UHPLC and HPLC Products & Reviews

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High performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Peristaltic Pump

Agilent Technologies

Flexible and precise sample transfer   The flexible, bi-directional Peristaltic Pump is specifically designed to be used in dissolution sample transfer, even allowing the use of surfactants without excessive foaming. It has a 16-channel maximum capacity for sample transfer with the Agilent 8000 Dissolution Sampling System. The peristaltic pumping system incorporates an 8-roller design for smooth flow.

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UV Dissolution Software

Agilent Technologies

Customized automation for best dissolution results   The new Agilent WinUV Dissolution Software generates accurate and robust data. The software supports multiple hardware configurations for sampling, filtering, and archiving your samples. Designed to run on Microsoft® Windows® XP, it provides a 21 CFR Part 11 compliant environment for your laboratory data and operations.

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Polaris C8-A HPLC Columns

Agilent Technologies

Agilent Polaris HPLC columns offer many unique phase chemistries designed to retain both polar and nonpolar analytes, reliably and with excellent peak shape. The various Polaris phases are based on modified C8 and C18 bonded phases, each offering different selectivities. The combination of high phase density bonding, ultrapure silica, and silanol shielding provides outstanding chromatography with highly polar samples.

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Polaris C18-Ether HPLC Columns

Agilent Technologies

Agilent Polaris HPLC columns offer many unique phase chemistries designed to retain both polar and nonpolar analytes, reliably and with excellent peak shape. The various Polaris phases are based on modified C8 and C18 bonded phases, each offering different selectivities. The combination of high phase density bonding, ultrapure silica, and silanol shielding provides outstanding chromatography with highly polar samples.

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Polaris C8-Ether HPLC Columns

Agilent Technologies

Agilent Polaris HPLC columns offer many unique phase chemistries designed to retain both polar and nonpolar analytes, reliably and with excellent peak shape. The various Polaris phases are based on modified C8 and C18 bonded phases, each offering different selectivities. The combination of high phase density bonding, ultrapure silica, and silanol shielding provides outstanding chromatography with highly polar samples.

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Polaris NH2 HPLC Columns

Agilent Technologies

Agilent Polaris HPLC columns offer many unique phase chemistries designed to retain both polar and nonpolar analytes, reliably and with excellent peak shape. The various Polaris phases are based on modified C8 and C18 bonded phases, each offering different selectivities. The combination of high phase density bonding, ultrapure silica, and silanol shielding provides outstanding chromatography with highly polar samples.

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Polaris Si-A HPLC Columns

Agilent Technologies

Agilent Polaris HPLC columns offer many unique phase chemistries designed to retain both polar and nonpolar analytes, reliably and with excellent peak shape. The various Polaris phases are based on modified C8 and C18 bonded phases, each offering different selectivities. The combination of high phase density bonding, ultrapure silica, and silanol shielding provides outstanding chromatography with highly polar samples.

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Pursuit Diphenyl HPLC Columns

Agilent Technologies

The Pursuit family of HPLC columns offer strong performance in a variety of common phase chemistries. Columns are available in many dimensions, particle sizes, and pore sizes to fit various needs. Media is scalable from analytical all the way up to preparative dimensions.

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Stable Bond SB-C18

Agilent Technologies

ZORBAX 80Å StableBond columns are made using bulky, patented, unique silanes that sterically protect the siloxane bond. Acid labile endcapping reagents are not used. The result is vastly improved column life and extraordinary chemical and temperature stability in the pH 1–6 range for a wide variety of phases (SB-Aq, SB-C3, SB-CN, SB-Phenyl, SB-C8, and SB-C18).

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Eclipse XDB-CN (USP L10)

Agilent Technologies

Agilent ZORBAX Eclipse XDB columns – C18, C8, Phenyl and CN – provide four bonded phase choices for method development optimization. These columns provide good peak shape over a wide pH range (2–9) for additional method development flexibility with one family of columns. 

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ZORBAX StableBond 80Å SB-C8 (USP L7)

Agilent Technologies

ZORBAX 80Å StableBond columns are made using bulky, patented, unique silanes that sterically protect the siloxane bond. Acid labile endcapping reagents are not used. The result is vastly improved column life and extraordinary chemical and temperature stability in the pH 1–6 range for a wide variety of phases (SB-Aq, SB-C3, SB-CN, SB-Phenyl, SB-C8, and SB-C18). Excellent peak shape is expected for many basic compounds because…

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ZORBAX StableBond 80Å SB-CN (USP L10)

Agilent Technologies

ZORBAX 80Å StableBond columns are made using bulky, patented, unique silanes that sterically protect the siloxane bond. Acid labile endcapping reagents are not used. The result is vastly improved column life and extraordinary chemical and temperature stability in the pH 1–6 range for a wide variety of phases (SB-Aq, SB-C3, SB-CN, SB-Phenyl, SB-C8, and SB-C18). Excellent peak shape is expected for many basic compounds because…

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ZORBAX StableBond 80Å SB-Phenyl (USP L11)

Agilent Technologies

ZORBAX 80Å StableBond columns are made using bulky, patented, unique silanes that sterically protect the siloxane bond. Acid labile endcapping reagents are not used. The result is vastly improved column life and extraordinary chemical and temperature stability in the pH 1–6 range for a wide variety of phases (SB-Aq, SB-C3, SB-CN, SB-Phenyl, SB-C8, and SB-C18). Excellent peak shape is expected for many basic compounds because…

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ZORBAX StableBond 80Å SB-Aq

Agilent Technologies

ZORBAX 80Å StableBond columns are made using bulky, patented, unique silanes that sterically protect the siloxane bond. Acid labile endcapping reagents are not used. The result is vastly improved column life and extraordinary chemical and temperature stability in the pH 1–6 range for a wide variety of phases (SB-Aq, SB-C3, SB-CN, SB-Phenyl, SB-C8, and SB-C18). Excellent peak shape is expected for many basic compounds because…

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