ZymoBIOMICS™ Microbial Community Standard
Assess bias and errors in NGS-based microbial composition profiling workflows
ZymoBIOMICS Community Standard
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Great choice
Microbiome
Excelent possibility to choose any standard for microbiome research.
Review Date: 6 Jul 2022 | Zymo Research
Great community standard
NGS
ZymoBIOMICS Microbial Community Standard is a great product. We use it as our contrived sample in our NGS workflows. I love the reproducibility of the results with this product. We even recently used the standard to evaluate DNA extraction methods because the standard has so many microbes with varying cell wall recalcitrance.
Review Date: 17 Jun 2022 | Zymo Research
Great product
NGS
Zymo's Community Standard is a great product for NGS solutions! It is easy to use and provides a robust community sample for sequencing controls.
Review Date: 16 Jun 2022 | Zymo Research
Excellent as a quality control for DNA extraction for NGS applications
Long read sequencing control
The product is easy to use and gave results as expected with the isolates as described. It is very useful as a control for validating extraction methods for genomic DNA from microbial community samples.
Review Date: 20 May 2022 | Zymo Research
Indispensable to check your DNA extraction method
Check efficiency of different DNA extraction methods
We used the ZymoBIOMICS™ Microbial Community Standard to test the efficiency of different DNA extraction methods. The product is very easy to use with a clear protocol. Using this standard, we were able to select the best DNA extraction method for microbial communities in different environments.
Review Date: 29 Mar 2022 | Zymo Research
Essential kit for metagenomics
Animal health metagenomics
Thank you Zymo for this product - would not be possible to benchmark our workflows without this standard.
Review Date: 16 Dec 2021 | Zymo Research
Lab essential.
Microbiome
Community standards are indispensable! No labs should be doing microbiome research without community standards.
Review Date: 15 Dec 2021 | Zymo Research
Exceptional product, reliably of high-quality!
Microbiology
The ZymoBIOMICS Microbial Community Standard is a reliable resource that I have used to standardize microbial molecular biology protocols as well as benchmark bioinformatics tools. This standard has allowed colleagues in my laboratory in early levels of training to safely be introduced to specific techniques, as well as other members with more experience to develop and test their own new protocols. I'd recommend the ZymoBIOMICS Microbial Community Standard fo any laboratory in need of a standardized mock community for molecular biology and bioinformatics applications.
Review Date: 6 Jun 2021 | Zymo Research
Is the best microbial community standard I have used.
Microbiome studies
The Zymobiomics Microbial Community standard is the go-to positive control for all the microbiome studies we do in our lab, based on 16S rRNA gene amplicon sequencing and metagenome sequencing. It consists of good combination of gram positive and negative bacteria, and fungal communities with known relative abundance values. Hence it is ideal to be used as a microbial community standard in experiments that depend on the efficacy of the DNA extraction methodology for reproducible results. A single vial of 750µl (which costs around $300) can be used on 10 different DNA extractions due to its low starting material requirement (75µl). Hence, it gives you high value for money and ease of use. Furthermore, it carries good documentation that clearly indicates what should be the expected relative abundances of the standard microbial communities, for microbial community reconstruction done using it. Thus making it easy to compare results, and pinpoint where certain biases may lie.
Review Date: 16 Mar 2021 | Zymo Research
Great product that is highly reliable and relevant.
Standardization of testing
This product is reliable and of high quality. Batch variation is well controlled. Good mix of organisms mean it suits a variety of applications.
Review Date: 16 Mar 2021 | Zymo Research
ZymoBIOMICS™ Microbial Community Standard is a mock microbial community consisting of eight bacterial and two fungal strains. It includes three easy-to-lyse Gram-negative bacteria (e.g. Escherichia coli ), five tough-to-lyse Gram -positive bacteria (e.g. Listeria monocytogenes ), and two tough-to-lyse yeasts (e.g. Saccharomyces cerevisiae ). Seven of these strains are known human pathogens and have been fully inactivated with DNA/RNA Shield™. The GC content1of the contained genomes covers a range from 15% to 85%. It was constructed by pooling pure cultures of the ten microbial strains. The cells and DNA content of each pure culture were quantified before pooling. Cultures were mixed to a predeterminedcomposition. The actual microbial composition was measured using next-generation sequencing techniques after extracting the genomic DNA with the ZymoBIOMICS™ DNA Mini kit.
