Molecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
TaqMan® MicroRNA Assays quantitate miRNAs with the specificity and sensitivity of TaqMan® Assay chemistry. A simple, two-step protocol requires only reverse transcription with a miRNA-specific primer, followed by real-time PCR with TaqMan® probes. The assays target only mature miRNAs, not their precursors, ensuring biologically relevant results. Get gold standard TaqMan Assay performance for miRNA quantitation and profiling…
The TaqMan® MicroRNA Reverse Transcription Kit provides the necessary components for optimal performance using Applied Biosystems TaqMan® MicroRNA Assays. Components of this kit are used with the RT primer provided with the TaqMan MicroRNA Assay to convert miRNA to cDNA.
TaqMan® PreAmp Pools are pre-pooled sets of Applied Biosystems TaqMan® Gene Expression Assays ready for use with four specific TaqMan® Gene Signature Arrays. TaqMan PreAmp Pools are designed for challenging RNA samples, such as very small quantities, or degraded material and genes that have low expression levels. Use PreAmp Pools to increase the quantity of your sample targets without introducing bias. Four pools are availab…
The TaqMan Sample-to-SNP Kit provides a streamlined protocol for performing TaqMan® chemistry-based genotyping analysis from any sample with a single kit. The kit is comprised of two parts: the DNA Extract All Lysis Reagents and the TaqMan® GTXpress™ Master Mix. The DNA Extract All Lysis Reagents reduce prolonged procedures for the release of real-time PCR-ready DNA to a 5 minute protocol. They can process a wide variety of…
Optimized to achieve over 50% transfection efficiency without sacrificing cellular health, each kit includes an optimized protocol, Prostate Reagent and Prostate Boost Reagent in sufficient quantities to conduct up to 500 transfections in 35mm wells.Features include:• Reduces levels of cell damage when compared to liposmal-based transfection reagents • Transfection can be conducted in serum-containing media to save time and el…
OpenArray® Pathways are ready-to-use gene panels containing pre-validated real-time qPCR assays. The plates have over 600 assays each and are compatible with multiple sample types. Panels are currently avaliable with human or mouse genes. New panels are continually under development. Check back for updates. Real-Time qPCR assays for Mouse Inflammation and related genes reformatted onto an OpenArray plate. Kit includes: OpenArr…
OpenArray® Pathways are ready-to-use gene panels containing pre-validated real-time qPCR assays. The plates have over 600 assays each and are compatible with multiple sample types. Panels are currently avaliable with human or mouse genes. New panels are continually under development. Check back for updates. Real-Time qPCR assays for Mouse Transcription Factors and related genes reformatted onto an OpenArray plate. Kit includes…
febit offers a broad variety of Geniom® Biochips useable with a Geniom RT Analyzer or febit’s Analytical Services. Customers can choose from the growing portfolio of Geniom Biochips or order Custom Geniom Biochips manufactured exactly to precise specifications. Geniom Biochips are available with validated and optimized probe design for specific applications such as: • microRNA Profiling • mRNA Profiling • HybSelect, sequence c…
The biochip from febit enables up-to-date profiling and enrichment of genes and SNPs of interest using Gene Expression Profiling, SNP-Detection, ncRNA analysis as well as targeted enrichment of genes for Next-Generation Sequencing (NGS).
TaqMan® Protein Expression Assays help quantitate protein expression using gold standard TaqMan® 5' nuclease chemistry with a workflow and sample quantity similar to our TaqMan® Gene Expression and MicroRNA (miRNA) Assays. As the protein expression results are obtained on the same analytical platform, these revolutionary assays enable direct correlation of mRNA and/or miRNA expression to protein expression.
