Nuclease Products & Reviews
Nuclease
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Benzonase® Nuclease, ultrapure
Merck KGaA, Darmstadt, Germany99% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution, ultrapure grade
Nuclease P1 from Penicillium citrinum
Merck KGaA, Darmstadt, Germanylyophilized powder, ≥200 units/mg protein (E1%/280, 3′-5′-Phosphodiesterase)
Ribonuclease A from bovine pancreas
Merck KGaA, Darmstadt, Germanyfor molecular biology, ≥70 Kunitz units/mg protein, lyophilized
Lentiviral Cas9 nuclease reagents
Lentiviral CRISPR-Cas9 components for robust gene editing in biologically relevant cell types
PURedit™ Protein
MerckRecombinant PURedit Cas9 protein from Streptococcus pyogenes is a ready-to-use reagent for genome engineering experiments
Cas9 Plus Protein
MerckRecombinant Cas9 Plus protein from Streptococcus pyogenes is a ready-to-use reagent for genome engineering experiments.
Cas9-GFP Protein
MerckRecombinant Cas9-GFP protein from Streptococcus pyogenes is a ready-to-use reagent for genome engineering experiments.
Transcreener dAMP Exonuclease Assay
BellBrook LabsThe Transcreener dAMP Exonuclease Assay directly measures dAMP produced by exonuclease enzymes as they cleave polynucleotides.
Exonuclease I
Thermo Fisher ScientificThermo Scientific Exonuclease I digests single-stranded DNA 3' to 5' (requires Mg2+ and a free 3'-OH). The nuclease is inactivated by heating at 80°C for 15 minutes.
Shrimp Nuclease
Thermo Fisher ScientificThermo Scientific Shrimp Nuclease is an endonuclease that cleaves phosphodiester linkages in DNA to yield di- and oligonucleotides with 5’-phosphate and 3’-hydroxyl termini. The enzyme has a high preference for double-stranded DNA, and can be used to selectively degrade dsDNA, leaving ssDNA and RNA intact. The enzyme is inactivated after 15 minutes at 65 °C.
RNase A
Thermo Fisher ScientificThermo Scientific RNase A (ribonuclease A) is supplied as a chromatographically pure freeze-dried powder, salt-free for easy reconstitution in most buffers.
RNase H
Thermo Fisher ScientificThermo Scientific Ribonuclease H (RNase H) is an endonuclease that degrades the RNA portion of DNA-RNA hybrids. It hydrolyses phosphodiester bonds of RNA in RNA:DNA heteroduplexes. It produces products with 3’-OH and 5’-phosphate. RNase H will not degrade single or double-stranded DNA or RNA.
PowerCut™ Dicer
Finnzymes OyFinnzymes’ novel PowerCut™ Dicer produces a pool of 25–27 nucleotide-long siRNA molecules from any dsRNA. This efficient endoribonuclease originating from Giardia intestinalis outperforms all other siRNA producing enzymes by cleaving the substrate with 100 % efficiency yet leaving the resulting siRNA intact. The high quality siRNA produced is ideal for gene silencing studies, for example. Pooled siRNAs are also preferred over…