors for quality control, process validation, assay development, and proficiency testing. 

Zymo Research

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Average Rating 4.3

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It is a good product to have as a benchmark for our gut microbiome analysis.

 

Average Rating 4.3

Application Area:

NGS of human fecal microbiome

It is decently easy to use. We have used it multiple times and got consistent results. However, I could only find the expected composition at phylum level in the datasheet. Hopefully the composition information of other taxonomic ranks such as order, family and genus could be released to the users. In general, it is a good product to have as a benchmark for our gut microbiome analysis.

Review Date: 20 Jul 2021 | Zymo Research

The ZymoBIOMICS Fecal Reference with TruMatrix™ Technology is a microbial reference material composed of stool from healthy donors for quality control, process validation, assay development, and proficiency testing. The fecal reference was homogenized in one large batch to produce consistency in every vial, eliminating typical lot-to-lot variability. With over 2 million preps available, this reference is intended to be used as a control for workflow performance, as well as comparing workflow consistency across replicates, studies, and laboratories. To facilitate methods comparison, a platform is in development to enable users to compare characterization data against applied methodology.

  • Biosafety: This product contains no biohazard as potential pathogens have been fully inactivated.
  • Characterization: Links to 16S, metagenomic, and metatranscriptomic profiling are available in Appendix A of the protocol.
  • Concentration: 1mL of the product contains about 10 mg feces (wet weight).
  • Stability: Stored in DNA/RNA Shield, which preserves the microbial profile and protects against degradation from freeze-thaw cycles.
  • Total DNA Content: 1mL of the product contains about 6 µg DNA.
  • Total RNA Content: 1mL of the product contains about 11 µg RNA.

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