ELISA Reagents and Buffers Products & Reviews

The Unconjugated Estriol Enzyme Immunoassay (EIA) Kit provides materials for the quantitative measurement of unconjugated estriol in serum.

Gyrolab huIgG Kit – High Titer is a ready-to-use Gyrolab kit for determination of human monoclonal antibody (mAb) titers up to 1 mg/mL.

Gyrolab   E. coli HCP Kit is a ready-to-use Gyrolab kit for detection of HCPs from Escherichia coli (E. coli) expression systems.

Gyrolab Protein A Kit is a ready-to-use Gyrolab kit for detection of residual Protein A ligands in bioprocess samples. 

Gyrolab Generic PK Kit  is a ready-to-use Gyrolab kit for quantification of human antibody biotherapeutics in pharmacokinetic (PK) studies performed in non-human serum matrices during preclinical development.

Gyrolab Assay Toolbox for development of HCP assays with the components of ready-to-use HCP Kits. 

Gyrolab Mixing CD 96 is recommended when applications require a pre-treatment step, e.g., acid dissociation prior to analysis of anti-drug antibodies. 96 datapoints.

Gyrolab huIgG Kit – Low Titer is a ready-to-use Gyrolab kit for determination of human monoclonal antibody (mAb) titers up to 20 µg/mL.

Gyrolab 2G Generic PK Kit enables the quantification of human therapeutic antibodies in non-human matrices during pre-clinical PK studies in early-stage biotherapeutic development. 

TMB, (3,3',5,5'-tetramethybenzidine) has been shown to be a safe-sensitive substrate for the assay of HRP. Initially, in the presence of HRP and hydrogen peroxide, a one-electron oxidation product is formed. This compound, a cation free radical, is blue in color with an adsorption maximum of 653 nm. Further reaction with HRP/H2O2 or acidification of the radical with acid yields the diimine terminal oxidation product adsorbing…

The Luminata ELISA HRP chemiluminescent substrates provide three major advantages:High sensitivity for detecting as little as 0.1 pg of proteinBroad dynamic detection range (up to 2,500-fold), enabling a wide range of quantitation.Flexibility to adjust ELISA signal strength

Neptune™ HRP Conjugate Stabilizer preserves the activity of HRP-protein conjugates. This HRP stabilizer can be used to store concentrated stock HRP conjugates, reconstitute lyophilized conjugates, and dilute HRP conjugated antibodies to their useful working titer in ELISA tests and immunology-based techniques.Neptune™ HRP Conjugate Stabilizer is especially suited for ELISAs using anti-IgG HRP conjugates within traditional ant…

General Block contains a mammalian protein blocking agent suitable for most antibody capture ELISA formats and peptide or protein antigen-down ELISA formats. General Block contains a mammalian protein blocking agent designed to reduce nonspecific background noise in most antibody capture ELISA formats and peptide/protein antigen-down ELISA formats. This unique, low-level blocking buffer minimizes nonspecific binding interacti…

Neptune Block Blocking Buffer is designed primarily for antigen-down ELISA formats as well as sandwich assays with high background problems. Neptune Block ELISA Blocking Buffer is particularly useful when working with human and other mammalian serum samples as it works to reduce interactions between sample and blocking molecules.Neptune Block ELISA Blocking Buffer is a mixture of small molecular proteins and molecular stabili…

Eliminate interference and nonspecific background noise associated with antibody-coated ELISA formats and sandwich ELISAs. SynBlock ELISA Blocking Buffer provides superior background-blocking performance without the use of conventional cross-reactive protein additives. SynBlock ELISA Blocking Buffer is a novel non-protein blocking formulation designed to eliminate interference and nonspecific background noise associated with…

Phosph-Free Block ELISA Blocking Buffer is a novel non-protein blocking formulation designed to eliminate interference and nonspecific background noise associated with antibody-coated ELISA formats and sandwich ELISAs.Phosph-Free Block is formulated for ELISAs using alkaline phosphatase detection or for assays with ultra-sensitivity requirements. However, it is appropriate for assays using HRP detection as well. Using inert no…

Reduce non specific binding in ELISAs and stabilize proteins with Alternative Block, an all-synthetic ELISA blocking buffer free of proteins and detergents. Alternative Block is a protein-free, detergent-free ELISA blocking buffer. This unique blocking buffer contains a heterogeneous mixture of proprietary blockers and synthetic stabilizers that block the uncoated regions of the microtiter plate without the use of conventiona…

Reduce non specific binding in ELISAs, address high background issues, and stabilize proteins with Monster Block, a new ELISA blocking buffer utilizing a heterogenous mixture of nonmammalian blocking agents. Monster Block™ provides a high degree of blocking efficiency and protein stabilization through the use of non-mammalian protein blocking agents and stabilizers. The heterogeneous composition of this unique ELISA blocking…

Peroxidase labelling of proteins or amine compounds.  Only 3 hours to get conjugates, all processes in a single filtration tube, high recovery of conjugates.

The Quant*Tag™ Biotin Kit (BDK-2000) is designed to determine the amount of free biotin in solution or the number of biotins attached to nucleic acids, proteins, or other macromolecules. This kit can be used to determine accurately the labeling efficiency of biotin-labeled molecules.

Vector Laboratories’ affinity-purified antibodies are of unmatched quality. These antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques.

Vector Laboratories' fluorescent secondary antibodies are unmatched in quality for immunological techniques. All antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies.

SuperSignal ELISA Femto Substrate is formulated for superior protein detection and low-end linearity in chemiluminescent ELISA applications.

Pierce TMB ELISA Substrate is a popular chromogenic ELISA substrate for the detection of horseradish peroxidase

TMB One Component HRP Microwell Substrate