UHPLC and HPLC Columns Products & Reviews

400 bar; available in 2.1 - 4.6 mm id; 20 - 150 mm lengths. Manufactured by Agilent to rigorous QC specifications, the ZORBAX family encompasses 5 particle sizes and more than 32 phases, for reliable scalability and transferability from HPLC to UHPLC, from lab to lab and around the world.

Agilent AdvanceBio Peptide Mapping columns are designed to provide high-resolution peptide maps for protein identification and determination of post translation modifications. These columns provide the ability for you to quickly resolve and identify amino acid substitutions/modifications in a protein primary sequence.

ZORBAX RRHD 300-HILIC columns for glycopeptide analysis feature 1.8 µm particles and a 300Å pore size. These enable you to resolve peptide glycoforms.

LiChrospher silica columns are made with spherical, 'sil' type porous silica particles. LiChrospher Columns Features: Available in 5 µm particle size Reversed-phase available in RP-C18, RP-18 Encapped, RP-8, RP-Select B, CN Normal-phase available in CN, Diol, NH2, Si Applications—Pharmaceuticals, aromatics LiChrospher RP-18—Spherical silica for acidic, neutral and basic compounds with great batch-to-batch reproduci…

The ZORBAX NH2 can be utilized for normal-phase and weak anion-exchange compounds and can separate vitamins, carbohydrates and sugars in the reversed-phase mode. The NH2 consists of a propyl-amino silane phase bonded to ZORBAX SIL. For normal-phase chromatography, the ZORBAX product line offers a choice of bonded and non-bonded silica packings. ZORBAX NH2 Features: Amino-propyl silane phase bonded to ZORBAX SIL Used for…

The ZORBAX RX-SIL is made from very pure porous silica microspheres and is useful for very hydrophilic compounds with high organic mobile phases. With low acidity and metal content the ZORBAX Rx-SIL is ideal for normal-phase separation of polar compounds that exhibit poor peak symmetry on more acidic silica. The ZORBAX Rx-SIL is also stronger than other silica types and is less acidic than the ZORBAX-SIL. ZORBAX Rx-SIL Featu…

ResiPore columns are the ideal choice for the analysis of resins, and condensation polymers with complex molecular weight distributions that include oligomer content. By combining a low 3 µm particle size and high pore volume, high efficiency ResiPore columns offer maximum resolution of these intermediate molecular weight polymers. Features: Efficient separation of complex molecular weight distributions Reveals oligome…

Agilent AdvanceBio Glycan Mapping Columns are designed and manufactured to deliver FAST, high resolution, reproducible glycan identification using HILIC chromatography.

To successfully separate trityl-off, deprotected oligonucleotides, you need columns that have high resolving power and are robust enough to withstand challenging conditions.

The Affinity Removal LC Column - Human Albumin is designed to chromatographically remove the most interfering high-abundance protein (albumin) from human samples (e.g., plasma, serum, urine, or cerebrospinal fluid). They are compatible with most FPLC or HPLC pumps or instruments, including Agilent 1200/1100 instruments.

Agilent AdvanceBio SEC columns ensure easy, fast, and accurate quantitation for mAb aggregation and fragment analysis. AdvanceBio SEC offers two different particle sizes, 2.7 and 1.9 µm.

Agilent Bio SEC-3 HPLC columns are a breakthrough technology for size exclusion chromatography (SEC). They are packed with spherical, narrowly dispersed 3 µm silica particles coated with a proprietary hydrophilic layer.

Agilent Bio SEC-5 HPLC columns are packed with 5 µm silica particles coated with a proprietary, neutral, hydrophilic layer for maximum efficiency and stability. Our specially designed packing also promotes high pore volume, improving both peak capacity and resolution. 

Method validation is a critical step for biotherapeutic drug development. It ensures that the analytical method is suitable for its intended purpose, and that it meets regulatory requirements.

Agilent ZORBAX GF-250 and GF-450 size exclusion (gel filtration) columns are for size separations of proteins and other biomolecules. The separation range is 4,000-900,000 for globular proteins when using GF-250 and GF-450 columns in series.

Agilent AdvanceBio MS Spent Media columns are HILIC columns that deliver fast, sensitive, and reproducible separations of underivatized amino acids and other polar metabolites found in bioprocessor cell culture media for mass spectrometric detection.

Agilent Bio Ion-Exchange HPLC columns are packed with polymeric, nonporous, ion-exchange particles. Bio Ion-Exchange Columns are designed for high resolution, high recovery and highly efficient separations of peptides, oligonucleotides and proteins.

Thorough characterization of monoclonal antibodies includes the identification and monitoring of acidic and basic isoforms. The Agilent Bio MAb HPLC columns feature a unique resin specifically designed for high-resolution charge-based separations of monoclonal antibodies.

PL-SAX for Biomolecules is ideal for the anion-exchange HPLC separations of proteins and deprotected synthetic oligonucleotides under denaturing conditions.

PL-SCX is a macroporous PS/DVB matrix with a very hydrophilic coating and strong cation-exchange functionality. 

ZORBAX Ion Exchange columns are available as both Strong Anion-Exchange (SAX) and Strong Cation-Exchange (300SCX) columns. 

AdvanceBio Desalting-RP cartridge-style columns can be used on any LC system for on-line desalting of collected fractions. They can also be used on an Agilent 1290 Infinity II 2D-LC system as the second dimension to desalt after the first-dimension separation.

Agilent AdvanceBio RP-mAb columns are designed to optimize the performance of intact and reduced mAb analysis when analyzing monoclonal antibodies for biopharma discovery, development, and QA/QC applications

PLRP-S for Biomolecules products are available in a range of pore sizes and particle sizes, all with identical chemistry and fundamental adsorptive characteristics. The particles are inherently hydrophobic so there is no bonded phase, alkyl ligand required for reversed-phase separations.

Agilent ZORBAX 300Å StableBond columns are an ideal choice for the reproducible separations of proteins and peptides.