Merck Products & Reviews

Chemicon's mGlu2 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of mGlu2 interactions with its ligands. The cell line exhibits a calcium response with EC50s of 0.51uM, 5.6uM, and 8.3uM for DCG IV, (2R4R) APDC, and glutamate. The membrane preparations exhibit EC50s of of 0.…

EMD Millipore's cloned human GLP-1 -expressing cell line is made in the Chem-9 host, which supports high levels of recombinant GLP-1 expression on the cell surface and contains high levels of promiscuous G proteins to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between GLP-1 and its ligands.

EMD Millipore’s cloned human GLP-1 Receptor -expressing cell line is made in the CHO host, which supports optimal levels of recombinant GLP-1 Receptor expression for robust agonist-induced cAMP signal. In addition, the cell line contains optimized levels of a proprietary promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists and antagonists…

Chemicon's cloned human SST3-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant SST3 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between the SST3 and its ligands.

Chemicon's cloned human CGRP1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant CGRP1 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between CGRP1 and its ligands.

Chemicon's cloned human S1P1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant S1P1 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between S1P1 and its ligands.

EMD Millipore's S1P1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of S1P1 interaction with its ligands. The cell line exhibits a calcium response with EC50s of 141 μM for Sphingosine 1-phosphate. The membrane preparations exhibit EC50s of 9.5 μM for Sphingosine 1-phospha…

Chemicon's cloned human PK2-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant PK2 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between PK2 and its ligands.

Chemicon's cloned human EP2-expressing cell line is made in the Chem-9 host, which supports high levels of recombinant EP2 expression on the cell surface and contains high levels of the promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between EP2 and its ligands.

EMD Millipore's EP2 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists of EP2. The membrane preparations exhibit a Kd of 14.52 nM for [3H]-PGE2. With 7.5 nM [3H]-PGE2, 10µg/well EP2 Membrane Prep typically yields greater than 3-fold signal-to-backgr…

EMD Millipore’s cloned human Ghrelin Receptor-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant Ghrelin Receptor expression on the cell surface and contains high levels of the promiscuous G protein to enhance coupling of the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists and antagonists at Ghrelin Receptor.

EMD Millipore's Ghrelin Receptor membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of ghrelin interactions and its ligands. The membrane preparations exhibit a Kd of 0.32 nM for [125I]-ghrelin. With 0.1 nM [125I]-ghrelin, 5 μg/well Ghrelin Receptor Membrane Pre…

Chemicon's cloned human S1P4-expressing cell line is made in the Chem-5 host, which supports high levels of recombinant S1P4 expression on the cell surface and contains high levels of the promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between S1P4 and its ligands.

EMD Millipore's cloned human S1P5-expressing cell line is made in the Chem-5 host, which supports high levels of recombinant S1P5 expression on the cell surface and contains high levels of the promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between S1P5 and its ligands.

EMD Millipore's S1P5 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of S1P5 interactions with its ligands. The membrane preparations exhibit EC50s of 2.4 nM for S1P in a GTPγS binding assay.

EMD Millipore’s cloned human GPR92 -expressing cell line is made in the Chem-1 host, which supports high levels of recombinant GPR92 expression on the cell surface and contains high levels of the promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists and antagonists at GPR92.

EMD Millipore’s cloned human GPR91 -expressing cell line is made in the Chem-1 host, which supports high levels of recombinant GPR91 expression on the cell surface and contains high levels of the promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists and antagonists at GPR91.

Chemicon's Wild-Type Chem-1 cell line is a control for Chemicon's GPCR-expressing Chem-1 calcium-optimized cell lines. The Chem-1 cell line is adherent to tissue culture-treated surfaces and contains high levels of the promiscuous G protein Galpha15 to couple many classes of GPCRs to the calcium signaling pathway.

ChIP-Seq facilitates genome-wide profiling of the interactions of transcription factors, modified histones, and other chromatin associated proteins. By combining chromatin immunoprecipitation with next generation sequencing, laboratories can generate high resolution data on protein-DNA interactions across the genome. For laboratories interested in gaining a deeper understanding of epigenetic mechanisms of gene regulation, ChIP…

Introducing Direct Detect™, the first infrared (IR)-based biomolecular quantitation system. Just spot your sample, blank, and read! No sample prep, standard curves, messy cuvettes or liquid waste. With inherent amide bond detection, the Direct Detect™ system distinguishes proteins and peptides from interfering sample components, providing more accurate results without the pitfalls of colorimetric assays. Technology: FTIR Spe…

Indispensable for NMR spectroscopy Unlocking the molecular structure of organic molecules is central to understanding how they interact with other molecules. This is especially critical in genomics, proteomics and drug discovery research. The Nuclear Magnetic Resonance (NMR) technique allows scientists to “see” molecular structure and dynamics. MagniSolv™ deuterated solvents are a key tool for the NMR technique. MagniSolv™ deu…

The PureGenome™ High Efficiency Next Generation Sequencing (NGS) Library Preparation Reagents are uniquely optimized as a one-stop solution for NGS Library Preparation. Validated for sequencing compatibility with Illumina® instruments; three sets of reagents are offered each having a unique functionality in the library preparation work flow. The PureGenome NGS Library DNA Modifier, when purchased with the PureGenome NGS Librar…

The PureGenome™ High Efficiency Next Generation Sequencing (NGS) Library Preparation Reagents are uniquely optimized as a one-stop solution for NGS Library Preparation. Validated for sequencing compatibility with Illumina® instruments; three sets of reagents are offered each having a unique functionality in the library preparation work flow. The PureGenome NGS Library DNA Modifier, when purchased with the PureGenome NGS Librar…

The PureGenome™ High Efficiency Next Generation Sequencing (NGS) Library Preparation Reagents are uniquely optimized as a one-stop solution for NGS Library Preparation. Validated for sequencing compatibility with Illumina® instruments; three sets of reagents are offered each having a unique functionality in the library preparation work flow. The PureGenome NGS Library DNA Modifier, when purchased with the PureGenome NGS Librar…

The quality of sequence obtained from Next Generation sequencing platforms such as Illumina, Inc. requires the accurate determination of input library DNA. Loading too much DNA can lead to overlapping clusters and loss of read counts whereas too little input can lead to poor read lengths and lower sequence output. The PureGenome™ NGS Library Validator Kit measures the absolute amount of adapter-ligated, sequence ready DNA in I…