Western blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
The liquid BCIP/NBT is the most commonly used substrate for colorimetric determination of Alkaline Phosphatase (AP) activity in membrane tests. We offer an aqueous buffered solution containing NBT (NitroBlue Tetrazolium) and BCIP (Bromo-Chloro-Indolyl Phosphate) forming a blue/purple precipitate at the positive sites. The colour develops when AP catalyses the dephosphorylation of BCIP. The intense blue/purple precipitate is…
TMB (3,3´,5,5´- tetramethylbenzidine) is a substrate for horseradish peroxidase (HRP). Our mono-component, liquid TMB solutions combine TMB, substrate buffer and hydrogen peroxide for a safe, ready-to-use, one step, chromogen system. The products do not contain harmful solvents. Recommended for ELISA procedures, liquid TMB yields a soluble blue end product read at 655 nm. The TMB reaction may be stopped with 0.2 M H2SO4 resu…
This new type of TMB with a red indicator combines all the important qualities of TMB ONE, eliminating possible mistakes during pipetting. Further the red colour may be useful in automatic analyzers giving the warning "Reagent low" when it is time for filling up the substrate.The red indicator disappears after addition of the stopping solutionand will neither affect the sensitivity nor the stability of the substrate. Stability…
The TMB PLUS has a higher activity level, resulting in a higher sensitivity and a reduced incubation time than found using the TMB ONE. TMB is a substrate for HRP. The mono-component, liquid TMB solution combines TMB, substrate buffer and hydrogen peroxide for a safe, ready-to-use, one step, chromogen system.The product does not contain harmful solvents. Recommended for ELISA procedures, liquid TMB yields a soluble blue end pr…
This new type of TMB with a red indicator combines all the important qualities of TMB PLUS, eliminating possible mistakes during pipetting.Further the red colour may be useful in automatic analyzers giving the warning "Reagent low" when it is time for filling up the substrate. The red indicator disappears after addition of the stopping solution and will neither affect the sensitivity nor the stability of the substrate. Stabili…
TMB is a substrate for HRP. Our mono-component, liquid TMB solution combine TMB, substrate buffer and hydrogen peroxide for a safe, ready-to-use, one step, chromogen system.The products do not contain harmful solvents. The TMB X-tra is a unique TMB substrate with an excellent resistancy and the highest sensitivity.
This TMB X-tra with red indicator combines all the important qualities of TMB X-tra eliminating possible mistakes during pipetting.Further the red colour may be useful in automatic analyzers giving the warning “Reagent low” when it is time for filling up the substrate. The red indicator disappears after addition of the stopping solution and will neither affect the sensitivity nor the stability of the substrate.
The TMB Blotting are ready-to-use substrates containing 3,3´, 5, 5´ tetramethylbenzidine and hydrogen peroxide.These substrates are intended for use in blotting procedures with peroxidase labelled antibodies. The positive reaction gives a stable blue colour, and the products give a good sensitivity and a low background staining. Stability life time is 2 ½ years at 4°C.
The TMB Blotting PLUS are ready-to-use substrates containing 3,3´, 5, 5´ tetramethylbenzidine and hydrogen peroxide.These substrates are intended for use in blotting procedures with peroxidase labelled antibodies. The positive reaction gives a stable blue colour, and the products give a good sensitivity and a low background staining. Stability life time is 2 ½ years at 4°C.
TMB-D is a liquid substrate for use in colourimetric blotting assays based upon HRP labelled antibodies. The TMB-D Blotting is a distinctive blotting substrate with superior background characteristics. Stability life time is 2 ½ years at 4°C.
