High-throughput screening (HTS) is an automated drug discovery technique for identification of active compounds against a compound library. Use HTS readers and integrated assay preparation / analysis workstations to screen your compounds. Identify active compounds against various HTS libraries, including membranes, proteins and peptides and HTS cell lines. Find the best high-throughput screening products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
Cyclic GMP (Guanosine 3',5'-cyclic monophosphate) is a major second messenger that mediates cell activities by activating protein effectors such as protein kinase G (PKG), cGMP-regulated phosphodiesterases (PDEs), and cGMP-gated channels. Cellular cGMP is synthesized by soluble Guanylyl Cyclases and particulate Guanylyl cyclase, triggered by nitric oxide and atrial natriuretic peptide (ANP) binding respectively. Key processes…
Stable, recombinant AequoScreen ® parental cell line expressing aequorin and Gα16 in CHO-K1 host cells.
An acetyl-biotinylated substrate recognized by a Eu3+ anti-acetyl MAb and SA-XL665, which is used in activator screening or to test an High Throughput SIRT1 inhibitor. The level of enzymatic activity is inversely proportional to the amount of signal produced in the system. For more information please visit Cisbio Bioassays website.
In order to provide you with the most advanced tools for GPCR cell-based assays HTRF Technology now incorporates Lumi4®-Tb chemistry. Lumi4®-Tb is the first and only TR-FRET terbium cryptate, and is fully compatible with all existing HTRF readers. Therefore IP-One Tb offers new levels of sensitivity and performance in cell-based assays.
Cisbio Bioassays's cAMP HiRange kit is designed to address either the use of cell-based and cellular membranes-assays in GPCR activation and Adenylate Cyclase activity screening. The cAMP HiRange assay is highly robust and produces quality results while eliminating the need for a continuous supply of living cells. Features: Simple and rapid assay protocol Small quantity of membrane required per assay Signal to background ratio…
R&D Flow Cytometry Kit - Flow cytometry kits designed for analysis of cell populations use up to four fluorochrome-conjugated antibodies. Flow cytometry facilitates quantitative, multiparameter analysis of cell populations based on the expression of cell surface and/or intracellular molecules. R&D Systems offers a wide range of biotin-, fluorescein-, phycoerythrin-, or allophycocyanin-conjugated monoclonal antibodies specific…
R&D Prostaglandin E2 Parameter ELISA Kit - For the quantitative determination of Prostaglandin E2 (PGE2) in cell culture supernates, serum, plasma, and urine. The R&D Parameter PGE2 ELISA is a 3.5 hour forward sequential competitive enzyme immunoassay designed to measure PGE2 in cell culture supernates, serum, plasma, and urine. This R&D Paramerter assay is based on the forward sequential competitive binding technique in whic…
R&D Proteome Profiler Human Phospho-Kinase Array Kit - Human Phospho-Kinase Array Kit for the parallel determination of the relative levels of protein phosphorylation. The kit contains 4 sets of 2 membranes - each spotted in duplicate with 46 different kinase antibodies. The R&D Human Phospho-Kinase Array is a rapid, sensitive, and economical tool to simultaneously detect the relative levels of phosphorylation of 46 kinase ph…
R&D VEGF -Recombinant Human VEGF 165R Protein Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. Specifications and Use for R&D VEGF 165RSource of R&D VEGF• A DNA sequence encoding the 165 amino acid residue variant of human VEGF (Leung, D.W. et al., 1989, Science 246:1306) was expressed in…
R&D Activin - Recombinant Activin A Protein from Human/Mouse/Rat Activins and inhibins, members of the TGF-β superfamily, have been shown to have a wide range of biological activities including: mesoderm induction, neural cell differentiation, bone remodeling, hematopoiesis and reproductive physiology. Specifications and Use for R&D Activin ASource of R&D Activin• A DNA sequence encoding the human Activin A protein sequenc…
R&D Systems Recombinant Human BMP-4 BMP-4 is a TGF-β superfamily ligand that is widely expressed from early embryogenesis through adulthood. It plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair. Specifications and Use of R&D BMP-4R&D BMP-4 Source • A DNA sequence encoding the human BMP-4 mature chain (amino acid r…
