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The Human Regulatory T cell Staining Kit #3 contains all of the buffers and monoclonal antibodies for CD4, CD25, and Foxp3 necessary to successfully stain and identify regulatory T cells from human peripheral blood cells. The kit contains the following labeled monoclonal antibodies sufficient for 25 tests: eZFluor™ Anti-Human CD4 FITC and CD25 PE Cocktail (clones RPA-T4 and BC96), Anti-Human Foxp3 PE-Cyanine5 (clone PCH101), R…

The Human Regulatory T Cell Whole Blood Staining Kit contains the buffers and monoclonal antibody necessary to successfully stain and identify Foxp3 cells in whole blood samples. The Anti-Human Foxp3 PE (PCH101) monoclonal antibody reacts with the amino terminus of human Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2. Foxp3 is a 49-55 kDa protein and a member of the forkhead/winged-helix family of transcription factors.…

The Mouse Regulatory T cell Staining Kit #1 contains buffers and monoclonal antibodies for CD4, CD25, and Foxp3 necessary to successfully stain and identify mouse regulatory T cells from human peripheral blood cells. The kit contains the following labeled monoclonal antibodies: Anti-Mouse CD4 FITC (RM4-5), Anti-Mouse CD25 APC (PC61.5), Anti-Mouse/Rat Foxp3 PE (FJK-16s), Rat IgG2a K Isotype Control PE, Anti-Mouse CD16/32 Purifi…

The Mouse Regulatory T cell Staining Kit #1 contains buffers and monoclonal antibodies for CD4, CD25, and Foxp3 necessary to successfully stain and identify mouse regulatory T cells from human peripheral blood cells. The kit contains the following labeled monoclonal antibodies: Anti-Mouse CD4 FITC (RM4-5), Anti-Mouse CD25 PE (PC61.5), Anti-Mouse/Rat Foxp3 APC (FJK-16s), Rat IgG2a K Isotype Control APC, Anti-Mouse CD16/32 Purif…

The Mouse Regulatory T cell Staining Kit #1 contains buffers and monoclonal antibodies for CD4, CD25, and Foxp3 necessary to successfully stain and identify mouse regulatory T cells from human peripheral blood cells. The kit contains the following labeled monoclonal antibodies: Anti-Mouse CD4 FITC (RM4-5), Anti-Mouse CD25 PE (PC61.5), Anti-Mouse/Rat Foxp3 PE-Cy5 (FJK-16s), Rat IgG2a K Isotype Control PE-Cy5, Anti-Mouse CD16/32…

JC-1 is a membrane permeable dye widely used for determining mitochondrial membrane potential in flow cytometry and fluorescent microscopy. This dye can selectively enter the mitochondria where it reversibly changes coloras membrane potentials increase (over values of about 80-100 mV). When excited at 488 nm, the emission shift goes from 530 nm (monomeric JC-1) to 590 nm (aggregate JC-1), which are easily detected using filter…

eFluor 514 Calcium Sensor Dye is a membrane-permeable dye used for monitoring changes in intracellular free calcium concentrations in the cell using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. When it enters a cell, cellular esterases cleave the dye, resulting in a membrane-impermeable dye that fluoresces at ~520 nm. eFluor™ 514 Calcium Sensor Dyeis a commonly used dy…

DRAQ5, an anthraquinone dye with high affinity for double-stranded DNA, is a membrane-permeable dye that can label live or fixed/dead cells. The dye is used to distinguish nucleated and non-nucleated cells in flow cytometry, to report nuclear DNA content for ploidy and cell cycle analysis and as a nuclear counterstain nuclei in fluorescent microscopy.DRAQ5 can be excited by wavelengths from 488 to 647 nm. For microscopy/imagin…

The mouse Th17 Cytokine Staining Panel includes all reagents needed for simultaneous flow cytometric detection of all the major cytokines produced by the Th17 lineage: IL-17A, IL-17F, IL-21 and IL-22. An anti-CD4 antibody that can be used after fixation and permeabilization and IC Fixation and Permeabilization Buffers are also included.CD4+ T helper cells are critical mediators of the cellular immune response. Th17 is subset o…

The Human Th17 Cytokine Staining Panel includes all reagents needed for simultaneous flow cytometric detection of all the major cytokines produced by the Th17 lineage: IL-17A, IL-17F, IL-21 and IL-22. An anti-CD4 antibody that can be used after fixation and permeabilization and IC Fixation and Permeabilization Buffers are also included.CD4+ T helper cells are critical mediators of the cellular immune response. Th17 is subset o…

The Cell Stimulation Cocktail (plus protein transport inhibitors) (500X) is a cocktail of phorbol 12-myristate 13-acetate (PMA), ionomycin, brefeldin A and monensin. PMA and ionomycin treatment can induce activation of many cell types to produce cytokines. Brefeldin A and Monensin result in the accumulation of secreted proteins in the endoplasmic reticulum and Golgi apparatus. The Cell Stimulation Cocktail (plus protein transp…

The Lipoploysaccharide (LPS) Solution (500X) is a ready-to-use solution of LPS from Escherichia coli 026:B6 in aqueous solution. LPS is highly immunogenic and strongly activates immune cells bearing the CD14/TLR4/MD2 receptor complex. This reagent is intended for use in in vitro culture of B cells, monocytes, macrophages and other cells that are responsive to LPS.

