UHPLC and HPLC Products & Reviews

Developed to offer more pressure capabilities than ever before, the Thermo Scientific™ Vanquish™ Binary Pump H comes without any tradeoff on durability and robustness. From ultrafast to extremely shallow gradients at pressures up to 150 MPa, the industry-leading fourth generation Thermo Scientific™ SmartFlow™ pumping technology of the Vanquish system always provides you with unmatched retention time reproducibility and the low…

Having trouble resolving your compounds using your C18 column? The new Thermo Scientific Accucore Biphenyl LC column is your solution for: Aromatic & moderately polar analytes, Drugs of abuse, Steroids Now you can stop struggling with unresolved peaks, long run times, and poor lot-to-lot consistency. Obtain unique selectivity for aromatic and moderately polar analytes using Accucore Biphenyl LC Columns. These columns have opti…

Discover the unique Thermo Scientific™ ProPac™ Elite WCX weak cation exchange (WCX) liquid chromatography column. Designed for protein characterization including therapeutic proteins, monoclonal antibodies (mAbs), antibody drug conjugates (ADCs) and biosimilar proteins, this column offers high resolution, high efficiency separation of proteins and their charge variants. The unique column chemistry provides excellent perform…

Porous graphitic carbon (PGC, Hypercarb™) has unique properties as a stationary phase in high-performance liquid chromatography (HPLC). Its chemical surface properties distinguish it from more conventional LC packings such as bonded-silica gels and polymers. PGC behaves as a strongly retentive alkyl-bonded silica gel for non-polar analytes. However, its retention and selectivity behavior towards polar and structurally-related…

Ensure robust nano and capillary flow LC-MS with Thermo Scientific™ EASY-Spray™ LC Columns. The integrated column/emitter design eliminates dead volume and is temperature-controlled for maximum reliability and performance. Rigourously tested to ensure maximum quality, these columns are designed for maximum simplicity and ease-of-use.

For prep HPLC columns, one size does not fit all. Whether you need unique chemistries for difficult separations to purify your compound from impurities and side products, robust columns for longer use, or budget-friendly solutions for routine separations, our preparative column portfolio delivers. Our full range of column hardware with optional XtendedLife technology offers robust and reproducible performance along with a wide…

With YMC-Triart Prep, pH stable preparative HPLC stationary phases are available for industrial-scale applications. This hybrid silica-based material can be used in a pH range of 2.0 - 10.0 and allows more efficient cleaning procedures. The innovative YMC-Triart Prep combines mechanical strength and pH stability.

EASY-Spray fully integrated HPLC column/temperature/emitter design. Glass emitter (purchased separately) delivers an exceptionally stable spray. Integrated column with temperature control provides maximum reliability and performance. A single nanoViper connection between the HPLC column and the MS ion source eliminates dead volumes. Thermo Scientific™ nanoViper™ fingertight fitting prevents overtightening and bad connections.…

Use the Thermo Scientific™ MAbPac™ Capillary Reversed Phase HPLC Column high sensitivity HPLC or LC/MS characterization of intact proteins in top-down proteomics applications where sample amount is critically limited. This versatile column can be used for peptide-mapping characterization where the capillary format balances sensitivity with throughput. 

For sensitive protein and peptide separation using nano and capillary HPLC. These emitters offer easy connection to Thermo Scientific™ MAbPac™ and Accucore™ nano and capillary columns for enhanced separation and MS detection of proteins, peptides and monoclonal antibodies (mAbs).

Employs the unique and universal Charged Aerosol Detector to consistently quantify all non-volatile and semi-volatile compounds even when no reference standard is available. Improve your lab's quantification capabilities with the Thermo Scientific Vanquish Duo HPLC and UHPLC Systems for Inverse Gradient.

 Increase your throughput and maximize detector output using a second pump and intelligent column switching to eliminate the time required for column equilibration in gradient elutions. The Thermo Scientific Vanquish Duo HPLC and UHPLC Systems for Tandem LC or LC-MS increase sample throughput for any LC or LC-MS analysis.

