Products & Reviews

Pfu DNA Polymerase is a high-fidelity, thermostable enzyme of approximately 90kDa isolated from Pyrococcus furiosus. The enzyme replicates DNA at 75°C, catalyzing the polymerization of nucleotides in the 5´→3´ direction in the presence of magnesium. Pfu DNA Polymerase also possesses 3´→5´ exonuclease (proofreading) activity. Base misincorporations that may occur during polymerization are rapidly excised by this proofreading ac…

New Site-Specific Endoprotease that Targets Proline and Alanine

GoTaq®2-Step RT-qPCR System is a reagent system for quantitative analysis of RNA using a two-step reverse transcription-quantitative PCR (RT-qPCR) method. The first step consists of cDNA synthesis using the GoScript™ Reverse Transcription System. Optimized reagents and ultra-active, GoScript™ Reverse Transcriptase result in high-efficiency, full-length cDNA synthesis from rare and abundant targets with up to 5 µg of RNA inp…

GoTaq® Hot Start Polymerase contains the high-performance GoTaq® DNA Polymerase bound to a proprietary antibody that blocks polymerase activity. The system is supplied with a tube of 25mM MgCl2, allowing optimization of the magnesium concentration in your reactions. It is also supplied with 5X Green GoTaq® Flexi Buffer and 5X Colorless GoTaq® Flexi Buffer. The buffers contain a compound that increases sample density, so tha…

GoTaq® Hot Start Master Mixes are premixed, ready-to-use solutions containing GoTaq® Hot Start Polymerase, magnesium, dNTPs and buffer. Reactions can be set up in less than a minute at room temperature; simply add your template, water and primers. Available with either green or colorless reaction buffers, which also serve as loading buffers, allowing you to go directly from thermal cycler to gel analysis. GoTaq® Hot Start Mast…

The QuantiFluor® dsDNA System contains a fluorescent DNA-binding dye that enables sensitive quantitation of small amounts of double-stranded DNA (dsDNA) in solution.   The quantitation of dsDNA is a very important step in many biological applications, particularly in standard molecular biology techniques. The dye shows minimal binding to single stranded DNA (ssDNA) and RNA, allowing specific quantitation of dsDNA. The…

The PowerPlex® ESX 17 and ESI 17 Fast Systems meet recommendations of the European Network of Forensic Science Institutes (ENFSI) for DNA profile sharing across Europe. They are based on five-color technology and allow co-amplification and detection of D3S1358, D8S1179, D18S51, D21S11, FGA, TH01, vWA, D2S441, D10S1248, D22S1045, D1S1656, D12S391, D2S1338, D16S539, D19S433, SE33 and Amelogenin. These kits are available in…

The resin-based Wizard® Plus Minipreps DNA Purification System provides a simple and reliable method for rapid isolation of plasmid DNA.   When using the standard protocol, the entire miniprep process can be completed in 15 minutes or less, with no organic extractions or ethanol precipitations. Minipreps may be processed individually or in multiples with the Vac-Man® (20-sample capacity, Cat.# A7231) or Vac-Man® Jr. (2-s…

The PureYield™ Plasmid Maxiprep System isolates transfection-quality plasmid DNA. The system provides a rapid method for purification of up to 1mg of plasmid DNA from a 250ml bacterial culture. Plasmid DNA can be purified rapidly with the vacuum protocol, greatly reducing the time spent on purification compared to silica resin or other membrane-column methods. The PureYield™ Plasmid Maxiprep System incorporates a unique End…

Single-Use Ligands for High-Resolution lmaging in Live Cells

The PureYield™ Plasmid Midiprep System is designed to isolate transfection-quality plasmid DNA.   The system provides a rapid method for purification of 100–200μg of plasmid DNA from 50ml bacteria culture. Plasmid DNA can be purified in as little as 30 minutes with the vacuum protocol, greatly reducing the time spent on purification compared to silica resin or other membrane-column methods. An alternative protocol allows…

GoTaq® qPCR Master Mix is a new reagent system for use in real-time quantitative PCR (qPCR). The system contains BRYT™ Green, a new proprietary fluorescent DNA binding dye that exhibits greater fluorescence enhancement upon binding to double-stranded DNA than SYBR® Green I. Combined with the GoTaq® Hot Start Polymerase, optimized buffer and proprietary dye, GoTaq® qPCR Master Mix provides robust real-time PCR with increased re…

The GoScript™ Reverse Transcription System includes a reverse transcriptase and a specialized set of reagents for efficient synthesis of first-strand cDNA optimized for quantitative PCR amplification. GoScript™ Reverse Transcriptase uses M-MLV Reverse Transcriptase and state-of-the-art buffer technology to deliver robust, reliable cDNA synthesis of a full range of rare and abundant transcripts, even in the presence of inhibit…