R&D Recombinant Mouse Wnt-3a Wnt proteins are secreted glycoproteins that contain a conserved pattern of 23-24 cysteine residues. Wnts play critical roles in both carcinogenesis and embryonic development. Specifications and Use for R&D Wnt-3aSource of R&D Wnt-3a • A DNA sequence encoding the signal peptide from human CD33, Met 1 - Ala 16, joined with the mature mouse Wnt-3A, Ser 19 - Lys 352 (Accession # P27467; Roelink, H.…
R&D Systems Recombinant Human IL-4 Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa - 18 kDa Th2 cytokine that shows pleiotropic effects during immune responses. It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four α-helix structure. Specifications and Use of R&D Systems Recombinant Human IL-4Source • A DNA sequence en…
R&D TNF-alpha / TNFSF1A - Recombinant Human Protein Tumor necrosis factor alpha (TNF-alpha), also known as cachectin and TNFSF1A, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. Specifications and Use for R&D TNF-alphaSource of R&D TNF• A DNA sequence encoding the mature human TNF-α protein sequence (Accession…
R&D Systems Recombinant Human FGF basic (146 aa) FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35 - 60% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway. Specifications and Use of R&D FGF basic Source of R&D FGF • A DNA sequenc…
R&D Systems Recombinant Human IL-6 Interleukin 6 (IL-6) is a pleiotropic α-helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is essential for the transition from acute inflammation to either acquired immunity or chronic Specifications and Use of R&D IL-6 Source• A DNA sequence encoding the mature human IL-6 protein sequen…
R&D Systems Recombinant Human IGF-I/IGF-1, CF Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Human IGF-I is synthesized as two precursor isoforms with N- and alternate C-terminal propeptides. These isoforms are differentially expressed by various tissues. Specifications and Use of R&D Recombinant Human IGF-1 / ICF-1, CFSource…
R&D Systems Recombinant SCF Protein Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28 - 40 kDa type I transmembrane glycoprotein. It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors. Specifications and Use for R&D SC…
R&D Mouse TNF-alpha/TNFSF1A DuoSet This R&D DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse Tumor Necrosis Factor alpha (TNF-alpha). DuoSets are designed for the analysis of cell culture supernates. Other sample types, such as serum and plasma, need to be validated prior to use in this R&D DuoSet. Each R&D DuoSet ELISA kit cont…
R&D SCF - Recombinant Mouse Protein Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28 - 40 kDa type I transmembrane glycoprotein. It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors. Specifications and Use for R&D SC…
R&D Systems Recombinant Human LDL RThe low density lipoprotein receptor (LDLR) is the founding member of the LDLR family of scavenger receptors. This family contains transmembrane molecules that are characterized by the presence of EGF repeats, complement-like repeats, and YWTD motifs that form β-propellers. Specifications and Use for R&D LDL RSource of R&D LDL R• A DNA sequence encoding the signal peptide from human CD33 was…
R&D M-CSF - Recombinant Mouse Protein M-CSF, also known as CSF-1, is the primary regulator of macrophage survival, proliferation and differentiation and is essential for the survival and proliferation of osteoclast progenitors. M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis.…
R&D PDGF BB, CF - Recombinant PDGF-BB Human Protein Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the α-granules of platelets activated during the coagulation of blood to form serum. Specifications and Use of R&D PDGFSource of of R&D PDGF • A DNA sequence encoding the 109 amino acid residue mature human PDGF-B chain p…
R&D Systems - Recombinant Human Bone morphogenetic protein 2 (BMP-2) Bone morphogenetic protein 2 (BMP-2)is a member of the BMP subgroup of the TGF-β superfamily. It plays a dominant role in embryonic dorsal-ventral patterning, organogenesis, limb bud formation, and bone formation and regeneration.Specifications and Use of R&D BMP-2R&D BMP-2 Source • The DNA sequence encoding human BMP-2 (Wozney, J. et al., 1988, Science 242…
R&D Systems - Recombinant Human HGF (NS0-expressed)HGF, also known as Hepatopoietin A, was initially identified as a potent mitogen for hepatocytes in primary culture. Subsequently, HGF was also shown to be mitogenic for a variety of cell types including endothelial and epithelial cells, melanocytes, and keratinocytes. Specifications and Use of R&D HGFSource of R&D human HGF • A DNA sequence encoding the 728 amino acid residu…
