Protein Purification Products & Reviews

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Protein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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PL-SAX for Prep to Process

Agilent Technologies

PL-SAX strong anion-exchange materials are extremely hydrophilic and are designed for purification of biomolecules. The materials are totally polymeric and are chemically and thermally stable over a full range of HPLC conditions.

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PL-SCX for Prep to Process

Agilent Technologies

PL-SCX strong cation-exchange materials are extremely hydrophilic and are designed for purification of biomolecules. The materials are totally polymeric and are chemically and thermally stable over a full range of HPLC conditions.

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Axygen® AxyPrep MAG PCR Clean-Up Kit - 500 mL

Corning Life Sciences

The AxyPrep Mag PCR Clean-Up Kit is a unique paramagnetic bead-based post PCR clean-up kit designed for efficient removal of salts, primers, primer-dimers, dNTPs, and enzymes. Highly purified DNA is eluted with an optimized buffer so that it can be used directly for downstream applications, such as library construction, next generation sequencing (NGS), Sanger sequencing, cloning, restriction digestions, adapter ligation, and…

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cOmplete Lysis-Y

Reagent kit that enables rapid lysis of yeast cells for highly efficient protein extraction and simultaneous protection of extracted proteins against a multitude of proteases Application Use Complete Lysis-Y to rapidly lyse cells of various yeast strains (e.g., Saccharomyces cerevisiae, Schizosaccharomyces pombe, Pichia pastoris) in only 20 minutes at room temperature and simultaneously inhibit protease activity in the cell ly…

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cOmplete Lysis-B (2x), EDTA-free

Reagent kit that enables rapid lysis of bacteria for highly efficient protein extraction and concurrent protection of extracted proteins against a multitude of proteases. Suitable for downstream purification using IMAC. Application Use Complete Lysis-B (2x), EDTA-free to rapidly lyse bacteria in only 10 minutes and simultaneously inhibit protease activity in the cell lysate. The kit enables highly efficient protein extraction…

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cOmplete Lysis-B (2x)

Reagent kit that enables rapid lysis of bacteria for highly efficient protein extraction and concurrent protection of extracted proteins against a multitude of proteases Application Use Complete Lysis-B (2x) to rapidly lyse bacteria in only 10 minutes and simultaneously inhibit protease activity in the cell lysate. The kit enables highly efficient protein extraction and concurrent inhibition of a multitude of proteases, includ…

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cOmplete Lysis-M, EDTA-free

Reagent kit that enables rapid lysis of mammalian cells for highly efficient protein extraction and simultaneous protection of extracted proteins against a multitude of proteases. Suitable for downstream purification using IMAC. Application Use Complete Lysis-M, EDTA-free to rapidly lyse mammalian cells in only 5 minutes and simultaneously inhibit protease activity in the cell lysate. The kit enables highly efficient protein e…

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cOmplete Lysis-M

Reagent kit that enables rapid lysis of mammalian cells for highly efficient protein extraction and simultaneous protection of extracted proteins against a multitude of proteases Application Use Complete Lysis-M to rapidly lyse mammalian cells in only 5 minutes and simultaneously inhibit protease activity in the cell lysate. The kit enables highly efficient protein extraction and concurrent inhibition of a multitude of proteas…

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cOmplete Lysis-Y, EDTA-free

Reagent kit that enables rapid lysis of yeast cells for highly efficient protein extraction and simultaneous protection of extracted proteins against a multitude of proteases. Suitable for downstream purification using IMAC. Application Use Complete Lysis-Y, EDTA-free for the rapid cell lysis of various yeast strains (e.g., Saccharomyces cerevisiae, Schizosaccharomyces pombe, Pichia pastoris) in only 20 minutes at room tempera…

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Streptavidin

The Steptavidin is produced by fermentation of S. avidinii in a minimal medium. The protein has a structure with four identical subunits, each containing a binding site for biotin. It is stable over a wide range of pH and decomposes only in the presence of SDS at temperatures above 60 °C. Steptavidin conjugated with an enzyme, i.e. Horseradish Peroxidase or Alkaline Phosphatase or a fluorochrome may be used to visualize biot…

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Empore™ Accessory Vacuum Manifold for 96 Well Plates

3M Bioanalytical

The Empore™ High Performance Extraction Disk Plate differs in design from plates offered by other manufacturers and requires a specific vacuum manifold.  Manifolds supplied by different plate manufacturers are specific to the design of their plates and cannot generally be used with plates from other manufacturers.  In some cases, the use of spacers to raise/lower the height of a collection device inside the manifold may overco…

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Bio-Monolith

Agilent Technologies

Agilent Bio-Monolith HPLC columns provide high resolution and rapid separations of antibodies (IgG, IgM), plasmid DNA, viruses, phages and other macro biomolecules. The product family offers strong cation exchange, strong and weak anion exchange, and Protein A phases.

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AbSelect™ Antibody Purification System

Innova Biosciences Ltd.

Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions with enzymes or fluorophores. The AbSelect™ Purification Kit quickly removes these contaminants of antibody labelling. It can also be used to purify antibodies from crude samples such as ascites fluid or immune serum. The antibody to be purified or cleaned up ideally is in a volume of 100ul to 0.5m…

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Strep-tag® Starter Kit

IBA BioTAGnology

The Strep-tag® / Strep-Tactin® purification system allows the isolation of above 95% pure and functional proteins after one chromatographical step. The Strep-tag system is based on the highly selective and easily controllable interaction between the Strep-tag II peptide and the specially engineered streptavidin called Strep-Tactin. The binding affinity of Strep-tag II to Strep-Tactin is nearly 100 times higher than to Stre…

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Strep-Tactin® Superflow®

IBA BioTAGnology

The recently launched Strep-Tactin® Superflow® high capacity resin offers improved binding capacity but identical high protein purity compared to standard Strep-Tactin® Superflow®. Thus, it is the perfect solution for Strep-tag® protein purification. Both Strep-Tactin® Superflow® resins • combine outstanding flow characteristics with superior mechanical stability • are suitable for increased flow rates and useful for purificat…

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