Protein Purification Products & Reviews

Protein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Trypsin/Lys-C Mix, Mass Spec Grade

Promega Corp.

Trypsin/Lys-C Mix, Mass Spec Grade, is a mixture of Trypsin Gold, Mass Spectrometry Grade, and rLys-C, Mass Spec Grade. The Trypsin/Lys-C Mix is designed to improve digestion of proteins or protein mixtures in solution.   Using the conventional trypsin digestion protocol (i.e., overnight incubation at nondenaturing conditions), Trypsin/Lys-C Mix improves protein digestion by eliminating the majority of missed cleavages,…

5.0/5.0
|2 Reviews
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Positive Pressure Manifold

UCT, Inc.

Positive Pressure Manifold - A simple, robust way to quickly process up to 48 samples at one time. The Positive Pressure Manifold can accommodate a multitude of column sizes and provides even flow to each position. Positive Pressure Manifold Features: All 48 positions are individually regulated to provide even pressure to each column. There are four banks of 12 positions each. The user can control flow to each of the four…

4.9/5.0
|3 Reviews
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Presston 1000

Phenomenex Inc

Positive pressure 96-well plate sample processing with Presston 1000 offers an advanced alternative to traditional vacuum manifold processing. To offer a wider applicability, Presston 1000 is equipped for processing samples in both a low pressure setting for typical solid phase extraction steps and a high pressure setting for very viscous sample processing and for sample dry down.  In addition, Presston 1000 is compatible with…

5.0/5.0
|1 Review

Protein A Agarose

Merck

Beads containing sufficient covalently-linked Protein A for a binding capacity of 20+/-2 milligrams human IgG per 1 milliliter.

5.0/5.0
|1 Review


GFP-Trap®

Chromotek GmbH

GFP -Trap® - use your fluorescence fusion as tag for biochemical studies.Curiously GFP, the most widely used labeling tag in cell biology, is rarely used for biochemical analyses. This may be due in part to limited GFP antibody availability and specificity as well as co-eluted heavy and light antibody chains that interfere with subsequent analyses.Our GFP-Trap® is a high quality GFP-binding protein coupled to a monovalent matr…

5.0/5.0
|2 Reviews

Thermo Scientific™ Pierce™ M-PER™ Mammalian Protein Extraction Reagent

Thermo Fisher Scientific

For highly efficient total protein extraction from cultured mammalian cells. The complete cell lysis reagent is a nondenaturing detergent formulation that dissolves cell membranes and extracts total soluble cellular protein in only 5 minutes. M-PER Reagent requires little or no mechanical disruption and yields more protein than freeze/thaw cycles and sonication.This mammalian cell lysis reagent is so efficient that adherent c…

4.7/5.0
|1 Review

Bio-Monolith Protein A and Protein G

Agilent Technologies

Agilent Bio-Monolith Protein A and Protein G LC columns are designed for analytical separation of all IgG subclasses (human and mouse). They deliver fast and accurate quantitation of mAb titer and purification of small amounts of mAb for further characterization.

4.7/5.0
|1 Review

Thermo Scientific NE-PER Nuclear and Cytoplasmic Extraction Kit

Thermo Fisher Scientific

Prepare concentrated nuclear extracts for an EMSA and gel shift assays. The Thermo Scientific NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours. The NE-PER Kit is a nuclear protein extraction method that involves simple, stepwise lysis of cells and centrifugal isolation of nuclear and cytoplasmic protein f…

4.5/5.0
|4 Reviews