Double your throughput and deepen your sample analysis using the Thermo Scientific Vanquish Duo UHPLC System for Dual LC. Features a dual split sampler and two pumps and flow paths, allowing you to run the same method or two different methods simultaneously on the same instrument, maximizing your lab productivity and return on investment.

Perform analyte pre-concentration and sample clean-up using an automated, repeatable workflow while saving your solvent usage and time compared to offline sample preparation steps. Ensure confidence in your complex sample analysis with the Thermo Scientific Vanquish Online SPE HPLC and UHPLC Systems.

Heart-Cut 2D-LC can reveal hidden impurities that co-elute with the analytes of interest by using two complementary column chemistries to gain more separation power. Increase your confidence in peak purity using the Thermo Scientific Vanquish Loop Heart-Cut 2D-LC System.  

Achieve in-depth analysis for greater confidence in peak purity and identity with the Thermo Scientific Vanquish Trap Heart-Cut 2D-LC System. This two-dimensional LC (LC-LC) system enables you to collect a single fraction of the first-dimension separation onto a trap column to pre-concentrate or achieve mobile phase compatibility for UV and MS detection.

This two-dimensional LC (LC-LC) system is an innovative, all-in-one system that uses the novel Thermo Scientific Vanquish Dual Split Sampler to enable you to switch between both system capabilities without re-plumbing. Seamlessly switch between a trap heart-cut 2D-LC system and a dual LC system using the innovative Thermo Scientific Vanquish Simple Switch 2D-LC System. 

Accelerate and automate your method development using Thermo Scientific Vanquish Method Development HPLC and UHPLC systems. These systems optimize separation conditions by testing thirteen different eluents on up to six HPLC columns. Achieve fully automated method development with ChromSwordAuto Chromeleon Connect and ChromSword AutoRobust Chromeleon Connect. 

BioPro IEX and BioPro IEX SmartSep resins are specifically designed for biochromatography. They are available as a strong anion exchanger (BioPro IEX Q) and a strong cation exchanger (BioPro IEX S). The resins are based on a hydrophilic polymer matrix, with a particle size of 10 µm, 20 µm, 30 µm and 75 µm.

Highly robust hybrid silica material for the purification of proteins, peptides and oligonucleotides, which combines mechanical strength and pH stability. The larger pore size makes YMC Triart-Prep Bio200 C8 the ideal choice for the purification of mid-size to large biomolecules.

Experience compelling fraction purity with the Thermo Scientific™ Vanquish™ Fraction Collector (VFC) using innovative valve technology for enhanced resolution, product recovery, and minimized carry-over.

Experience the high-resolution fractionation of the Thermo Scientific™ Vanquish™ Analytical Purification LC Systems featuring the integrated Thermo Scientific™ Vanquish™ Fraction Collector. 

Markes’ stainless sorbent tubes are available in two sizes: 3.5” x ¼” (industry-standard), and 4.5” x 6mm (DAAMS). Industry-standard tubes are compatible with Markes, PerkinElmer, Shimadzu, Dani, CDS and Gerstel 3.5+ thermal desorption (TD) systems.

The Ultra line represents Restek’s broadest selection of stationary phases on a single silica support.Made of high-purity, type-B silica that minimizes activity and creates high-density bonding, the Ultra Amino Columns (USP L8) are designed for selective and reliable HPLC applications. Particle size: 3 μm or 5 μm, spherical Pore size: 100 Å Carbon load: 2% End-cap: no pH range: 2.5 to 8 Temperature limit: 80 °C USP phase code…

The Ultra line represents Restek’s broadest selection of stationary phases on a single silica support. Made of high-purity, type-B silica that minimizes activity and creates high-density bonding, the Ultra Cyano Columns (USP L10) are designed for selective and reliable HPLC applications.Particle size: 3 μm or 5 μm, spherical Pore size: 100 Å Carbon load: 8% End-cap: yes pH range: 2.5 to 8 Temperature limit: 80 °C USP phase co…