UHPLC and HPLC Columns Products & Reviews

YMC-Triart: Robust and reproducible (U)HPLC columns with a wide range of stability towards pH (1-12), temperature and 100% aqueous eluents.

Coated and immobilized CHIRAL ART polysaccharide columns by YMC offer wide selectivity range, high stability and attractive pricing.

Capillary columns available with every fully porous RP/NP/HILIC phase from YMC for high sensitivity analyses. Compatible with nearly all Nano-/MicroLC/MS systems due to two different connection sizes (1/16" and 1/32").  

Highly robust (U)HPLC columns for BioLC which are stable towards pH and temperature. Different pore sizes are available for the analysis of peptides, proteins, antibodies and oligonucleotides. 

200A pore size;  available in 2.0, 3.0, 3.9, 4.0, 4.6, 10 , 21.2 and 50  mm ids, and lengths from 50 - 250 mm. Pursuit columns are ideal for pharmaceutical applications using LC/MS, from analyzing lead compounds and biological samples in drug discovery, to implementing dependable analysis of raw materials and approved drugs in the QC process.  Build on the large 200A pore size silica, high liquid density delivers up to 40% fas…

200A pore size;  available in 2.0, 4.6, 10and 21.2  mm ids, and lengths from 50 - 250 mm. Pursuit columns are ideal for pharmaceutical applications using LC/MS, from analyzing lead compounds and biological samples in drug discovery, to implementing dependable analysis of raw materials and approved drugs in the QC process.  Build on the large 200A pore size silica, high liquid density delivers up to 40% faster separations witho…

600 bar; superficially porous, 2.7 µm particle6; available in 2.1 - 4.6 mm id;  30 - 150 mm lengths. ZORBAX Eclipse Plus columns feature improved silica, special bonding and optimized endcapping that combine to deliver exceptional peak shapes for a wide range of analytes.   Agilent's Poroshell 120 columns have a solid 1.7 µm core and a porous .5 µm outer layer, for a total 2.7 µm particle that delivers high resolution and high…

600 bar; superficially porous, 2.7 µm particle6; available in 2.1 - 4.6 mm id;  30 - 150 mm lengths. ZORBAX Eclipse Plus columns feature improved silica, special bonding and optimized endcapping that combine to deliver exceptional peak shapes for a wide range of analytes.  Agilent's Poroshell 120 columns have a solid 1.7 µm core and a porous .5 µm outer layer, for a total 2.7 µm particle that delivers high resolution and high…

400 bar; available in 2.1 - 4.6 mm id; 30, 50, 75, 100, 150 mm lengths. Manufactured by Agilent to rigorous QC specifications, the ZORBAX family encompasses 5 particle sizes and more than 32 phases, for reliable scalability and transferability from HPLC to UHPLC, from lab to lab and around the world.

Extend-C18 columns incorporate a unique patented bidentate silane, combined with a double-endcapping process that protects the silica from dissolution at high pH - up to pH 11.5. 

Eclipse Plus columns provide the ultimate in performance for silica-based columns. Peak shape for the Eclipse Plus is excellent for the most challenging basic compounds, improving efficiency and resolution with these sample types. These results are achieved by improvements in the silica manufacturing and bonding technology, which is completely controlled by Agilent. 

Agilent Bio-Monolith Protein A and Protein G LC columns are designed for analytical separation of all IgG subclasses (human and mouse). They deliver fast and accurate quantitation of mAb titer and purification of small amounts of mAb for further characterization.

In order to more easily isolate and identify proteins in human serum, the Agilent Human 14 Multiple Affinity Removal Column is designed to chromatographically remove fourteen interfering high-abundant proteins from human plasma samples.

Agilent Poroshell columns are ideal for fast separations of proteins and peptides because the unique particle allows for fast flow rates to be used while maintaining sharp, efficient peaks. 

Agilent ZORBAX Eclipse XDB columns – C18, C8, Phenyl and CN – provide four bonded phase choices for method development optimization. These columns provide good peak shape over a wide pH range (2–9) for additional method development flexibility with one family of columns. Eclipse XDB columns can be used for method development at low pH (2–3) and the same column can be used for method development in the mid pH (6–8) region.

Poroshell 120 columns provide rugged, high resolution separations within the pressure range of any mainstream LC system, making the benefits of sub-2 µm performance available with all existing LCs.Poroshell columns can achieve high resolution and high speed separations on current instruments, and higher resolution and speeds on new high-pressure LC and LC/MS systems. Stable to 600 bar, Poroshell 120 are great for fast UHPLC an…

Protein/Peptide Columns

Most chromatographers have experienced problems retaining and separating polar compounds using conventional reversed-phase chromatography.  These difficult-to-analyze compounds either pass through the column unretained or, if retained at all, co-elute at the beginning of the chromatogram.  Waters AtlantisTM columns are designed for these types of challenging separations and are available in two chemistries: dC18 and HILIC sili…

Unleash the power of your laboratory's instrumentation. CORTECS® 1.6 µm and 2.7 µm solid-core columns deliver new levels of performance. CORTECS Columns are available in both reversed-phase and HILIC options, giving you the flexibility and power to develop methods for challenging separations even faster. With different particle characteristics and innovative charged surface modifications, CORTECS Columns are suitable for use…

ACQUITY UPC2 Column Technology for Chiral and Achiral Separations. ACQUITY UPC2 Trefoil™ 2.5 µm Columns are uniquely designed for the ACQUITY UPC2 System. Selectivity and speed for chiral separations Reduce method development time Based on modified polysaccharide-based stationary phases for broad-spectrum chiral selectivity. ACQUITY UPC2 Torus™ Columns simplify the method development process with four completely new…

With Raptor™ LC columns, Restek chemists became the first to combine the speed of superficially porous particles (also known as SPP or “core-shell” particles) with the resolution of highly selective USLC® technology. This new breed of chromatographic column allows you to more easily achieve peak separation and faster analysis times without expensive UHPLC instrumentation. SPP core-shell columns commonly employ traditional phe…

Separate a wide variety of polar analytes with a novel hybrid stationary phase

Inertsil ODS columns are general-purpose reversed phase C18 columns, available in 5-µm and 10-µm particle sizes. It was the first of the ODS columns from GL Sciences, introduced back in 1986. We currently recommend InertSustain C18 or Inertsil ODS-4 columns for all new method developments.

“Allround“ SEC-columns for initial analysis of unknown samples

TSKgel GPC columns for organic-soluble polymers are packed with spherical particles composed of polystyrene crosslinked with divinylbenzene (PS-DVB)