Enzymes Products & Reviews

The engineered PfuUltra II Fusion HS DNA Polymerase provides the worlds most accurate copies and the fastest extension times possible. PfuUltra II Fusion HS DNA Polymerase is PfuUltra hotstart DNA polymerase coupled with a fusion polymerase technology and ArchaeMaxx PCR enhancing factor.  The industry standard for ultra high fidelity PCR providing the most accurate copies of DNA  Dramatically reduced extension times allow f…

These reactions buffers contain the optimal magnesium concentration for efficient PCR.  Ideal for optimal PCR performance  Standard 10x formulation  Optimized for use with specific PCR enzymes

Polyphosphates for a variety of applications.

The FabRICATOR Enzyme is supplied as a lyophilized powder with no preservatives added, and is available in 2000 and 5000 unit vials for digestion of up to 2 - 5 mg IgG.

β-D-Glucose:oxygen 1-oxidoreductase, G.Od., GOx

Having supplied restriction enzymes to the research community for over 45 years, NEB has earned the reputation of being the leader in enzyme technologies.  Working continuously to be worthy of that distinction, NEB strives to develop enzyme of the highest purity and unparalleled quality.  Time-Saver™ qualified for digestion in 5-15 minutes 100% activity in rCutSmart™ Buffer (over 215 enzymes are available in the same b…

HighTherm™ Reverse Transcriptase

A novel inhibitor of kinesin-like protein KIFC1

Thermo Scientific MMuLV (Moloney Murine Leukemia Virus) Reverse Transcriptase synthesizes a complementary DNA strand from single-stranded RNA. It is isolated from a clone over-expressing the gene from Moloney Murine Leukemia Virus. Features Good dynamic range Low RNase H activity ApplicationscDNA SynthesisRT-PCR

Thermo Scientific Proteinase K is a versatile and non-specific endolytic protease and is an essential tool for a wide array of proteolytic applications. Applications • General digestion of proteins • DNase and RNase inactivation • Cell digestion • Cell surface protein and glycoprotein modifications • Protein structure studies

AMV Reverse Transcriptase is qualified for cDNA synthesis and also for dideoxy sequencing of DNA and RNA. Using AMV Reverse Transcriptase, the reverse transcription step can be carried out at elevated temperatures up to 70°C. High temperature increases the specificity of priming and helps to increase the efficiency by reducing RNA secondary structures. ■ Compatible with 4mM sodium pyrophosphate and placental RNase inhibitor in…

T4 DNA Polymerase catalyzes the synthesis of DNA in the 5´→ 3´ direction and requires the presence of template and primer. This enzyme has a 3´→ 5´ exonuclease activity which is much more active than that found in DNA Polymerase I. Unlike E. coli DNA Polymerase I, T4 DNA Polymerase does not have a 5´→ 3´ exonuclease function. Source: Purified from a strain of E. coli that carries a T4 DNA Polymerase overproducing plasmid. Appl…

NovaTaq™ Hot Start DNA Polymerase is a chemically modified form of Taq DNA polymerase that is inactive at ambient temperature.

lyophilized powder, Protein ≥85 %, 

A potent, cell permeable, non-tubulin-interacting mitosis inhibitor. Arrests mammalian cells in mitosis with monopolar spindles. In vitro it specifically inhibits the motility of the mitotic kinesin Eg5, a motor protein involved in the assemble and maintainance of the mitotic spindle.

EpiScript™ Rnase H- Reverse Transcriptase (EpiScript RT) is a recombinant MMLV reverse transcriptase with greatly reduced RNase H activity. It is highly efficient at producing full-length cDNA from long RNA templates. EpiScript RT is capable of producing cDNA from as little as 50 pg of total RNA for real-time RT-PCR (qRT-PCR) analysis and other applications.

A workhorse, high quality DNA ligase for joining blunt or cohesive ends of dsDNA or dsRNA.

Reliable hot start Taq DNA Polymerase for sensitive detection of low abundance target DNA. Ideal for standard end point PCR and qPCR applications. Available in Glycerol-free, Triton-free, and high concentration formulations.

T7 RNA Polymerase catalyzes the 5′→3′ RNA synthesis from the T7 promoter. It is a DNA-dependent RNA polymerase cloned from the T7 bacteriophage, which recognizes the T7 promoter and terminator sequences with high specificity. Key features:  Reliable transcription 

Protect RNA with this high quality, effective RNase inhibitor.

25 x 20 µl reactions, includes 100 µl iScript Advanced 5x Reaction Mix, 25 µl iScript Advanced Reverse Transcriptase

25 x 20 µl reactions, 100 µl 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 200 µl No-RT control supermix; 12.5 µl DNase; 150 µl DNase buffer

100 x 20 µl reactions, 400 µl 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 400 µl No-RT control supermix; 50 µl DNase; 150 µl DNase buffer

50 x 50 µl reactions, contains iScript Advanced Reverse Transcriptase, 2x PreAmp Mix (dNTPs, Sso7d fusion polymerase, salts, enhancers, stabilizers, and other proprietary components), reaction booster, DNase, DNase buffer, nuclease-free water

250 x 50 µl reactions, contains iScript Advanced Reverse Transcriptase, 2x PreAmp Mix (dNTPs, Sso7d fusion polymerase, salts, enhancers, stabilizers, and other proprietary components), reaction booster, DNase, DNase buffer, nuclease-free water