Eclipse™ Flow Field-Flow Fractionation System
Versatile separations of polymers, proteins, colloids, and nanoparticles.
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Unsurpassed quality and support.
Analysis of intermediate and final vaccine products like Viruses, OMV's, proteins, polysaccharides a
The quality is of the highest standard. The support we get is unsurpassed, and fails to compare to any other company. We now see a welcomed trend towards GMP/CFR21 Part11 compliant, which will help us to incorporate the Wyatt instrumentation in our production processes. Especially for our outer membrane vesicles and conjugate vaccine development there is no other instrumentation that could help us to develop such vaccines to such high standard.
Review Date: 7 Jul 2021 | Waters | Wyatt Technology
Great instrument that has significantly improved our research capabilities!
Characterization of antibodies, nanoparticles and extracellular vesicles
We've had the Eclipse AF4 flow field flow fractionation system in our research and development lab for over a year now. The system itself is very easy to use, especially if you have a basic understanding of HPLC and/or SEC. We have been able to obtain successful separation of samples containing both digested and undigested antibodies, and isolation of nanoparticles from multiple supernatant components. Wyatt customer service is great - every time we have run into an issue, we have gotten a response from support in less than 24 hours and they have always been able to solve our problem. The instrument is very expensive, but it is highly versatile and has become a key piece of instrumentation in our lab.
Review Date: 1 Jun 2020 | Waters | Wyatt Technology
It is our view that the Wyatt Eclipse AF4 will replace GPC as the industry standard.
Molar mass, size and shape information for water soluble polymers, proteins and particles
Field flow fractionation is a very difficult technique to master but the Wyatt Eclipse AF4 unit with included Scout predictive software, in addition to their 24/7 customer service, made method development and analyte characterization very achievable and enjoyable. Using one single instrument we have managed to separate and characterize polyelectrolytes with small radii from several nm up to hundreds of nm long rod like cellulose nanocrystals. The versatility and capability of this instrument is incredible and Wyatt's field reps must be credited for helping us realize the full potential of what AF4 can do.
Review Date: 27 May 2020 | Waters | Wyatt Technology
Wyatt Europe is the best partner for all service. Interesting webinars and exceptional customer service.
Analyzing NP-particle interactions with organic colloids in environmental systems
With Wyatt Europe, I have a really good partner in after sales and application development/method development. The system is very flexible and with the service contract I always can count on immediate remote-help. Although, I am not very happy with the dictated combination of hardware and software developments meaning that I am somewhat dependend on expensive software-changes for optimal use of the hardware, and respective bug-fixes. As a university research institute we don't have the money to change working hardware, like in industrial settings. Wyatt US only provides online help if the instrumentation is not older than 10years, which means the possibility to satisfy customers like us is only possible by making a lot of effort. The free webinars offered to us during COVID19 were more than helpful, including the user seminars in the last weeks.
Review Date: 27 May 2020 | Waters | Wyatt Technology
Nice and easy to use AF4 system.
ATMP, Peptides and Antibodies
Nice and easy to use AF4 system. For an AF4 system it is amazingly robust and can be applied to a wide range of applications. The option to use a frit-inlet channel is useful to test for focus stress induced changes of your material.
Review Date: 17 Apr 2020 | Waters | Wyatt Technology
Great system, must-have for high IF paper.
Exosome fractionation
The system works well for the purpose of study which is separation of exosome based on size. Although setup and optimization of instrument are quite complicated, the support staff are well-trained and professional. I usually get replies to emails email within 1-3 working days.
Review Date: 24 Dec 2019 | Waters | Wyatt Technology
AF4 has great potential to fractionationate without the adverse effects of LC/SEC.
Influenza vaccines, proteins, lipid nanoparticles,
The instrument is designed well for ease of use in batch or being modular for online mode. However, the software needs to be well integrated so that it is a more cohesive software package where all of its capabilities are centralized into one package. As a result the software has a steep learning curve. Also, the AF4 channel design can definitly be improved with torqued latches so that assembly can be done more simplistically by flipping latches instead of manually by hand with a torque wrench.
Review Date: 28 Aug 2019 | Waters | Wyatt Technology
Easy to use
Nanoparticle characterization
We used Wyatt Eclipse AF4 coupled with online MALS and DLS for liposome, nanoparticle, and other formulation characterization. It is easy to use, and is able to produce high quality results.
Review Date: 29 Jan 2018 | Waters | Wyatt Technology
This instrument has proved invaluable for the analysis of very difficult samples.
polymers, nanoparticles
This system is able to separate ultra high molecular weight polymers as well as highly branched polymers. Additionally, we are able to determine the radius of different sized nanoparticles and colloids.
Review Date: 20 Jul 2017 | Waters | Wyatt Technology
I had good results
macromolecules
Asymmetrical flow field-flow fractionation when connected to suitable detector such as multi-angle light scattering (MALS) has shown to be a great technique for the analysis molar mass and root mean square of many food macromolecules. The results are reproducible and with good quality
Review Date: 24 Jun 2016 | Waters | Wyatt Technology
The Eclipse™ solves many difficult separation, fractionation and sizing tasks for macromolecules, nanoparticles and colloids by field-flow fractionation (FFF). Combined with Wyatt’s DAWN™ or miniDAWN™ multi-angle light scattering (MALS) detector and Optilab™ refractive index detector, the Eclipse offers a uniquely versatile FFF-MALS solution providing characterization of absolute molar mass, size, particle concentration, conformation and encapsulation.
Features:
- Robust separation of macromolecules and nanoparticles from 1 nm - 1000 nm with excellent resolution. Typical analysis times are 20 - 60 minutes
- Characterize proteins, polymers, viruses and viral vectors, exosomes, drug- and gene-delivery nanoparticles, environmental tracers and nano-emulsions
- No stationary phase: open-channel separation with minimal shear and surface interactions
- Versatile choice of separation channels, permitting sample loads from nanograms to milligrams, including semi-preparative FFF
- Separation methods to enhance resolution or size range are optimized simply by adjusting flow ratios. VISION™ software provides computer-aided method development to save time and boost productivity.
- The system makes use of the highest quality pumps, autosamplers, UV detectors and fraction collectors from Agilent, all orchestrated by VISION software. Optional automated switching between FFF channel and size-exclusion column.
- Fractions can be collected and used for off-line analysis (with electron microscopy, mass spectrometry, etc.), or couple to ICP-MS for elemental analysis. Optional Dilution Control Module enriches fractions.
- Intelligent user interface and software eliminate the need for expert operators
- Wyatt provides extensive options for training and support.
Applications:
- High-resolution molar mass and size distributions of proteins, soluble and insoluble aggregates, natural and synthetic polymers, polymersomes, exosomes, liposomes, viruses, virus-like particles, metallic nanoparticles, quantum dots, drug-delivery and diagnostic nanoparticles, emulsions and many more
- Analysis of fragile macromolecules or particles that are sheared on a size-exclusion column, sticky proteins that adhere to the stationary phase, or large, heavily-branched polymers that elute abnormally from GPC columns
- Separation and characterization of trace nanoparticles from environmental matrices such as soil or seawater
- Detailed size-zeta potential distributions of nanoparticles
- Semi-preparative separations
- AF4 is recognized by the FDA and other regulatory agencies as an orthogonal technique to size-exclusion chromatography for analysis of biopharmaceutical aggregation.
