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Products, services, reviews and techniques used in the identification and validation of the disease-causing target genes and proteins.

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Endothelial Cell Growth Supplement

Merck

ECGS is mitogenic for many types of cells under reduced or serum-free conditions, such as mammalian, avian and human endothelial cells, smooth muscle cells, keratinocytes, melanocytes and hybridomas; ECGS often fully substitutes for feeder layers in culture of fastidious cells.

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Fibroblast Growth Factor basic, human recombinant, animal-free

Merck

Fibroblast Growth Factor-basic (bFGF) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. bFGF also exerts a potent angiogenic activity in vivo. Human bFGF is a 17.2 kDa protein containing 154 amino acid residues. Xu, et al. demonstrated that bFGF synergizes with the BMP antagonist noggin to sustain undifferentiated proli…

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Mammalian Cell Transfection Kit

Merck

The Mammalian Cell Transfection Kit is an optimized system suitable for the introduction of DNA into mammalian cells. DNA is introduced to the cells as a calcium phosphate precipitate. Transfected cells integrate and express the exogenous DNA at a higher efficiency, producing stable clones of the specifically altered genotype and phenotype. This system has been used to introduce various plasmids into a broad range of cell line…

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Enzyme Free Cell Dissociation Solution Hank's Based (1X), liquid

Merck

Enzyme-Free Cell Dissociation Solution is a Hank's based formulation of chelating agents and agents to stabilize their activity on cells. This reagent does NOT contain porcine trypsin or any other protein. It works significantly faster than 0.5mM EDTA. In fact, it works as quickly as trypsin on most cells. Most importantly, it preserves the structural and functional integrity of cell surface proteins and does not have the cyto…

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Enzyme Free Cell Dissociation Solution PBS Based (1X), liquid

Merck

Enzyme-Free Cell Dissociation Solution is a PBS based formulation of chelating agents and agents to stabilize their activity on cells. It works significantly faster than 0.5mM EDTA. In fact, it works as quickly as trypsin on most cells. Most importantly, it preserves the structural and functional integrity of cell surface proteins and does not have the cytotoxic effects sometimes associated with 0.5 mM EDTA. It can be used to…

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ENStem-A™ Neural Expansion Medium

Merck

ENStem-A Neural Expansion Medium is a defined serum-free formulation that has been optimized for the culture and expansion of ENStem-A Human Neural Progenitor Cells. When used in conjunction with L-Glutamine (not provided) and FGF-2 (provided), the expansion medium will allow for the maintenance and proliferation of ENStem-ATM Human Neural Progenitor Cells.

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ReNcell NSC Maintenance Media

Merck

ReNcell NSC Maintenance Medium is a defined serum-free, growth factor-free medium that has been optimized for the growth and in vitro differentiation of ReNcell immortalized human neural progenitor cells. When used in conjunction with FGF and EGF, the maintenance medium will allow for the proliferation of ReNcell immortalized VM and CX neural stem cells. Withdrawal of the growth factors from ReNcell NSC Maintenance Medium will…

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ReNcell Neural Stem Cell Freezing Medium

Merck

ReNcell Neural Stem Cell Freezing Medium is qualified for use with ReNcell immortalized human neural progenitor cell lines, CX and VM cultured in serum-free conditions with ReNcell NSC Maintenance Medium. The optimized formulation allows for consistent cryopreservation and high viability upon thawing and plating.

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