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New pNPP ONE Substrate – The pNPP (p-NitroPhenyl Phosphate) liquid, ready-to-use substrate is a highly active and stable formulation utilized for measuring AP- (Alkaline Phosphatase) probe activity. The solution is a solvent free buffer solution containing stabilized pNPP using sodium azide as preservative. In kinetic assays p-nitrophenol appears yellow and is measured at 405 /650 nm. If stopped reactions are preferred, add…
Say goodbye to secondary antibodies – Go direct using the World’s Easiest DIY Antibody Labeling Kit - Lightning-Link™! • Eliminates need for secondary antibodies • Reduces the number of wash steps • Ideal for multi-protein analysis – with over 40 available labels • Very easy to use • 100% antibody recovery Lightning-Link™ kits The DIY Lightning-Link™ kit allows you to label an antibody with minimal hands-on time - less than 30…
LifeXtend™ conjugate stabilizer/diluent is a proprietary multi-component reagent system that stabilizes antibody-HRP conjugates both at 4°C and at ambient temperature. By using LifeXtend™ HRP stabilizer/diluent in antibody conjugation the HRP conjugate can be stabilized during use and stored as pre-diluted, ready-to-use reagents thus eliminating waste and improving consistency from experiment to experiment. LifeXtend™ shows…
For simple, cost-effective protein detection, use Millipore's Western blotting reagents to achieve the highest sensitivity with the least background. Bløk™ Noise Cancelling Reagents Bløk reagents minimize background in Western blots, increasing sensitivity for superior detection of even low-abundance proteins. Bløk reagents are protein-free, which reduces non-specific binding, especially when you are using phosphoantibodies o…
Say goodbye to secondary antibodies – Go direct using the World’s Easiest DIY Antibody Labeling Kit - Lightning-Link™! • Eliminates need for secondary antibodies • Reduces the number of wash steps • Ideal for multi-protein analysis – with over 40 available labels • Very easy to use • 100% antibody recovery Lightning-Link™ kits The DIY Lightning-Link™ kit allows you to label an antibody with minimal hands-on time - less than 30…
Say goodbye to secondary antibodies – Go direct using the World’s Easiest DIY Antibody Labeling Kit - Lightning-Link™! • Eliminates need for secondary antibodies • Reduces the number of wash steps • Ideal for multi-protein analysis – with over 40 available labels • Very easy to use • 100% antibody recovery Lightning-Link™ kits The DIY Lightning-Link™ kit allows you to label an antibody with minimal hands-on time - less than 30…
Say goodbye to secondary antibodies – Go direct using the World’s Easiest DIY Antibody Labeling Kit - Lightning-Link™! • Eliminates need for secondary antibodies • Reduces the number of wash steps • Ideal for multi-protein analysis – with over 40 available labels • Very easy to use • 100% antibody recovery Lightning-Link™ kits The DIY Lightning-Link™ kit allows you to label an antibody with minimal hands-on time - less than 30…
Say goodbye to secondary antibodies – Go direct using the World’s Easiest DIY Antibody Labeling Kit - Lightning-Link™! • Eliminates need for secondary antibodies • Reduces the number of wash steps • Ideal for multi-protein analysis – with over 40 available labels • Very easy to use • 100% antibody recovery Lightning-Link™ kits The DIY Lightning-Link™ kit allows you to label an antibody with minimal hands-on time - less than 30…
Say goodbye to secondary antibodies – Go direct using the World’s Easiest DIY Antibody Labeling Kit - Lightning-Link™! • Eliminates need for secondary antibodies • Reduces the number of wash steps • Ideal for multi-protein analysis – with over 40 available labels • Very easy to use • 100% antibody recovery Lightning-Link™ kits The DIY Lightning-Link™ kit allows you to label an antibody with minimal hands-on time - less than 30…
Say goodbye to secondary antibodies – Go direct using the World’s Easiest DIY Antibody Labeling Kit - Lightning-Link™! • Eliminates need for secondary antibodies • Reduces the number of wash steps • Ideal for multi-protein analysis – with over 40 available labels • Very easy to use • 100% antibody recovery Lightning-Link™ kits The DIY Lightning-Link™ kit allows you to label an antibody with minimal hands-on time - less than 30…
Say goodbye to secondary antibodies – Go direct using the World’s Easiest DIY Antibody Labeling Kit - Lightning-Link™! • Eliminates need for secondary antibodies • Reduces the number of wash steps • Ideal for multi-protein analysis – with over 40 available labels • Very easy to use • 100% antibody recovery Lightning-Link™ kits The DIY Lightning-Link™ kit allows you to label an antibody with minimal hands-on time - less than 30…
Say goodbye to secondary antibodies – Go direct using the World’s Easiest DIY Antibody Labeling Kit - Lightning-Link™! • Eliminates need for secondary antibodies • Reduces the number of wash steps • Ideal for multi-protein analysis – with over 40 available labels • Very easy to use • 100% antibody recovery Lightning-Link™ kits The DIY Lightning-Link™ kit allows you to label an antibody with minimal hands-on time - less than 30…
Say goodbye to secondary antibodies – Go direct using the World’s Easiest DIY Antibody Labeling Kit - Lightning-Link™! • Eliminates need for secondary antibodies • Reduces the number of wash steps • Ideal for multi-protein analysis – with over 40 available labels • Very easy to use • 100% antibody recovery Lightning-Link™ kits The DIY Lightning-Link™ kit allows you to label an antibody with minimal hands-on time - less than 30…
Say goodbye to secondary antibodies – Go direct using the World’s Easiest DIY Antibody Labeling Kit - Lightning-Link™! • Eliminates need for secondary antibodies • Reduces the number of wash steps • Ideal for multi-protein analysis – with over 40 available labels • Very easy to use • 100% antibody recovery Lightning-Link™ kits The DIY Lightning-Link™ kit allows you to label an antibody with minimal hands-on time - less than 30…
