UHPLC and HPLC Products & Reviews

Hydrophobic Interaction Chromatography Columns for the Separation of Large BiomoleculesHydrophobic Interaction Chromatography separates biomolecules in a decreasing salt gradient, based on differences in surface hydrophobicity. This method preserves the biological activity of proteins. The HIC separation mechanism is complementary to those of ion-exchange and gel filtration chromatography.The ProPac® HIC-10 column is a high-re…

ProSwift™ ConA-1S Affinity Column for Glycan, Glycopeptide and Glycoprotein Enrichment and Purification The ProSwift ConA-1S affinity monolith column is unsurpassed for fast, highly efficient enrichment and purification of Concanavalin A (Con A) binding glycans, glycopeptides, and glycoproteins from complex samples.The HPLC- compatible ProSwift ConA-1S affinity column maintains the specificity and selectivi…

Monolith Reversed-Phase Columns for High-Resolution Protein Separations at High Flow Rates ProSwift® reversed-phase monolith columns uniquely provide the advantages of high resolution at exceptionally high flow rates for protein separations. They deliver the outstanding resolving power of nonporous analytical media combined with faster separations than any bead based columns available. ProSwift polymer monolith columns achieve…

Monolith Ion-Exchange Columns for Superior High-Resolution Analytical and Preparative Protein Separations The ProSwift SCX monolith column line has been expanded to include a new 1 x 50 mm column. As with all ProSwift monoliths, the fast mass transfer allows for fast protein separations. ProSwift monoliths offer excellent separations using fast gradients at low and high flow rates, which improve productivity.ProSwift Monolith…

The Acclaim™ C30 is designed to provide high shape selectivity for separation of hydrophobic structurally related isomers and unique selectivity complementary to other reversed-phase columns. Acclaim C30 columns are available in 3 and 5μm particle sizes and 4.6, 3.0, and 2.1 mm diameters, with an average pore diameter of 200 Å. It is based on covalent modification of C30 alkyl silane on high-purity, spherical, poro…

The Thermo Scientific™ Acclaim™ Trinity™ P2 column is a high-performance, silica-based column specifically designed for separation of pharmaceutical mono- and multi-valent counterions by high-performance liquid chromatography (HPLC). This column was engineered for analytical chemists who need simple, robust, fast, generic methods. The Acclaim Trinity P2 columns provide an effective solution for counterion analysis using one co…

Use Thermo Scientific™ GlycanPac™ AXH-1 LC Columns for qualitative, quantitative, and structural characterization of uncharged and charged glycans present in biological molecules.This high-performance, silica-based high-performance liquid chromatography (HPLC) column provides simultaneous separation of glycans by charge, size and polarity. Achieve high-resolution and high-throughput analysis with unique selectivity for biologi…

Achieve exceptional peak shape and resolution for your HPLC and LC/MS applications with Thermo Scientific™ Hypersil GOLD™ HPLC columns. These endcapped, ultrapure, silica-based columns deliver significant reduction in peak tailing using generic gradients with C18 selectivity. With their excellent resolution, efficiency, and sensitivity, Hypersil GOLD columns give you confidence in the accuracy and quality of your analytical da…

For very hydrophobic analytes, achieve faster separations with excellent peak shapes using Thermo Scientific™ Hypersil GOLD™ C4 HPLC columns. Featuring a lower hydrophobicity than C18 or C8 columns, Hypersil GOLD C4 columns provide outstanding sensitivity and high efficiency.

Use the new Thermo Scientific™ MAbPac™ RP columns for high-performance reversed-phase (RP) HPLC and LC/MS characterization of monoclonal antibodies (mAbs), fragments, variants, antibody drug conjugates (ADCs), PEGylated proteins, and bispecific proteins. These columns have excellent resolution, long column lifetime, and low carry-over.

Utilize the increased separation speed and resolution of the Thermo Scientific™ Vanquish™ UHPLC system to detect and quantify virtually any substance using the near-universal detection capabilities of Vanquish Charged Aerosol Detectors. Ideal for everyday analyses, the Thermo Scientific™ Vanquish™ Charged Aerosol Detector F offers enhanced linear dynamic range and accurate, consistent response. This detector can measure low-le…

Combine maximum injection precision and huge sample capacity. The Thermo Scientific™ Vanquish™ Split Samplers employ high-throughput performance for the highest confidence in peak identification and quantitation possible. The samplers provide fully biocompatible fluidics, unsurpassed sample dosing precision, and is optimized for ruggedness and uptime, even under the toughest system pressure of up to 1500 bar (22,000 psi) and c…

Combine maximum injection precision and huge sample capacity. The Thermo Scientific™ Vanquish™ Split Samplers employ high-throughput performance for the highest confidence in peak identification and quantitation possible. The samplers provide fully biocompatible fluidics, unsurpassed sample dosing precision, and is optimized for ruggedness and uptime, even under the toughest system pressure of up to 1500 bar (22,000 psi) and c…

Improve productivity using the Thermo Scientific™ Vanquish™ Dual Split Sampler F. Designed to support Thermo Scientific™ Vanquish™ Duo UHPLC system for Dual LC, this unique biocompatible autosampler with 1000 bar pressure, the Vanquish Dual Split Sample F combines two injection devices in one housing, resulting in two separate channels that are individually controllable (injector volume, wash cycles, etc.).

