PCR and Thermal Cycling Products & Reviews

200 x 20 µl reactions, includes 20x  Residual DNA Quantification Assay and ddPCR Supermix for Residual DNA Quantification. For use with the QX100™, QX200™, or AutoDG™ Droplet Digital™ PCR Systems

200 x 20 µl reactions, includes 20x CHO Residual DNA Quantification Assay and ddPCR Supermix for Residual DNA Quantification. For use with the QX100™, QX200™, or QX200™ AutoDG™ Droplet Digital™ PCR Systems  

The QXDx AutoDG ddPCR System is Bio-Rad's Droplet Digital PCR system that is FDA-cleared for use with QXDx BCR-ABL %IS Kit monitoring in the clinical lab. The same system, which includes the QXDx Automated Droplet Generator and QXDx Droplet Reader, provides precise and absolute quantification of target DNA and RNA molecules in a wide variety of research applications.

The probe-based Vericheck ddPCR Mycoplasma detection Kit brings confidence in testing for mycoplasma contamination. The specificity and reproducibility of this kit allows for a precise measurement of 112 mycoplasma species using droplet digital PCR.

User-friendly automated benchtop capping and decapping instrument to streamline labor-intensive workflows. 

Bio-Rad’s ddPCR Cell and Gene Therapy (CGT) Assays are custom ddPCR-optimized assays generated by the Cell and Gene Therapy Design Engine for your target Gene of Interest. These assays offer the ease and accuracy of ddPCR technology and come in multiple fluorophores to enable multiplexed Cell and Gene Therapy applications, including Viral Titer and Vector Copy Number measurements. Use the ddPCR CGT Assays individually or multi…

The Vericheck ddPCR HEK293 Residual DNA Quantification Kit, with a novel ddPCR assay, has been designed to quantify residual HEK293 DNA in biotherapeutics with high specificity and reproducibility, without the need for reference curves.

The PREvalence ddPCR™  SARS-CoV-2 Wastewater Quantification Kit provides reproducible, quantitative results in wastewater testing for SARS-CoV-2, the virus caused by COVID-19.

Lentiviruses are commonly used as viral vectors in cell and gene therapy production, delivering and steadily integrating desired genes into the genome of the host cell. However, they may also present health hazards to humans due to integration-mediated transformation and the creation of a RCL that can infect nontarget cells. While the current regulatory guidelines recommend a cell culture–based method for RCL testing, it is cu…

Adeno-associated virus (AAV) is commonly used as a viral vector in cell and gene therapy production, but there is a risk of it becoming a replication competent virus (RCV) during the production process, especially in the presence of a helper virus, which can raise safety concerns. While the guidelines recommend a cell culture–based method for RCAAV testing, it is cumbersome and takes 30–45 days from sample to results. Verichec…

The Universal Human miRNA Reference RNA is an ideal reference control for miRNA microarray or miRNA-targeted QRTPCR experiments. The Universal Human miRNA Reference RNA may also be used as an optimization or standardization reagent for these or other applications aimed at human miRNA analysis.

High-quality total RNA for human, mouse, and rat microarray gene-expression profiling that acts as a consistent control for standard data set comparisons.

The Agilent DNA Fish ID solution uses advanced software to achieve more accurate results – quickly and easily. Key to the process is the Agilent 2100 Bioanalyzer and RFLP Decoder Software performs RFLP pattern matching, which eliminates the need for manual calling

Allows in vivo excision of cloned fragments in the phagemid vector Screening with both antibodies and nucleic acid probes Each premade library comes with necessary strains and helper phage  

Our Mycoplasma Plus PCR Primer Set detects infection by all forms of Mycoplasma with a robust single-band PCR product (triplet for Acholeplasma). This kit also includes controls to verify PCR amplification and Mycoplasma genomic DNA. The primer design permits higher annealing temperatures, reducing spurious bands from PCR contaminants.

The MycoSensor PCR & QPCR Assay Kits detect Mycoplasma infection of cell cultures by real-time quantitative PCR (qPCR) or PCR in less than two hours.

Mx Pro QPCR software with FDA guideline 21 CFR Part 11 compatible features. Four key elements have been added to create the ET software version; secure application login, database file management, electronic audit trail, and report generation.  

AccuScript High-Fidelity Reverse Transcriptase (RT) delivers 3.7x more accuracy than other commercially available reverse transcriptases and 6.6x more accuracy than AMV RT. The AccuScript PfuUltra II RT-PCR kit provides a complete system in applications that demand high-fidelity, specificity, and throughput.

AccuScript High-Fidelity Reverse Transcriptase (RT) delivers 3.7x more accuracy than other commercially available reverse transcriptases and 6.6x more accuracy than AMV RT. The AccuScript PfuUltra II RT-PCR kit provides a complete system in applications that demand high-fidelity, specificity, and throughput.

AffinityScript Multiple Temperature Reverse Transcriptase (RT) produces high cDNA yields over the broadest temperature range with high affinity to primer/template complexes. It delivers high cDNA yield when amplifying low RNA input amounts and resolving RNA secondary structure using temperatures up to 55°C.

Alien Reference RNA QRT-PCR Detection Kit for Monitoring the Overall Performance of QRT-PCR Assays.

Alien Reference RNA QRT-PCR Detection Kit for Monitoring the Overall Performance of QRT-PCR Assays.

Brilliant II QPCR and QRT-PCR reagents were developed for improved sensitivity of detection, ensuring reproducible quantification even at low target concentrations. For faster experiments with earlier Ct's and greater reproducibility, Agilent recommends Brilliant III Ultra-Fast master mixes.

Brilliant II QPCR and QRT-PCR reagents were developed for improved sensitivity of detection, ensuring reproducible quantification even at low target concentrations. For faster experiments with earlier Ct's and greater reproducibility, Agilent recommends Brilliant III Ultra-Fast master mixes.

Easy-A high-fidelity cloning enzymes combines the higher accuracy of a proofreading enzyme with the high cloning efficiency of Taq. PCR products amplified with the Easy-A can be cloned directly, without performing additional steps typically required when amplifying with proofreading polymerases.