Products, services, reviews and techniques used in the identification and validation of the disease-causing target genes and proteins.
Alexa 568-conjugated anticoagulant for the detection of phosphatidylserine on the outer leaflet of apoptotic cellsBenefits Detection flexibility: Analysis of results with flow cytometry and/or fluorescence microscopy. Alexa is a red dye, which can be used for double or triple staining with other fluorescent markers (e.g., fluorescein-labeled surface markers). Accuracy: Accurately distinguish apoptotic cells from necrotic…
Kit for the detection and quantification of apoptosis and differentiation from necrosis at the single-cell level, based on Annexin-V-labelingProduct Description Detection: Annexin-V-FLUOS can be directly detected in FACS analysis and immunochemistry without a secondary detection system. Sample material: Cell lines and freshly isolated cells. Specificity: Annexin-V-FLUOS binds in a Ca2+-dependent manner to negatively charged ph…
Roche Cell Death Detection ELISA - Photometric enzyme immunoassay for the quantitative in vitro determination of cytoplasmic histone-associated DNA fragments (mono- and oligonucleosomes) after induced cell deathBenefits of Roche Cell Death ELISA: High sensitivity: (5 x 102 cells/ml) Fast performance: (5 - 6 hours) No prelabeling of cells necessary Nonradioactive assay system No species restrictions Easy handling…
Kit for the detection and quantification of apoptotic cell death on a single-cell level by light microscopy in immunocyto- and immunohistochemistry Benefits Sensitive: The maximum intensity of labeling (cell staining) of apoptotic cells is higher than the nick translation method. Fast: The use of fluorescein-dUTP allows analysis of the samples directly after the TUNEL reaction, but before the addition of the secondary d…
Kit for the detection and quantification of apoptotic cell death on a single-cell level by flow cytometry and fluorescence microscopy, and for double labeling with fluorescein-labeled cell markers (TMR red)Benefits Convenient: No secondary detection system required. Accurate: Identification of apoptosis at a molecular level (DNA-strand breaks) and identification of cells at the very early stages of apoptosis. Flexible:…
Kit for the detection and quantification of apoptosis (programmed cell death) at the single-cell level, based on labeling of DNA strand breaks (TUNEL technology): Analysis by fluorescence microscopy or flow cytometry Benefits Sensitive: The direct labeling procedure using fluorescein-dUTP reduces background labeling. Fast: The use of fluorescein-dUTP allows analysis of the samples directly after the TUNEL reaction. Co…
Application The Cellular DNA Fragmentation ELISA may be applied to measure: Apoptosis by detection of BrdU-labeled DNA fragments in the cytoplasm of affected cells (= nonradioactive alternative to the [3H]-thymidine-based DNA-fragmentation assay). Cell-mediated cytotoxicity by detection of BrdU-labeled DNA fragments released from damaged target cells into the culture supernatant (= nonradioactive alternative to the [3H]-thymid…
Application The kit is used for the detection of BrdU incorporated into cellular DNA by immunocyto/histochemistry. Benefits Safe: No radioisotopes are used. Easy to perform: Follows a standard immunohistochemistry protocol. Sensitive: Denaturation of DNA with nucleases allows for highly sensitive detection of BrdU. Flexible: Allows double-labeling protocols.
Application The kit is used for the detection of BrdU incorporated into cellular DNA using immunofluorescence microscopy. It is used for the detection of DNA synthesis by either in vitro labeling of cells or organ cultures, or by in vivo labeling, in which frozen or paraffin-embedded tissue sections must be prepared prior to fixation. Benefits Safe: No radioisotopes are used. Easy to perform: Follows a standard immunof…
Application The Cell Proliferation ELISA, BrdU (colorimetric) belongs to the second, improved generation of kits for measuring DNA synthesis. It is designed as a precise, fast, and simple colorimetric alternative to quantitate cell proliferation based on the measurement of BrdU incorporation during DNA synthesis in replicating (cycling) cells. Thus, the Cell Proliferation ELISA can be used in many different in vitro cell syst…
