PCR and Thermal Cycling Products & Reviews

Ensure your working area is organized and efficient with Agilent Tube Racks. Our PCR Tube Storage Racks provide high quality and robust tube storage for a range of working environments.

The passive reference dye can be added to fluorescence-based quantitative PCR (qPCR) assays to compensate for non-PCR related variations in fluorescence. Product comes in 10 vials of 100 µL. 1mM for controlling the final dye concentration.

Mx3000P & Mx3005P plates, tubes, filter sets, and replacement lamp assemblies.  96-well QPCR Plates, 8-Strip Tubes, Single Tubes, and Optically Clear Caps  Benchtop Racks for QPCR Tubes  Replacement Lamp Assembly

Ensure your working area is organized and efficient with Agilent Tube Racks. Our PCR Tube Storage Racks provide high quality and robust tube storage for a range of working environments. qPCR Tube Racks are available for 600 µL tubes (holding 40 x 600 µL tubes) and for 200 µL tubes or V-bottom plates.

Mx4000 plates, tubes, filter sets, and replacement lamp assemblies.  96-well QPCR Plates, 8-Strip Tubes, Single Tubes, and Optically Clear Caps  Benchtop Racks for QPCR Tubes  Additional Filter Sets  Replacement Lamp Assembly

Mx4000 plates, tubes, filter sets, and replacement lamp assemblies.  96-well QPCR Plates, 8-Strip Tubes, Single Tubes, and Optically Clear Caps  Benchtop Racks for QPCR Tubes  Additional Filter Sets  Replacement Lamp Assembly

Mx4000 plates, tubes, filter sets, and replacement lamp assemblies.  96-well QPCR Plates, 8-Strip Tubes, Single Tubes, and Optically Clear Caps  Benchtop Racks for QPCR Tubes  Additional Filter Sets  Replacement Lamp Assembly

Mx4000 plates, tubes, filter sets, and replacement lamp assemblies.  96-well QPCR Plates, 8-Strip Tubes, Single Tubes, and Optically Clear Caps  Benchtop Racks for QPCR Tubes  Additional Filter Sets  Replacement Lamp Assembly

Our new Service Program ensures you have a fully functional Mx3000P or Mx3005P QPCR System within 2 business days of reporting a problem. This maximizes the amount of time your QPCR system is up and running, ensuring an uninterrupted workflow and quicker response time than any other service program currently available in the industry.  Factory Service Center repair with loaner instrument for duration of repair  Guaranteed l…

The Agilent High-Specificity miRNA QRT-PCR Detection Kit and miRNA QRT-PCR Master Mix Detection Kit provide qualified reagents to polyadenylation of microRNAs (miRNAs) followed by synthesis of first-strand cDNA from these tailed miRNAs. A novel PCR enzyme formulation and qPCR detection reagents give utmost specificity.

The universal reverse primer is designed for use with the Human U6 forward primer provided in our High-Specificity miRNA QRT-PCR Detection Kit for a complete, validated assay requiring no upfront design work. The Kit delivers sensitive and reproducible detection of miRNA with high specificity.

Our miRNA qRT-PCR detection system enables accurate and real-time PCR quantification for miRNA. The kit is a high-specificity reagent system that delivers sensitive and reproducible results. The kit elongates miRNAs in a polyadenylation reaction and then reverse transcribes the polyadenylated RNA into QPCR-ready cDNA.

Agilent miRNA qPCR Detection Reagents are high-specificity reagents for the polyadenylation of microRNAs (miRNAs) from total RNA. The miRNA qPCR Detection Reagents also enable the synthesis of first-strand cDNA from the tailed miRNAs.

Our SideStep II QRT-PCR products deliver efficient cell lysis and sensitive quantitative gene expression analysis without RNA purification. Utilizing Agilent’s Brilliant QRT-PCR technology, multiple samples can be screened quickly and easily. The one-step process results in RNA stabilized for up to 6 months.

Our SideStep Lysis and Stabilization Buffer ensures accurate gene expression analysis by eliminating sample loss and degradation. The proprietary buffer lyses cells and immediately stabilizes the released nucleic acid for months. Several formats are available to enhance research versatility.

The Paq5000 DNA polymerase is a robust Taq substitute that is derived from a Pyrococcus species. This enzyme provides equivalent and often better performance to endpoint Taq DNA polymerase amplification and can be substituted easily into many Taq-based procedures.  Robust, cost-effective alternative to Taq  Equal to better yield with faster cycling conditions  Amplify up to 6 kb genomic DNA targets  Easily substituted…

The TaqPlus Precision polymerase mixture is an optimized blend of Stratagene cloned Pfu DNA polymerase and Taq2000 DNA polymerase, a highly purified, recombinant version of Taq DNA polymerase. The TaqPlus Precision PCR system exhibits the highest replication accuracy achieved using DNA polymerase mixtures.

Easy-A Hi-Fi Cloning Enzyme & Master Mix combines the higher accuracy of a proofreading enzyme with the high cloning efficiency of Taq. PCR products amplified with the Easy-A can be cloned directly, without performing additional steps typically required when amplifying with proofreading polymerases.

Discover AccuScript high-fidelity reverse transcriptase (RT). AccuScript delivers 3.7X more accuracy than other commercially available RTs and 6.6X more accuracy than AMV RT. The AccuScript PfuUltra II RT-PCR kit provides a complete system in applications that demand high fidelity, specificity, and throughput.

The AffinityScript qPCR cDNA Synthesis Kit offers efficient conversion of RNA to cDNA. Fully optimized for real-time quantitative PCR (qPCR) applications, the kit utilizes our qPCR-grade AffinityScript Multiple Temperature Reverse Transcriptase. It includes a buffer system optimized for real-time qPCR.

The AffinityScript qPCR cDNA Synthesis Kit offers efficient conversion of RNA to cDNA. Fully optimized for real-time quantitative PCR (qPCR) applications, the kit utilizes our qPCR-grade AffinityScript Multiple Temperature Reverse Transcriptase. It includes a buffer system optimized for real-time qPCR.

The Just cDNA Double-Stranded cDNA Synthesis Kit generates high-quality, blunt-ended cDNA, perfect for use in non-directional cDNA libraries.  Makes blunt double-stranded cDNA  Can be used for in vitro transcription  Perfect for nondirectional cDNA libraries

QuikHyb hybridization solution was developed for use in Southern, Northern, slot-blot and plaque-lift formats using randomly labeled radioactive and nonradioactive nucleic acid probes.  Reduces hybridization time from customary 12-24 hours to 1-2 hours  Efficient and sensitive radioactive and nonradioactive detection of nucleic acids  Doubles signal intensity

Taq Extender PCR additive improves the reliability and yield of conventional Taq-based PCR amplifications. Taq Extender additive increases the efficiency of Taq DNA polymerase extension reactions during each cycle of PCR by reducing mismatch pausing, resulting in a greater percentage of completed extension reactions.

The PCR grade deoxynucleotide mix includes 25 mM of each standard dNTP (GATC) for use with any PCR application, including real-time quantitative PCR. These reactions buffers contain the optimal magnesium concentration for efficient PCR.