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Multiplex protein analysis in human serum and cell culture supernatant

Multiplex protein analysis in murine serum and cell culture supernatant

Multiplex protein analysis in murine serum and cell culture supernatant

Multiplex protein analysis in murine serum and cell culture supernatant

Multiplex protein analysis in murine serum and cell culture supernatant

Multiplex protein analysis in murine serum and cell culture supernatant

Multiplex protein analysis in human aqueous humor, vitreous humor, tears

Multiplex protein analysis in human aqueous humor, vitreous humor, tears

JC-1 is a membrane permeable dye widely used for determining mitochondrial membrane potential in flow cytometry and fluorescent microscopy. This dye can selectively enter the mitochondria where it reversibly changes coloras membrane potentials increase (over values of about 80-100 mV). When excited at 488 nm, the emission shift goes from 530 nm (monomeric JC-1) to 590 nm (aggregate JC-1), which are easily detected using filter…

eFluor 514 Calcium Sensor Dye is a membrane-permeable dye used for monitoring changes in intracellular free calcium concentrations in the cell using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. When it enters a cell, cellular esterases cleave the dye, resulting in a membrane-impermeable dye that fluoresces at ~520 nm. eFluor™ 514 Calcium Sensor Dyeis a commonly used dy…

DRAQ5, an anthraquinone dye with high affinity for double-stranded DNA, is a membrane-permeable dye that can label live or fixed/dead cells. The dye is used to distinguish nucleated and non-nucleated cells in flow cytometry, to report nuclear DNA content for ploidy and cell cycle analysis and as a nuclear counterstain nuclei in fluorescent microscopy.DRAQ5 can be excited by wavelengths from 488 to 647 nm. For microscopy/imagin…

The Cell Stimulation Cocktail (plus protein transport inhibitors) (500X) is a cocktail of phorbol 12-myristate 13-acetate (PMA), ionomycin, brefeldin A and monensin. PMA and ionomycin treatment can induce activation of many cell types to produce cytokines. Brefeldin A and Monensin result in the accumulation of secreted proteins in the endoplasmic reticulum and Golgi apparatus. The Cell Stimulation Cocktail (plus protein transp…

The Phytohemagglutinin-L (PHA-L) Solution (500X) is a ready-to-use solution of PHA-L in aqueous buffer. PHA is a mixture of lectins isolated from the red kidney bean, Phaseolus vulgaris. PHA-L is the fraction of PHA that exhibits strong leukoagglutinating and mitogenic activity, but very little erythroagglutinating activity. This reagent is intended for use in in vitro activation of human and mouse leukocytes.

The Concanavalin A (Con A) Solution (500X) is a ready-to-use solution of Con A in aqueous buffer. Con A, isolated from the Jack bean, Canavlia ensiformis, is a lectin that binds alpha-D-glucose and alpha-D-mannose moieties found in various glycoproteins, glycolipids, and sugars and is a potent leukocyte mitogen. This reagent is intended for use in in vitro activation of human and mouse leukocytes.

CellEvent Caspase-3/7 Green Ready Flow Reagent enables the flow cytometric detection of activated caspase-3 and caspase-7 in apoptotic cells. The product includes 2 reagents: the novel fluorogenic substrate CellEvent Caspase-3/7 Green Detection Reagent and SYTOX™ AADvanced™ Dead Cell Stain.FeaturesStable at room temperatureConvenient, ready-to-use format—no need to dilute, weigh, or pipetteExceptionally bright green fluorescen…

The Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay Kit provides a simplified, more robust assay for analyzing DNA replication in proliferating cells as compared to traditional BrdU methods. The Click-iT EdU Flow Cytometry Assay Kits rely on EdU (5-ethynyl-2´-deoxyuridine), a thymidine analog which is incorporated into DNA during active DNA synthesis, which is then detected using click chemistry, a copper catalyzed covalent…

The Click-iT EdU Alexa Fluor 488 Flow Cytometry Assay Kit provides a simplified, more robust assay for analyzing DNA replication in proliferating cells as compared to traditional BrdU methods. The Click-iT EdU Flow Cytometry Assay Kits rely on EdU (5-ethynyl-2´-deoxyuridine), a thymidine analog which is incorporated into DNA during active DNA synthesis, which is then detected using click chemistry, a copper catalyzed covalent…

The Click-iT Plus EdU Pacific Blue Flow Cytometry Assay Kit provides a simplified, more robust assay for analyzing DNA replication in proliferating cells as compared to traditional BrdU methods. The Click-iT EdU Flow Cytometry Assay Kits rely on EdU (5-ethynyl-2´-deoxyuridine), a thymidine analog which is incorporated into DNA during active DNA synthesis, which is then detected using click chemistry, a copper catalyzed covalen…

The Click-iT Plus EdU Alexa Fluor 350 Flow Cytometry Assay Kit provides a simplified, more robust assay for analyzing DNA replication in proliferating cells as compared to traditional BrdU methods. The Click-iT EdU Flow Cytometry Assay Kits rely on EdU (5-ethynyl-2´-deoxyuridine), a thymidine analog which is incorporated into DNA during active DNA synthesis, which is then detected using click chemistry, a copper catalyzed cova…

The Click-iT Plus EdU Alexa Fluor 594 Flow Cytometry Assay Kit provides a simplified, more robust assay for analyzing DNA replication in proliferating cells as compared to traditional BrdU methods. The Click-iT EdU Flow Cytometry Assay Kits rely on EdU (5-ethynyl-2´-deoxyuridine), a thymidine analog which is incorporated into DNA during active DNA synthesis, which is then detected using click chemistry, a copper catalyzed cova…

CellTrace CFSE Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry. Dye dilution analysis requires extremely bright fluorescent dyes, which is supplied by the Cell Trace CFSE dye. Using this dye, eight or more generations can be visualized. The reliable and homogeneous staining among cells in the population allows for visualization of dist…

CellTrace Violet Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry. Dye dilution analysis requires extremely bright fluorescent dyes, which is supplied by the Cell Trace Violet dye. Using this dye, ten or more generations can be visualized. The reliable and homogeneous staining among cells in the population allows for visualization of di…

CellTrace Far Red Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry. Dye dilution analysis requires extremely bright fluorescent dyes, which is supplied by the Cell Trace Far Red dye. Using this dye, six or more generations can be visualized. The reliable and homogeneous staining among cells in the population allows for visualization of…