Brighter signal for earlier quantification of low and high copy RNA targets in a convenient 1-Step RT-qPCR format GoTaq® 1-Step RT-qPCR System is a reagent system that gives a brighter signal for earlier quantification of low and high copy RNA targets, using a one-step reverse transcription-quantitative PCR (RT-qPCR) protocol in a single tube format. The system incorporates BRYT Green® Fluorescent Dye, a novel dye…

The PowerPlex® Y23 System is a 23-loci, 5-color Y-STR multiplex designed for genotyping forensic casework samples, database samples and paternity samples.   The kit contains: all 12 loci in the current PowerPlex® Y System, the additional 5 loci found in AmpFlSTR® Y-filer®, plus 6 new loci.   PowerPlex® Y23 System Benefits: Reveal more Y-STR loci in excess female DNA Cut your amplification time roughly in ha…

The DNA IQ™ System is a DNA isolation system designed specifically for forensic and paternity laboratories. This system employs novel paramagnetic particles to isolate clean DNA for use with short tandem repeat (STR) analysis. The DNA IQ™ System can be used to extract DNA from a variety of sample types, including stains and liquid samples. Protocols for database samples and casework samples are available. The unique DNA IQ™…

The GoTaq® Probe qPCR Master Mix is optimized for quantitative PCR assays in the hydrolysis probe detection format.   It is provided as a ready-to-use, stabilized 2X formulation that includes all components for qPCR (except template, primers and probe). This master mix does not contain a reference dye; however, a separate tube of carboxy-X-rhodamine (CXR) reference dye is included with this system, allowing users to add…

The GoTaq® Probe 2-Step RT-qPCR System is optimized for quantitative PCR assays in the hydrolysis probe detection format. The system protocol facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using integrated components: • GoScript™ Reverse Transcription System • GoTaq® Probe qPCR Master Mix The GoScript™ Reverse Transcription System includes an optimized reaction b…

If there is DNA to detect, the Plexor® HY System will show it. Total human DNA. Total male DNA. The power to see it all in a single assay.   The Plexor® HY System is a real-time PCR assay to determine the concentration of total human DNA and male human DNA simultaneously in one reaction. The kit contains an internal PCR control (IPC) to test for false-negative results that may occur in the presence of PCR inhibitors…

The GoTaq® G2 Hot Start Polymerase and Master Mixes simplify your reaction preparation, bringing you the convenience of room-temperature setup and the specificity of a hot start polymerase.   Ready-to-use Master Mix simplifies reaction setup and allows direct-to-gel loading. The optimized formulation provides amplification of a variety of targets, while eliminating non-specific products, resulting in higher yields and gr…

Diamond™ Nucleic Acid Dye is a safe, sensitive fluorescent dye that binds to single-stranded DNA, double-stranded DNA and RNA, and can be used to stain and visualize nucleic acids in gels.   Diamond™ Nucleic Acid Dye is compatible with denaturing and native agarose and polyacrylamide gels and can be imaged with any standard imaging system. Significantly less mutagenic than traditional ethidium bromide, as proven by Ames…

The BenchTop DNA Markers, supplied in a stabilizing solution of 1X Blue/Orange Loading Dye, come in a variety of different sizes to meet your DNA electrophoresis needs.

High-quality deoxynucleotide triphosphates (dNTPs) are critical for the success of many key procedures such as cDNA synthesis, sequencing and labeling.   Promega dNTPs have greater than 99% triphosphate content and are provided at a concentration of 100mM in water at pH 7.5. dNTPs Features & Benefits:   Dependable - PCR-tested deoxynucleotides ensure optimal performance with all Promega amplification enzyme…

GoTaq® Long PCR Master Mix contains the high-performance GoTaq® Hot Start Polyemrase in a specially formulated mixture with a proprietary thermostable proofreading polyemrase.   This optimized enzyme mixture allows efficient amplification of up to 40kb from lambda DNA or 30kb from human genomic DNA. The presence of a proofreading enzyme to repair DNA mismatches and a highly processive polyemrase allows the polyemras…

The Maxwell® Clinical Sample Concentrator (CSC) automated nucleic acid extraction system is designed for clinical laboratories needing to process multiple samples for critical downstream assays. This easy-to-use, cGMP-compliant system provides consistent, high-quality results with every run, allowing you to manage your workflow and gain efficiency. The purification methods use sample lysis and binding to paramagnetic par…