The 10x Compatible Human CRISPRi Kinase Phosphatase Drug Targets Kit (Druggable Genome) contains one sub-pool of the top two ranked sgRNAs per gene for increased sensitivity and includes non-targeting controls built-in.
The Human CRISPR activation library (Calabrese) activates over 18,000 human genes and is used for genome-wide activation screening. The library is designed to be compact and efficient to maximize screening efficiency and performance, as published by Sanson, K.R., et al. Nat Commun 9, 5416 (2018).
The mouse CRISPR activation library (Caprano) activates over 22,000 mouse genes and is used for genome-wide activation screening.
The control kit includes validated positive control targeting RAB1A and non-targeting control for assay set-up and a UCOE KRAB-dCas9 for consistent effector expression across a wide variety of cell lines. The kit is ideal for setting up the screen assay and optimization before using the CRISPRi library pools.
The 10X CRISPRi Feature Barcode Optimization kit is ideally suited for setting up the screen assay and if desired capture sequence optimization before using the Custom 10x Compatible CRISPRi library pools.
The MISSION pLKO.1-puro Control Vector is a lentivirus plasmid vector. The vector does not contain an shRNA insert and is useful as a negative control in experiments using the MISSION shRNA library clones.
The MISSION pLKO.1-puro Control Vector is a lentivirus plasmid vector. The vector does not contain an shRNA insert and is useful as a negative control in experiments using the MISSION shRNA library clones.
Developed at the Broad Institute, the MISSION ® TRC3 human whole genome pooled ORF library consists of over 17,000 unique ORF clones, encoding for over 14,000 genes used for overexpression screening
The MISSION TRC3 ORF GFP Control Vector is a lentivirus plasmid vector. The vector contains an ORF insert expressing TurboGFP™, and is useful as a positive (or negative control) in experiments using the MISSION TRC3 LentiORF Library Collection in arrayed or pooled format
This pooled library targets the human kinome, a collection of approximately 700 genes commonly associated with development and disease, with an average of nearly 9 unique knockout CRISPR clones per kinase gene. The lentiviral format allows for stable integration, selection, and enrichment of transduced cells
Utilize the power of CRISPR Whole Genome Lentiviral Pools to knockout and screen every gene in the human genome. The Sigma Whole Human Genome Lentiviral CRISPR Pool is provided as 200 ul in 8 x 25 ul aliquots at a minimum titer of 5x108 viral particles/ml via p24
Accomplish highly effective transfection of CRISPR/Cas9 DNA, RNA, Cas9-gRNA RNP (ribonucleoprotein) complexes and siRNA to knock-out, modify or knock-down genes of interest in a wide variety of cell types.
Recombinant Cas9-D10A Nickase protein from Streptococcus pyogenes (~160 KD) is a ready-to-use reagent for genome engineering experiments.
The Sigma lenti CRISPR Non-Targeting Control is a lentiviral plasmid vector, which includes a gRNA sequence that does not target known human, mouse and rat genes. This vector is useful as a negative control in experiments using Sigma CRISPR lentiviral clones.
Human CRISPRi Kinase Phosphatase Drug Targets Kit contains 2 sub-pools of top 5 gRNAs per gene as well as a separate sub-pool of 5 supplemental gRNAs per gene for increased sensitivity and includes non-targeting controls built in.
The Lenti CRISPR Non-Target Control#1 (LV04 vector) is a lentiviral plasmid vector, which includes a gRNA sequence that does not target known human, mouse and rat genes. The DNA format of this control construct permits direct transfection in knockout experiments or use in the viral packaging process (for lentiviral-based knockdown experiments).
The Sigma lenti CRISPR Non-Targeting Control is a lentiviral system, which includes a gRNA sequence that does not target known human, mouse and rat genes. The non-targeting control lentivirus is useful as a negative control in experiments using Sigma CRISPR lentiviral clones.
Pooled CRISPR screening with 10x genomics compatibility for discovery at single cell resolution
Certified reference material, Trace CERT ® , 100 μg/mL each component in methanol, ampule of 1 mL.
Platform can perform automatic analysis of sample's purity, identification and degradation of compounds through calibrated algorithm-based digital references.
For researchers who want to do cell cycle research at their benchtop, Guava Cell Cycle assay can be used for measuring G0/G1, S, and G2/M phase distributions. Based on the established method of whole-cell staining with propidium iodide (PI), Guava's cell cycle assay is easy to learn. Guava Cell Cycle software includes markers that can be set for quick assessment of G0/G1, S, and G2/M phase cell cycle percentages as data is bei…
Apoptosis, or programmed cell death, is a highly regulated pathway that is important in normal developmental processes as well as many diseases. Cells undergoing apoptosis are identifiable by a number of characteristics, including changes in mitochonrial membrane potential, transport of phosphatidylserine (PS) to the membrane surface, activation of caspase proteases, and DNA fragmentation in the nucleus. Guava offers four apop…
Guava Technologies' ViaCount assay is revolutionizing the way cell counting and viability assessment are done in today's laboratories. Fast, automated, and highly reproducible, the Guava ViaCount assay addresses many of the difficulties of conventional cell counting and viability assessment methods. The Guava ViaCount assay provides: Cell counts up to ten times faster than manual counting, in a convenient turnkey assay.…
