Nuclease Products & Reviews

DNase I, (RNase-free) is an endonuclease that nonspecifically cleaves DNA to release di-, tri- and oligonucleotide products with 5´-phosphorylated and 3´-hydroxylated ends (1,2). DNase I acts on single- and double-stranded DNA, chromatin and RNA:DNA hybrids.

HIghlights 100 T Equivalent: Prepared from Arthrobacter lutes. Essential enzyme activities are β-1,3-glucanase and β-1,3-glucan laminaripentao-hydrolase. Convenient: Provided lyophilized along with a storage buffer for reconstitution. Efficient Cell Wall Digestion: Supplied storage buffer has been optimized to confer maximum levels of enzymatic activity. Digestion of yeast and fungal cell walls is necessary for…

Detect microbial deoxyribonuclease enzymes, particularly from staphylococci, with Thermo Scientific™ Oxoid™ DNASE Agar (Dehydrated).

Thermo Scientific rDNase I is produced through a process that contains no animal by-products. The enzyme is provided free from any detectable RNase activity or animal derivatives. rDNase I has a molecular weight of approximately 30kDa. As an endodeoxyribonuclease, DNase I hydrolyses phosphodiester linkages yielding di-, tri-, and oligonucleotides with a 5' phosphate and a free 3' hydroxyl. DNase I has been shown to act on sing…

Properties: Endoribonuclease that preferentially cleaves 3' to G. Guanosine-3'-phosphates and oligonucleotides with guanosine-3'-phosphate terminal groups are the final products. Substrate is single-stranded RNA. Source: Aspergillus oryzae Volume activity: approx. 105 U/ml Unit definition: One unit according to Egami releases a sufficient quantity of acid-soluble oligonucleotides to produce an absorbance increase of 1.0 at 260…

Recombinant, high-purity endonucleases for genome editing experiments

lyophilized powder, Protein ≥85 %, 

Salt Active Nuclease High Quality (SAN HQ) is a Bioprocessing Grade nuclease available in GMP grade developed as the most efficient solution for removal of both single and double stranded DNA and RNA at high salt conditions(400–700 mM). It excels in DNA removal for robust viral vectors like Adenovirus and AAV, ensuring superior product purity while improving DSP. Ideal for gene therapy and vaccine manufacturing. Triton free av…

Double-Strand Specific dsDNase from ArcticZymes is ideal for removing contaminating DNA from PCR master mixes, preventing false positives in bacterial typing. Recombinantly produced in Pichia pastoris, it cleaves dsDNA with 5000-fold higher specificity than ssDNA. Easily inactivated by moderate heat (65°C), it allows for direct addition of DNA templates post-cleanup, ensuring accurate PCR results.

HL-SAN efficiently removes nucleic acids from buffers in protein purification, excelling in high-salt conditions to reduce aggregation. Ideal for removing nucleic acids from proteins with high DNA/RNA affinity, it performs well during lysis, early purification stages, and high-salt eluates. This cold-adapted enzyme works effectively at ambient temperatures and during overnight digestion at 4°C. Triton free available.