The AdEasy system saves you a month of work over traditional methods by producing the recombinant adenoviral plasmid by homologous recombination in E. coli. The AdEasy XL system includes BJ5183 cells pre-transformed with the pAdEasy-1 plasmid, this feature dramatically decreases background caused by the non-recombinant shuttle plasmid.
The AdEasy system saves you a month of work over traditional methods by producing the recombinant adenoviral plasmid by homologous recombination in E. coli. The AdEasy XL system includes BJ5183 cells pre-transformed with the pAdEasy-1 plasmid, this feature dramatically decreases background caused by the non-recombinant shuttle plasmid.
BL21-Gold competent cells feature the high transformation efficiency phenotype (Hte) and have the gene encoding endonuclease I (endA) inactivated.
BL21-Gold competent cells feature the high transformation efficiency phenotype (Hte) and have the gene encoding endonuclease I (endA) inactivated.
BL21-Gold competent cells feature the high transformation efficiency phenotype (Hte) and have the gene encoding endonuclease I (endA) inactivated.
BL21-CodonPlus strains are engineered to contain extra copies of genes that encode the tRNAs that most frequently limit translation of heterologous proteins in E. coli. They are ideal for difficult protein expression, especially when codon bias is a problem.
BL21-CodonPlus strains are engineered to contain extra copies of genes that encode the tRNAs that most frequently limit translation of heterologous proteins in E. coli. They are ideal for difficult protein expression, especially when codon bias is a problem.
L21-CodonPlus strains are engineered to contain extra copies of genes that encode the tRNAs that most frequently limit translation of heterologous proteins in E. coli. They are ideal for difficult protein expression, especially when codon bias is a problem.
BL21-CodonPlus strains are engineered to contain extra copies of genes that encode the tRNAs that most frequently limit translation of heterologous proteins in E. coli . They are ideal for difficult protein expression, especially when codon bias is a problem.
BL21-CodonPlus strains are engineered to contain extra copies of genes that encode the tRNAs that most frequently limit translation of heterologous proteins in E. coli. They are ideal for difficult protein expression, especially when codon bias is a problem.
BL21-CodonPlus strains are engineered to contain extra copies of genes that encode the tRNAs that most frequently limit translation of heterologous proteins in E. coli. They are ideal for difficult protein expression, especially when codon bias is a problem.
The basic BL21 strain does not contain the T7 RNA polymerase gene and can be used with non-T7 RNA polymerase protein expression systems.
The basic BL21 strain does not contain the T7 RNA polymerase gene and can be used with non-T7 RNA polymerase protein expression systems.
The basic BL21 strain does not contain the T7 RNA polymerase gene and can be used with non-T7 RNA polymerase protein expression systems.
Replicating eukaryotic DNA in prokaryotic cells can be problematic. Particular eukaryotic genes may contain inverted repeats or secondary structures, such as Z-DNA, that can be rearranged or deleted by E. coli DNA repair systems. The SURE competent cells are deficient in the E. coli genes involved in the rearrangement and deletion of DNA, thus improving cloning efficiencies of DNA containing irregular structures in prokaryoti…
These competent cells offer the highest transformation efficiencies available, enabling dramatically improved transformation efficiencies when cloning large plasmids and ligated DNA.
This collection of competent cells provides customers with classic E. coli strains that have been engineered to become competent for use in cloning experiments.
This collection of competent cells provides customers with classic E. coli strains that have been engineered to become competent for use in cloning experiments.
Agilent offers the SCS110 and JM110 strains, which lack both dam and dcm activity. DNA propagated in these strains can be digested by methylation-sensitive enzymes such as Xba I, Cla I and EcoR II. SCS110 is an endA– derivative of the JM110 strain.
Allows in vivo excision of cloned fragments in the phagemid vector Screening with both antibodies and nucleic acid probes Each premade library comes with necessary strains and helper phage
This collection of competent cells provides customers with classic E. coli strains that have been engineered to become competent for use in cloning experiments.
The pCMV-Script PCR cloning kit is a polymerase chain reaction (PCR) cloning method that can be performed in 1-hour without adding bases to the primers. The kit permits the efficient cloning of PCR fragments with a high yield and a low rate of false positives. In the ligation reaction, PCR products are incubated with the predigested pCMV-Script mammalian expression vector, the restriction enzyme Srf I, and T4 DNA ligase. Cl…
BL21-Gold competent cells feature the high transformation efficiency phenotype (Hte) and have the gene encoding endonuclease I (endA) inactivated.
The XL1-Blue strains are an all-prupose line of competent cells that are ideal for routine cloning needs.
Most E. coli hosts contain both DNA adenine methylation (dam) and DNA cytosine methylation (dcm) genes. These genes code for proteins that methylate specific sequences when DNA is propagated, making subsequent digestion with methylation-sensitive restriction enzymes impossible. Agilent offers the SCS110 and JM110 strains, which lack both dam and dcm activity.
