Separations is a fundamental scientific discipline focused on the isolation and purification of components from mixtures. Through techniques such as chromatography, distillation, and filtration, separations scientists separate substances based on their physical or chemical properties. This field plays a crucial role in various industries, including pharmaceuticals, food and beverage testing, environmental science, materials research, and more. Explore how separations techniques enable precise analysis, quality control, and the development of innovative products.
Alumina, like silica, is an extremely polar sorbent. The alumina surface tends to be slightly more stable under high pH conditions than unfunctionalized silica. The small particle size of the Bond Elut Alumina range ensures high extraction efficiency even when small bed masses are used.
Due to the methyl group and subsequent low carbon load, Bond Elut C1 is the least retentive of all alkyl group bonded phases for nonpolar compounds. However, due to the extensive endcapping of this sorbent to mask polar silanol activity, retention, and elution of polar and multifunctional analytes can still be achieved.
Bond Elut EWP is based upon standard particles size silica but with 500 pores to allow more efficient extraction of large molecules (>15,000 MW), which are typically excluded from standard porosity silica phases.
Bond Elut C18 OH is a nonendcapped version of the octadecyl bonded phases that enables the silanols on the silica surface to be more active. This low-load C18 has well-controlled silanol activity that permits the fractionation of metabolites and enhances retention of basic compounds compared to an endcapped C18. The pore size makes it a good choice for peptide extraction.
Bond Elut C2 is a fairly nonpolar sorbent because of the short chain length of the functional group. C2 is often used during the process of method development if analytes are retained too strongly on a C8 or C18 phase. The polarity of C2 is slightly lower than a cyano phase for polar interactions.
Bond Elut C8 is very similar in property to C18, but is not as retentive for nonpolar compounds due to its shorter hydrocarbon chain and therefore reduced carbon loading. C8 is an excellent replacement for C18 when analytes are too strongly retained for effective elution.
Bond Elut Carbon cartridges are packed with ultrapure graphitized carbon particles that have been optimized for the absorption of pigments in food, fruits and vegetables, and small organic residues in wastewater. The powerful retention mechanisms of these products are appropriate for a broad range of analytes. In addition, careful manufacturing techniques result in lower carbon fines on the wall of the device.
CBA is a midpolarity sorbent and weak cation exchanger (pKa 4.8). It can be used with a wider range of counterions than lower pKa sorbents like SCX, and will demonstrate easier elution of quaternary amine functionalized analytes.
Bond Elut Cellulose columns utilize a pure microgranular cellulose powder that is packed between two, 20 µm polyethylene frits. The cellulose phase is very stable over a wide pH range and contains an extremely low metal content (Fe and CU content less than 5 ppm).
The Bond Elut Certify extraction cartridge utilizes a packed bed consisting of a special, nonpolar C8 sorbent and a strong cation exchanger (SCX). Certify is most commonly used to extract basic (cationic) drugs from urine and blood, but it is also very effective for extracting a wide range of compounds from a diverse number of aqueous matrices.
Bond Elut Certify II was developed specifically for the rapid and effective extraction of acidic drugs and metabolites from urine and other biological matrices. Certify II is a mixed-mode cartridge packed with nonpolar C8 and strong anion-exchange (SAX) sorbent. It has been optimized for acidic drugs, such as 11-nor-delta-9-tetrahydrocannibinol-carboxylic acid, salicylic acid, ibuprofen, acetaminophen, and other compounds tha…
Bond Elut CH is a midpolarity sorbent that exhibits unique selectivities for certain analytes. When employed as a nonpolar sorbent, CH has the approximate polarity of a C2 sorbent. Bond Elut CH is often a good choice when nonpolar sorbents, such as C18, C8, or C2, do not provide the desired selectivity.
A medium polarity sorbent with many uses, Bond Elut Cyano (CN) is ideal for applications in which extremely nonpolar compounds would be irreversibly retained on high carbon load sorbents, such as C8 and C18. This endcapped version of the cyano sorbent is best utilized when extracting analytes from an aqueous matrix.
Bond Elut DEA bears some resemblance to Bond Elut NH2 in its properties, but with a slightly lower capacity as an anion-exchange sorbent. DEA has a moderately nonpolar character due to the alkyl side chains on the amino functionality. These groups still afford a medium level of polarity, higher than C8, but less polar than C2 or CN-E.
Bond Elut Diol resembles unbonded silica as it is known for strong hydrogen bonding with analytes. 20H can also be employed in the nonpolar mode because the hydrocarbon spacer on its functional group provides enough nonpolar character for retention of hydrophobic analytes. Bond Elut Diol is a listed SPE device for the DIN 14333-1 method on benzimidazole fungicides.
Bond Elut ENV, a PS-DVB polymer, is designed for the extraction of polar organic residues. It contains 125 µm spherical particles with a high degree of cross linking, advantageous for high volume, fast flowthrough applications.
Florisil is a magnesia-loaded silica gel. Like silica, it is extremely polar in nature and ideal for the isolation of polar compounds from nonpolar matrices. The larger particle size of the sorbent enables fast flow for large sample volumes and therefore can be an attractive alternative to silica if the sample matrix is particularly viscous.
Bond Elut LMS polymeric sorbent lets you elute without having to add amine modifiers, buffers, or acids. The elimination of secondary interactions means that elution of analytes can be achieved with pure organic solvents or HPLC mobile phase-compatible solvent mixtures of low ionic strength. These characteristics are crucial to allow compatibility with LC/MS or other delicate analytical techniques.
Bond Elut Mycotoxin is a novel sorbent which cleans up food extracts for improved trichothecene and zearalenon analysis. Results are comparable or superior to competing methods, including immunoaffinity columns (IAC) and charcoal/alumina column. The sorbent is a proprietary silica-based ion exchange material.
Bond Elut NEXUS is an ultra-clean polymeric sorbent, which has bimodal porosity and a high surface area. NEXUS offers a nonpolar retention mechanism with no preconditioning required. NEXUS WCX is a weak cation-exchange sorbent that offers extra selectivity for analytes such as quaternary ammonium drugs and anabolic steroids.
Bond Elut NH2 is a weaker anion exchanger than sorbents such as SAX (a quaternary amine sorbent that is always charged), and is therefore a better choice for retention of very strong anions, such as sulfonic acids, which may retain irreversibly on a SAX sorbent. Similar to Diol an SI sorbents, Bond Elut NH2 is excellent for the separation of structural isomers.
Bond Elut PBA is a unique silica SPE sorbent containing a phenylboronic acid functionality that can retain analytes through a reversible covalent bond. This very strong covalent retention mechanism enables high specificity and cleanliness. The boronate group has a strong affinity for cis-diol containing compounds, such as catechols, nucleic acids, some proteins, carbohydrates, and PEG compounds.
Bond Elut PCB is a specially designed sorbent that allows for the facile extraction of polychlorinated biphenyl (PCB) compounds from various matrices. Desired analytes can be loaded and eluted using a simple, single solvent method before analysis by GC/ECD.
Bond Elut PH is a nonpolar bonded silica material, which exhibits a different selectivity to alkyl or aliphatic functionalized phases, such as C8 or cyclohexyl. The electron density present in the aromatic ring affords an enhancement in the retention of conjugated or aromatic ring-containing analytes due to desirable pi-pi interactions.
Bond Elut Plexa PAX sets the new performance standard in analyte cleanup and reproducibility for polar and non-polar acidic analytes. Existing polymeric anion-exchange sorbents can exhibit a broad range of ion exchange capacity from batch to batch. This leads to nonreproducible methods and compromised data.