Roche NimbleGen provides a unique combination of high-density arrays, long-oligo probes, and flexible design capability that together provide superior results for advanced gene expression analysis. NimbleGen microarrays enable accurate, sensitive, and specific interrogation of genome-wide expression for any sequenced and annotated genome. Researchers also can tile through a genome at any desired spacing to discover genome-wide…
MATra-si Reagent for efficient transfection of siRNA/miRNA as well as Antisense Oligos (ODN) using IBA's Magnet Assisted Transfection technology (MATra). MATra is a very gentle, easy-to-handle, fast and highly efficient technology to transfect cells in culture. Data from a variety of species using cell lines or primary tissue culture have accumulated including human, monkey, mouse, rat, xenopus, pig, cat or fish. Applications…
MATra-A Reagent for efficient transfection of plasmid DNA using IBA's Magnet Assisted Transfection technology (MATra). MATra is a very gentle, easy-to-handle, fast and highly efficient technology to transfect cells in culture. All types of nucleic acids from plasmid DNA or siRNA to oligonucleotides can be used with the MATra approach. Data from a variety of species using cell lines or primary tissue culture have accumulated in…
Test set for comparison of Magnet Assisted Transfection versus Magnet Assisted Lipofection. Including a one-month-rent of a universal magnet plate. Optimized for siRNA transfections. Magnet Assisted Transfection (MATra) is a new, easy-to-handle, very fast and highly efficient technology to transfect cells in culture. Data from a variety of species using cell lines or primary tissue culture have accumulated including human, mon…
TransIT®-LT1 Transfection Reagent from Mirus Bio- A broad spectrum, low toxicity, DNA transfection reagent • Broad Spectrum DNA Delivery - Utilize one transfection reagent and protocol for a variety of cells. • Low Cellular Toxicity - Maintain cell density and reduce experimental biases. • High Efficiency Delivery - Achieve expression in a large population of cells for experimental success. • Deliver Single or Multiple Plasmi…
The Recording Nanoject II: Enables High Precision Electrophysiological Guidance of Nanoliter Injections into the Brain Features of the Drummond Scientific Recording Nanoject II include:• Facilitates connection to an extracelluar amplifier to allow recording of neural activity • Supplements stereotaxic coordinates in guiding the placement of small injections • Provides direct displacement of neuronal tracer or excitotoxin fro…
The AccuPower® DNA Ligation PreMix is a lyophilized master mix containing T4 DNA Ligase, ATP, reaction buffer, and patented stabilizer. This DNA ligation premix is conveniently aliquoted in strip-tubes for reactions; you need only add DNAs to be ligated and water. The reaction will work for DNA ligation for all applications: blunt cloning, sticky end cloning and TA cloning. The premix is stable up to four months at room temper…
Bioneer is pleased to offer our Custom Gene synthesis service to help customers save time and money while improving research results. Gene synthesis is the most cost effective way to enhance your research. In as little as 2 weeks, you can have your Gene cloned in hand and 100% sequence guaranteed. And with Bioneer’s great pricing it can cost less to order a synthetic gene from us than to buy all the kits and reagents necessary…
Gene cloning and subcloning of genes into any cloning vector you want - 100% sequence guaranteed.
The fastest and most cost effective way to construct a mutant library for protein structure and function research or to increase enzyme function is to use our Point Mutation Service combined with our Gene Synthesis Service. Site-directed mutation such as Point Mutation as well as deletion mutation services are available. For unsurpassed quality and value pricing, partner with Bioneer today!
For programmable dispensing, microinjection, perfusion or spritzing of reproducible microliter volumes. Pulse a valve quickly for microliter delivery, or leave it on for constant perfusion. The short delivery path saves expensive reagents.• Deliver microliter volumes starting with as little as 100 microliters of precious drugsSwitch between eight solutions in milliseconds from a single tip• Minimize cross-contamination with Ba…
This motorized micromanipulator with a rack mount controller unit is affordable yet offers advanced features found in manipulators costing thousands more. Custom engineered stepping motors, precision cross-roller bearing slides and proprietary worm gear capstan drives form the basis of the watch-like mechanical system. The controller provides power to the stage motors with a quiet linear power supply to minimize electrical noi…
The flagship in Sutter's line of precision micromanipulators, the motorized MP-285 is affordable yet offers advanced features found in manipulators costing thousands more. Custom engineered stepping motors, precision cross-roller bearing slides and proprietary worm gear capstan drives form the basis of the watch-like mechanical system. The controller provides power to the stage motors with a quiet linear power supply to minimi…
Developed through years of experience with the Flaming/Brown style micropipette pullers and infused with leading edge technology, Sutter is proud to introduce the P-1000 micropipette puller. The most obvious new feature is the color touchscreen display that provides an intuitive and full-featured interface. F E A T U R E S > P - 1 0 0 0Color touch-screen interfaceSafe heat mode to protect and extend filament lifePipette Cookbo…
A significant advance in the technology of fabrication of micropipettes, optical fiber probes, and nanospray tips, is offered with the P-2000 micropipette puller. The P-2000 integrates a CO2 laser-based heat source with the technology derived from our extensive experience with conventional pullers. This system offers capabilities unmatched by other pullers. While the P-2000 is suitable for working with most conventional glasse…
