Cell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.
Kit for the detection and quantification of apoptotic cell death on a single-cell level by flow cytometry and fluorescence microscopy, and for double labeling with fluorescein-labeled cell markers (TMR red)Benefits Convenient: No secondary detection system required. Accurate: Identification of apoptosis at a molecular level (DNA-strand breaks) and identification of cells at the very early stages of apoptosis. Flexible:…
Application The Cellular DNA Fragmentation ELISA may be applied to measure: Apoptosis by detection of BrdU-labeled DNA fragments in the cytoplasm of affected cells (= nonradioactive alternative to the [3H]-thymidine-based DNA-fragmentation assay). Cell-mediated cytotoxicity by detection of BrdU-labeled DNA fragments released from damaged target cells into the culture supernatant (= nonradioactive alternative to the [3H]-thymid…
Application The kit is used for the detection of BrdU incorporated into cellular DNA by immunocyto/histochemistry. Benefits Safe: No radioisotopes are used. Easy to perform: Follows a standard immunohistochemistry protocol. Sensitive: Denaturation of DNA with nucleases allows for highly sensitive detection of BrdU. Flexible: Allows double-labeling protocols.
Application The kit is used for the detection of BrdU incorporated into cellular DNA using immunofluorescence microscopy. It is used for the detection of DNA synthesis by either in vitro labeling of cells or organ cultures, or by in vivo labeling, in which frozen or paraffin-embedded tissue sections must be prepared prior to fixation. Benefits Safe: No radioisotopes are used. Easy to perform: Follows a standard immunof…
Application The Cell Proliferation ELISA, BrdU (colorimetric) belongs to the second, improved generation of kits for measuring DNA synthesis. It is designed as a precise, fast, and simple colorimetric alternative to quantitate cell proliferation based on the measurement of BrdU incorporation during DNA synthesis in replicating (cycling) cells. Thus, the Cell Proliferation ELISA can be used in many different in vitro cell syst…
ApplicationThe In Situ Cell Proliferation Kit, FLUOS belongs to the second generation of improved kits for the detection of DNA synthesis at the single-cell level (in situ) by immunocyto-/immunohistochemistry. The kit can be used in many different in vitro and in vivo cell systems, for example: Detection and quantification of cells in S-phase Determination of growth fractions for tumors in animal-model systems Detectio…
ApplicationThe immunoreactivity of the M30 antibody is confined to the cytoplasm of apoptotic cells. It can be easily used for: Immunocytochemistry Flow Cytometry Immunohistochemistry: paraffin-embedded tissue sections (secondary enhancer reagents needed for this particular application) and cryostat sections Benefits Determine caspase activity, even in formalin-embedded tissue. Identify the stage of apoptosis (…
Quantitative determination of Hu-IFN alpha bioactivity Sample Volume: 25 µl Catalog Number: 51100-1 Quantity: 1 x 96 Assay Range: 1.5-200 IU/ml Shipping Conditions: Dry ice Storage temp.: -80˚C Shelf Life: 9 months from date of manufacture 2007 Retail Price: $895
CDP-Star® and CSPD® chemiluminescent substrates for alkaline phosphatase let you detect alkaline phosphatase and alkaline phosphatase-labeled molecules with unparalleled sensitivity, speed, and ease.
Chemiluminescent substrate concentrates for E.coli ß-galactosidase assays.
Sapphire-II™ and Emerald-II™ chemiluminescence enhancers significantly reduce aqueous quenching and tune the wavelength of light emission.
In 2008 Gentronix launches a new S9 protocol to extend the application of the GreenScreen HC assay to include the detection of genotoxic metabolites. Many pharmaceuticals only present a genotoxicity hazard after metabolism and such chemicals are called pro-mutagens or pro-genotoxins. Generally, although not exclusively, the liver is considered responsible for the “metabolic activation” (MA) of promutagens. However, none of t…
Stable, recombinant AequoScreen ® parental cell line expressing aequorin and Gα16 in CHO-K1 host cells.
Real-Time qPCR assays for kinases and related genes reformatted onto an OpenArray plate. Kit includes: OpenArray plate, Real-Time qPCR Case, Immersion Fluid, Frame Adhesive and Plate-File CD.
Real-Time qPCR assays for Signal Transduction and related genes reformatted onto an OpenArray plate. Kit includes: OpenArray plate, Real-Time qPCR Case, Immersion Fluid, Frame Adhesive and Plate-File CD.
An acetyl-biotinylated substrate recognized by a Eu3+ anti-acetyl MAb and SA-XL665, which is used in activator screening or to test an High Throughput SIRT1 inhibitor. The level of enzymatic activity is inversely proportional to the amount of signal produced in the system. For more information please visit Cisbio Bioassays website.
The Iso-Gold kit is a rapid-format isotyping kit that reduces the labor of the traditional ELISA kit from a day-and-a-half procedure to a five-minute assay. It determines the isotype of your monoclonal antibody, assess the purity of your purified monoclonal antibody*, verify the purity of your purchased monclonal antibody* and reduces runtime. *multiple lines appear and indicate subclass contamination
The mTRAQ™ Reagents are duplex amine-specific, stable isotope labeled reagents designed for high confidence protein and peptide biomarker verification using MRM analysis on the QTRAP® system and the triple quadrupole mass spectrometers. * Duplex mass difference tags (Non-Isobaric Amine Tags) * Established chemistry with > 95% labeling efficiency * High selectivity MRM based relative quantitation of proteins/peptides/PTMs * Sup…
Osteoprotegerin (OPG) EIA is an enzyme immunoassay intended for the quantitative determination of OPG in plasma, serum or cell culture supernatants. OPG is a dimeric glycoprotein of the tumor necrosis factor (TNF) receptor family that acts as a soluble receptor for sRANKL (soluble receptor-activated NF-kB ligand) and prevents it from binding to and activating RANK, thus inhibiting osteoclast development. Possible indications f…
The Ampli-sRANKL EIA is an enzyme immunoassay intended for the quantitative determination of human free soluble RANK-Ligand in plasma, serum and cell culture supernatants. Format:ELISA / EIA, Sample Type:Serum, Plasma, Cell Culture, Sample Size:100 µl, Size:96 Wells, Range:0.125 - 2 pmol/l, Sensitivity:0.02 pmol/l, Incubation Time:Overnight, 1 hour 30 minutes
Oxidized LDL (Ab) EIA is a enzyme immunoassay intended for the quantitative determination of human IgG autoantibodies to oxidized low density lipoprotein (LDL) in serum. Format:ELISA / EIA, Sample Type:Serum, Sample Size:50 µl, Size:96 Wells, Range:37 - 1200 mU/ml, Sensitivity:27 mU/ml, Incubation Time:2 hours 20 minutes
