Nuclease Products & Reviews

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PURedit™ Protein

Merck

Recombinant PURedit Cas9 protein from Streptococcus pyogenes is a ready-to-use reagent for genome engineering experiments

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Cas9 Plus Protein

Merck

Recombinant Cas9 Plus protein from Streptococcus pyogenes is a ready-to-use reagent for genome engineering experiments.

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Cas9-GFP Protein

Merck

Recombinant Cas9-GFP protein from Streptococcus pyogenes is a ready-to-use reagent for genome engineering experiments.

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Shrimp Nuclease

Thermo Fisher Scientific

Thermo Scientific Shrimp Nuclease is an endonuclease that cleaves phosphodiester linkages in DNA to yield di- and oligonucleotides with 5’-phosphate and 3’-hydroxyl termini. The enzyme has a high preference for double-stranded DNA, and can be used to selectively degrade dsDNA, leaving ssDNA and RNA intact. The enzyme is inactivated after 15 minutes at 65 °C.

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RNase A

Thermo Fisher Scientific

Thermo Scientific RNase A (ribonuclease A) is supplied as a chromatographically pure freeze-dried powder, salt-free for easy reconstitution in most buffers.

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RNase H

Thermo Fisher Scientific

Thermo Scientific Ribonuclease H (RNase H) is an endonuclease that degrades the RNA portion of DNA-RNA hybrids. It hydrolyses phosphodiester bonds of RNA in RNA:DNA heteroduplexes. It produces products with 3’-OH and 5’-phosphate. RNase H will not degrade single or double-stranded DNA or RNA.

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PowerCut™ Dicer

Finnzymes Oy

Finnzymes’ novel PowerCut™ Dicer produces a pool of 25–27 nucleotide-long siRNA molecules from any dsRNA. This efficient endoribonuclease originating from Giardia intestinalis outperforms all other siRNA producing enzymes by cleaving the substrate with 100 % efficiency yet leaving the resulting siRNA intact. The high quality siRNA produced is ideal for gene silencing studies, for example. Pooled siRNAs are also preferred over…

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