Genomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.
The TaqPlus Precision polymerase mixture is an optimized blend of Stratagene cloned Pfu DNA polymerase and Taq2000 DNA polymerase, a highly purified, recombinant version of Taq DNA polymerase. The TaqPlus Precision PCR system exhibits the highest replication accuracy achieved using DNA polymerase mixtures.
The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping. The pBluescript II phagemids have an extensive polylinker with 21 unique restriction enzyme recognition sites. New cDNA synt…
pCMV-Script PCR cloning system is a polymerase chain reaction (PCR) cloning method that can be performed in 1-hour without adding bases to the primers. The kit permits the efficient cloning of PCR fragments with a high yield and a low rate of false positives. CMV promoter for high-level protein expression in mammalian cells Large multiple cloning site contains 15 unique sites Clone PCR products generated with any PCR e…
Agilent mammalian transfection kits provide all the necessary reagents to efficiently and rapidly conduct DNA transfection in mammalian cells. It offers a simplified protocol that achieves high efficiency transfections in a variety of cell lines.
Agilent's GeneSpring provides powerful, accessible statistical tools for intuitive data analysis and visualization.
SureCall is a research desktop application combining both novel and widely accepted opensource algorithms for end-to-end NGS data analysis from alignment to categorization and annotation of mutations, supporting Agilent Target Enrichment applications.
The MBS Mammalian Transfection Kit increases transfection efficiencies by up to 100-fold. Cells are incubated with CaPO4/DNA coprecipitate for only 3 hours compared to 6 to 12 hours with traditional CaPO4 methods.
Easy-A Hi-Fi Cloning Enzyme & Master Mix combines the higher accuracy of a proofreading enzyme with the high cloning efficiency of Taq. PCR products amplified with the Easy-A can be cloned directly, without performing additional steps typically required when amplifying with proofreading polymerases.
The In Situ beta-Galactosidase Staining Kit provides an easy and rapid method to determine transfection efficiency by indicating beta-Galactosidase activity in individual intact cells. In situ staining of cells directly in dishes without removing or manipulating cells Rapid detection of Ÿ-gal activity in transfected cells Results in 24 hours Maximum signal with low background Deep blue staining reaction Kit in…
Discover AccuScript high-fidelity reverse transcriptase (RT). AccuScript delivers 3.7X more accuracy than other commercially available RTs and 6.6X more accuracy than AMV RT. The AccuScript PfuUltra II RT-PCR kit provides a complete system in applications that demand high fidelity, specificity, and throughput.
The AffinityScript qPCR cDNA Synthesis Kit offers efficient conversion of RNA to cDNA. Fully optimized for real-time quantitative PCR (qPCR) applications, the kit utilizes our qPCR-grade AffinityScript Multiple Temperature Reverse Transcriptase. It includes a buffer system optimized for real-time qPCR.
The AffinityScript qPCR cDNA Synthesis Kit offers efficient conversion of RNA to cDNA. Fully optimized for real-time quantitative PCR (qPCR) applications, the kit utilizes our qPCR-grade AffinityScript Multiple Temperature Reverse Transcriptase. It includes a buffer system optimized for real-time qPCR.
The Just cDNA Double-Stranded cDNA Synthesis Kit generates high-quality, blunt-ended cDNA, perfect for use in non-directional cDNA libraries. Makes blunt double-stranded cDNA Can be used for in vitro transcription Perfect for nondirectional cDNA libraries
QuikHyb hybridization solution was developed for use in Southern, Northern, slot-blot and plaque-lift formats using randomly labeled radioactive and nonradioactive nucleic acid probes. Reduces hybridization time from customary 12-24 hours to 1-2 hours Efficient and sensitive radioactive and nonradioactive detection of nucleic acids Doubles signal intensity
Agilent's GeneSpring Workgroup provides a robust data management and analysis environment for GeneSpring data and analysis results. It combines a highly scalable architecture, a rich set of analysis tools, and a series of programmatic interfaces that enable multi-campus enterprises to maximize the value of their research. Store and protect GeneSpring multi-omics data and analysis results Publish static images Secure ac…
Our single-stranded DNA binding standard is a helix-destabilizing E. coli protein. The 75.6-kD protein is essential for replication, recombination, and repair processes.
The HybriZAP-2.1 two-hybrid vector system, a eukaryotic system to detect proteinprotein interactions in vivo, provides a method for the rapid identification of genes encoding proteins that interact with a bait protein. The system is based on the ability to separate eukaryotic transcriptional activators into two separate domains, the DNA binding domain (BD) and the transcriptional activation domain (AD). Detects protein-prote…
Randomly pre-sheared DNA Molecular weight ranges from 300 bp to 2000 bp Guaranteed DNase-free
G418 blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells. G418 Sulfate is available as a crystalline solid or in aqueous sterile solution.
MVP RNA is application-ready RNA available in small economical pack sizes. Extensive and rigorous quality control including many application specific assays provides assurance that the RNA is intact, full-length and nearly DNA-free. Extensive quality control ensures high-quality RNA Eliminates tedious, time consuming RNA isolation procedures Application ready for real time RT-PCR, miRNA detection and Northern blot anal…
The Universal Human miRNA Reference RNA is an ideal reference control for miRNA microarray or miRNA-targeted QRTPCR experiments. The Agilent miRNA Kit may also be used as an optimization or standardization reagent for human miRNA analysis. Available in industrial lots for high throughout applications.
Identify fish species quickly, easily, and accurately. Whether your samples are fresh, frozen, dried, salted, or minced, Agilent’s DNA Fish Species Identification solution provides reliable, robust answers within hours. And, the method is far more accurate than looking at a fish and guessing.
The LightSwitch promoter reporter GoClone collection includes transfection-ready luciferase reporter constructs utilizing the novel RenSP luciferase technology for over 17,000 human promoters and highly optimized LightSwitch reagents. Measure the functional response of promoters to stimulus in nearly any cell-based system. Plus, advances in gene assay reagent and transfection technology eliminates the need for co-transfection…
An arrayed or non-arrayed BAC library is the preferred choice for downstream applications such as hybridization screening; full-genome sequencing; BAC end sequencing or physical mapping projects. Amount of clones received is based on a guaranteed coverage and 120Kb average insert size. HMW DNA isolation and QC by HMW DNA enzymatic digestion are included. Deliverables for ARRAYED BAC library: Clones will be arrayed and amplifie…
A pooled BAC library is an invaluable tool for PCR-screening. It allows for the easy retrieval of BAC clones of interest. This type of library is especially useful when genome size is large and creating an arrayed library is cost-prohibitive. We typically construct a minimum 5X BAC library and BAC clones are then pooled and distributed in 96-well plates. Each well will contain about 400-500 original BAC clones. HMW DNA isolati…