Improve productivity using the Thermo Scientific™ Vanquish™ Dual Split Sampler H. Designed to support Thermo Scientific™ Vanquish™ Duo UHPLC system for Dual LC, this unique biocompatible autosampler with 1500 bar pressure, the Vanquish Dual Split Sample F combines two injection devices in one housing, resulting in two separate channels that are individually controllable (injector volume, wash cycles, etc.).

Employ an intelligent robotic that is fully integrated into a Thermo Scientific™ Vanquish™ UHPLC system with the Vanquish Charger module. With a capacity of 8832 samples (23 well plates), the Charger module automates sample loading into the Vanquish system for extra-long unattended operation. Environmental controls ensure reliable sample protection. A barcode reader automatically inventories each plate or rack within the devic…

Reduce the size and cost of your liquid chromatography (LC) system. The Thermo Scientific™ UltiMate™ ACC-3000 Autosampler Column Compartments provides a cost-effective solution for conventional LC and UHPLC applications at up to 62MPa (9000 psi) operating pressure. It integrates an autosampler and column compartment (with optional sample thermostatting) in a single module and offers performance and flexibility that exceed mos…

Simplify off-line multidimensional and multistep liquid chromatography (LC) in analytical and micro flow rate ranges. The Thermo Scientific™ UltiMate™ Autosampler with Fraction Collector uniquely supports both sample injection, fractionation, and reinjection using two integrated switching valves. The instrument fully automates multidimensional separations, allowing columns that differ in chemistry and internal diameter to be c…

Extend your biocompatible flow path for sensitive analyses. The Thermo Scientific™ Dionex™ UltiMate™ WPS-3000TBRS Biocompatible Rapid Separation Well Plate Autosampler protects your samples from start to finish with a ceramic needle, titanium injection valve and biocompatible fingertight fittings. This UHPLC autosampler uniquely injects up to 500µL at pressures up to 15,000 psi (103.4 MPa). The in-line split-loop (or flow-thro…

Obtain maximum application flexibility including Dual LC, Tandem LC or LC-MS, and Inverse Gradient workflows using the biocompatible Thermo Scientific™ Vanquish™ Dual Pump F. This pump provides two independent ternary-solvent blending flow streams in one housing. Our built-in Thermo Scientific™ SmartFlow™ pumping technology automatically compensates for changing eluent compressibility ensuring excellent flow and gradient preci…

Enjoy the flexibility of a quaternary pump with the fast gradient performance of a binary pump. The unique Thermo Scientific™ UltiMate™ LPG-3400XRS Extended Pressure Range Quaternary Pump is an LC/MS optimized quaternary UHPLC pump offering state-of-the-art performance for high speed and ultrahigh resolution applications. The compact pump head design, with a gradient delay volume of just 200µL, supports ballistic gradients for…

Extend the capabilities of the Thermo Scientific™ Vanquish™ UHPLC platform by adding all the flexibility you can expect from a state-of-the-art quaternary solvent blending. The biocompatible Thermo Scientific™ Vanquish™ Quaternary Pump F incorporates our SmartFlow™ pumping technology, which ensures excellent flow and gradient precision, independent of eluent composition and backpressures up to 1034 bar. This gives you unrivale…

For more mobile phase flexibility in any ultrahigh speed and ultrahigh resolution application, the Thermo Scientific™ UltiMate™ HPG-3400RS Rapid Separation Binary Pump offers more solvent capability. With an unrivaled flow-pressure footprint, the RSLC pump is the most flexible binary UHPLC pump available. The UltiMate 3000 Binary RS pumps are capable of providing true ballistic gradients with excellent retention time precision…

With exceptional flow stability and adjustable delay volume, the Thermo Scientific™ UltiMate™ HPG-3200RS Rapid Separation Binary Pump excels at high-speed and high-resolution applications. An industry-leading flow/pressure footprint of 100 MPa (15,000 psi) at up to 5mL/min. and 80 MPa (11,600 psi) at 8mL/min. makes it the most flexible binary pump choice. Designed with a stainless-steel-free (biocompatible) flow path, the HPG-…

Enjoy accurate gradients and precise retention times while meeting the requirements of diverse sample types with a wide range of flow rates. The Thermo Scientific™ UltiMate™ HPG-3200SD Standard Binary Pump is ideal for analytical LC separations on standard-bore columns. With support for pressures up to 62MPa (9,000 psi, 620 bar) at flow rates of up to 10 mL/min., UltiMate 3000 SD pumps excel in standard LC applications offer a…