The Phytohemagglutinin-L (PHA-L) Solution (500X) is a ready-to-use solution of PHA-L in aqueous buffer. PHA is a mixture of lectins isolated from the red kidney bean, Phaseolus vulgaris. PHA-L is the fraction of PHA that exhibits strong leukoagglutinating and mitogenic activity, but very little erythroagglutinating activity. This reagent is intended for use in in vitro activation of human and mouse leukocytes.

The Concanavalin A (Con A) Solution (500X) is a ready-to-use solution of Con A in aqueous buffer. Con A, isolated from the Jack bean, Canavlia ensiformis, is a lectin that binds alpha-D-glucose and alpha-D-mannose moieties found in various glycoproteins, glycolipids, and sugars and is a potent leukocyte mitogen. This reagent is intended for use in in vitro activation of human and mouse leukocytes.

The Protein Transport Inhibitor Cocktail (500X), a cocktail of Brefeldin A and Monensin, is used for assays in which prevention of protein transport to the extracellular space is desired and has been specifically formulated for use as a negative control for the Cell Stimulation Cocktail (plus protein transport inhibitors) (500X) (cat. 00-4975). Addition of this cocktail to cells in culture inhibits the intracellular protein se…

The Foxp3 Transcription Factor Fixation/Permeabilization Concentrate and Diluent solutions are used for staining with antibodies to transcription factors and nuclear proteins, such as Foxp3 and Ki-67, as well as cytokines and chemokines. It should be used in combination with the Permeabilization Buffer (10X) (cat. # 00-8333).

The Foxp3 Transcription Factor Staining Buffer Set has been formulated and optimized for staining with antibodies to transcription factors and nuclear proteins, such as Foxp3 and Ki-67, as well as cytokines and chemokines.

The Super Bright Staining Buffer is a supplement to Flow Cytometry Staining Buffer for immunofluorescent staining protocols of cells in suspension. Super Bright Staining Buffer prevents non-specific polymer interactions when more than one SuperBright conjugated antibody is used in the same experiment, which otherwise could lead to data appearing under-compensated. The buffer is provided in a convenient 5 μL/test format and is…

The 2-component FIX & PERM Cell Permeabilization Kit is used in flow cytometry for fixing and permeabilizing cells in suspension, allowing access of antibody to intracellular targets without sacrificing scatter characteristics.FeaturesFlexible—compatible with multiple sample types and cellular antigensRobust—scatter pattern of cells unaffectedEfficient—simultaneous detection of intracellular and surface markersThe FIX & PERM f…

Cal-Lyse solution is a lysing solution formulated for the lysis of erythrocytes in samples of anti-coagulated human peripheral blood. Cal-Lyse lysing solution can be used as an aid in the enumeration of leukocytes that have been stained with monoclonal antibodies for flow cytometric analysis.

High-Yield Lyse solution, premixed, fixative-free erythrocyte lysing solution, can be used to eliminate red cells from whole blood for flow cytometric analysis with minimal loss of rare blood cell populations. The solution immediately lyses erythrocytes following staining of the blood samples with fluorochrome-conjugated monoclonal antibodies

CellEvent Caspase-3/7 Green Ready Flow Reagent enables the flow cytometric detection of activated caspase-3 and caspase-7 in apoptotic cells. The product includes 2 reagents: the novel fluorogenic substrate CellEvent Caspase-3/7 Green Detection Reagent and SYTOX™ AADvanced™ Dead Cell Stain.FeaturesStable at room temperatureConvenient, ready-to-use format—no need to dilute, weigh, or pipetteExceptionally bright green fluorescen…

The Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay Kit provides a simplified, more robust assay for analyzing DNA replication in proliferating cells as compared to traditional BrdU methods. The Click-iT EdU Flow Cytometry Assay Kits rely on EdU (5-ethynyl-2´-deoxyuridine), a thymidine analog which is incorporated into DNA during active DNA synthesis, which is then detected using click chemistry, a copper catalyzed covalent…

The Click-iT EdU Alexa Fluor 488 Flow Cytometry Assay Kit provides a simplified, more robust assay for analyzing DNA replication in proliferating cells as compared to traditional BrdU methods. The Click-iT EdU Flow Cytometry Assay Kits rely on EdU (5-ethynyl-2´-deoxyuridine), a thymidine analog which is incorporated into DNA during active DNA synthesis, which is then detected using click chemistry, a copper catalyzed covalent…

The Click-iT Plus EdU Pacific Blue Flow Cytometry Assay Kit provides a simplified, more robust assay for analyzing DNA replication in proliferating cells as compared to traditional BrdU methods. The Click-iT EdU Flow Cytometry Assay Kits rely on EdU (5-ethynyl-2´-deoxyuridine), a thymidine analog which is incorporated into DNA during active DNA synthesis, which is then detected using click chemistry, a copper catalyzed covalen…